Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,924,004	T→C	I544V (ATT→GTT)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,924,004	0	T	C	100.0%	178.4 / NA	50	I544V (ATT→GTT)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/22);  total (28/22)

GCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGG  >  NC_000913/3923871‑3924140                                                                                                                                      |                                                                                                                                         gCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGTGCGCGATCTAAGCTGCGTATTTAACCTGAACCTCAAc                                                                                                                                    >  1:410656/1‑140 (MQ=255) gCGATCACTTCATTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAAc                                                                                                                                    >  2:579596/1‑140 (MQ=255)  cGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGTCGCGCGACATAACCTTAGTATTTAACCTGAACCTCAAcc                                                                                                                                   >  2:350354/1‑140 (MQ=255)     gagacaggTTAGAAAGACCGGACACCTGGCGGTAATCCACTGTCGCGGCTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGt                                                                                                                                <  2:396787/133‑1 (MQ=255)         ttCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTccg                                                                                                                            <  1:822838/140‑1 (MQ=255)          tCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTccgc                                                                                                                           <  1:817426/140‑1 (MQ=255)           cGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTGAACCTGAACGTCAACCTGTTccgcc                                                                                                                          >  1:433441/1‑140 (MQ=255)            gTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTccgccg                                                                                                                         >  1:642141/1‑140 (MQ=255)             ttAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCcgccgc                                                                                                                        <  1:220309/140‑1 (MQ=255)                   aGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTc                                                                                                                  >  2:74032/1‑140 (MQ=255)                     aCCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGt                                                                                                                <  1:759847/140‑1 (MQ=255)                      ccGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATTACCTTCGTGTTTTACCGGAACCTCAGCCTGATCCGCCGCCTGTTCGTc                                                                                                               >  2:265135/1‑140 (MQ=255)                      ccGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTc                                                                                                               >  2:979077/1‑140 (MQ=255)                      ccGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTc                                                                                                               <  1:74032/140‑1 (MQ=255)                       cGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGACCCTTAAGCTGTTCCGCCGCCTgctgtctc                                                                                                              >  1:396787/1‑133 (MQ=255)                          aTACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTc                                                                                                           >  1:890148/1‑140 (MQ=255)                               tGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTCTTCGTCTGTCAACGc                                                                                                      >  2:807510/1‑140 (MQ=255)                                    gTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAgggg                                                                                                 >  1:235865/1‑140 (MQ=255)                                    gTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAgggg                                                                                                 <  1:904468/140‑1 (MQ=255)                                            gtgtCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGGTCCACCGCCTGTTCGTGTGTCTACGCAGGGGCAAACGGc                                                                                         >  2:378879/1‑140 (MQ=255)                                                 gcgGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAg                                                                                    <  2:973998/140‑1 (MQ=255)                                                  cgGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCaaa                                                                                             <  2:758474/130‑1 (MQ=255)                                                  cgGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCaaa                                                                                             >  1:758474/1‑130 (MQ=255)                                                       aGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGt                                                                              <  2:881008/140‑1 (MQ=255)                                                              tgtTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAAtttt                                                                       >  1:955348/1‑140 (MQ=255)                                                                ttCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGt                                                                                       >  2:901207/1‑122 (MQ=255)                                                                ttCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGt                                                                                       <  1:901207/122‑1 (MQ=255)                                                                      ttACGCAGCTGCTTTCCGATCTCATCTTGATGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGt                                                               <  1:350354/140‑1 (MQ=255)                                                                        aCGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCa                                                             >  1:238129/1‑140 (MQ=255)                                                                                gctTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTCTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGga                                                     <  1:807510/140‑1 (MQ=255)                                                                                gctTTTCGATCTCATCTTGCTGGCGCGCGATATAAACTTTGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGga                                                     <  2:433441/140‑1 (MQ=255)                                                                                         tctcATCTTGCTGGCGCCCGATATAACCTTCTTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACgcagc                                            <  2:434448/140‑1 (MQ=255)                                                                                             aTCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCATCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGAt                                        >  2:239973/1‑140 (MQ=255)                                                                                                   cTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTcac                                  >  2:634555/1‑140 (MQ=255)                                                                                                   cTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTcac                                  >  1:720529/1‑140 (MQ=255)                                                                                                     ggCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTcacca                                <  1:634555/140‑1 (MQ=255)                                                                                                       cgcgcgATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACt                              >  2:1001924/1‑140 (MQ=255)                                                                                                       cgcgcgATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACt                              >  1:140691/1‑140 (MQ=255)                                                                                                                       cGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAgc              >  1:27932/1‑140 (MQ=255)                                                                                                                        gTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCa                       >  2:184030/1‑130 (MQ=255)                                                                                                                        gTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCa                       <  1:184030/130‑1 (MQ=255)                                                                                                                        gTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAgcg             >  2:877729/1‑140 (MQ=255)                                                                                                                        gTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAgcg             >  2:998189/1‑140 (MQ=255)                                                                                                                        gTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAgcg             <  2:642141/140‑1 (MQ=255)                                                                                                                        gTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAgcg             <  2:218024/140‑1 (MQ=255)                                                                                                                        gTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAgcg             >  1:218024/1‑140 (MQ=255)                                                                                                                          aTTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAgcggc           <  2:754174/140‑1 (MQ=255)                                                                                                                            ttAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGACAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGgcgc         <  1:979077/140‑1 (MQ=255)                                                                                                                              aaCCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCACAg       >  1:121240/1‑140 (MQ=255)                                                                                                                              aaCCTGAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAAATCATCACCACTCGCTTCACGGGAAAGCGGCGCAg       >  2:434349/1‑140 (MQ=255)                                                                                                                                   gAACCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAgg  <  1:998189/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGTTAATTTTTCATAAGTCATTTCCGGACGACGCAGCAGATCTTCACCACTGGCTTCACGGGAAAGCGGCGCAGTCAGG  >  NC_000913/3923871‑3924140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑