Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	406,193	T→C	W144R (TGG→CGG)	yaiI →	DUF188 domain‑containing protein YaiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	406,193	0	T	C	100.0%	282.4 / NA	79	W144R (TGG→CGG)	yaiI	DUF188 domain‑containing protein YaiI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/48);  total (31/48)

GCCAGCGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGTGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTA  >  NC_000913/406057‑406329                                                                                                                                         |                                                                                                                                         gCCAGCGACCATTCGTGAGCGCTTGACGATGTGCGTTTTTAAGGATACATTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTggcggc                                                                                                                                       <  2:424976/140‑1 (MQ=255)    aGCGACCATTCGTGAGCTCCTGCCGATGCGCCATTTTATGGATACCTTGCGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTgg                                                                                                                                    <  2:373459/140‑1 (MQ=255)    aGCGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTgg                                                                                                                                    <  1:874313/140‑1 (MQ=255)    aGCGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTgg                                                                                                                                    <  1:506443/140‑1 (MQ=255)      cGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGaa                                                                                                                                  >  1:944362/1‑140 (MQ=255)      cGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGaa                                                                                                                                  >  1:676849/1‑140 (MQ=255)      cGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGaa                                                                                                                                  >  1:645124/1‑140 (MQ=255)      cGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGaa                                                                                                                                  >  2:604605/1‑140 (MQ=255)      cGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGaa                                                                                                                                  >  1:571289/1‑140 (MQ=255)         ccATTCGTGTGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTg                                                                                                                               <  2:593247/140‑1 (MQ=255)         ccATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTGCGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTg                                                                                                                               <  2:112971/140‑1 (MQ=255)         ccATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGTCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGCAAGTg                                                                                                                               <  1:858953/140‑1 (MQ=255)         ccATTCGTGAGCGCCCGACGATACGTGATCTTATGGACACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTg                                                                                                                               <  1:262442/140‑1 (MQ=255)             tCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAAc                                                                                                                           >  2:696176/1‑140 (MQ=255)                  aGCGCCTGACGATGCGCAATTTAATGGATACCTTACGTGGCAGTGGGACCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGt                                                                                                                      <  2:435123/140‑1 (MQ=255)                    cgcCTGACGATGCGCGATTTTATGGATTCCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGGGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCg                                                                                                                    <  2:944362/140‑1 (MQ=255)                    cgcCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCg                                                                                                                    <  2:704609/140‑1 (MQ=255)                    cgcCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCg                                                                                                                    <  1:696176/140‑1 (MQ=255)                    cgcCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGGGGCGGCTGGAAGTGCAACGTAGTCg                                                                                                                    >  1:404542/1‑140 (MQ=255)                      ccTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTg                                                                                                                               <  1:521836/127‑1 (MQ=255)                      ccTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTg                                                                                                                               >  2:521836/1‑127 (MQ=255)                       cTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTgg                                                                                                                 <  2:636238/140‑1 (MQ=255)                         gACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCt                                                                                                               <  1:667010/140‑1 (MQ=255)                                    aTTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAAttt                                                                                                    <  1:615427/140‑1 (MQ=255)                                    aTTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTAGAAGTGCAACGTAGTCGGGGCTCAATGTAAttt                                                                                                    >  2:927711/1‑140 (MQ=255)                                       ttATGGATACCTTACGGGCCAGGGGGATCCACAGCAGCGGACCAGATACCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATttatt                                                                                                 <  2:447232/140‑1 (MQ=255)                                          tGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTattatt                                                                                              <  1:951363/140‑1 (MQ=255)                                            gATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTa                                                                                            <  2:192049/140‑1 (MQ=255)                                               aCCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTacac                                                                                         >  1:23339/1‑140 (MQ=255)                                                ccTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACt                                                                                        <  2:972969/140‑1 (MQ=255)                                                 cTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACtt                                                                                       >  2:539165/1‑140 (MQ=255)                                                   tACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCa                                                                                     <  1:815129/140‑1 (MQ=255)                                                    aCGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCAt                                                                                    >  2:911650/1‑140 (MQ=255)                                                      gTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAGGTGCAACGCAGTAGTGTCTAAATGTGATTTATTATTTACACTTCATTc                                                                                  >  2:349514/1‑140 (MQ=255)                                                       tGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGGGCAACGTGGTCATGGGTAAATGTAATTTAGTATTTACACTTCATTCt                                                                                 >  2:275488/1‑140 (MQ=255)                                                         ccAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTg                                                                               >  1:212244/1‑140 (MQ=255)                                                          cAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGa                                                                              <  2:23339/140‑1 (MQ=255)                                                          cAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGa                                                                              <  2:676849/140‑1 (MQ=255)                                                           aGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGaa                                                                             >  2:967056/1‑140 (MQ=255)                                                           aGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCCGGAAGTGCAACGTAGTCCTGGATAAATGTAATTTTTTATTTACACTTCATTCTTGaa                                                                             >  2:449135/1‑140 (MQ=255)                                                             tGGGATCCAGACCGGCGGCCCGGATAGCCTTTCACAACGTGACAGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAata                                                                           <  2:690533/140‑1 (MQ=255)                                                             tGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAata                                                                           <  2:212244/140‑1 (MQ=255)                                                                 aTCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtattta                                                                       <  1:911650/140‑1 (MQ=255)                                                                 aTCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtattta                                                                       >  1:72089/1‑140 (MQ=255)                                                                  tCCAGACCGGCGGACCAGATAGTCTGTCATAACGTGATCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtatttat                                                                      <  1:349514/140‑1 (MQ=255)                                                                  tCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGCAGTGCAACGTAGCCGTGGCTAGATGAAATATTTTATTTGAACTACTTTCTTGTAtattttt                                                                      >  2:497480/1‑138 (MQ=255)                                                                  tCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtatttat                                                                      >  2:554595/1‑140 (MQ=255)                                                                  tCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtatttat                                                                      <  1:132017/140‑1 (MQ=255)                                                                  tCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtatttat                                                                      >  2:900538/1‑140 (MQ=255)                                                                  tCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtatttat                                                                      <  2:803000/140‑1 (MQ=255)                                                                  tCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtatttat                                                                      <  2:692825/140‑1 (MQ=255)                                                                   ccAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAAtatttatt                                                                     <  1:967056/140‑1 (MQ=255)                                                                    cAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTg                                                                    <  1:604605/140‑1 (MQ=255)                                                                    cAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTg                                                                    >  2:509923/1‑140 (MQ=255)                                                                    cAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGGGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTg                                                                    >  1:690533/1‑140 (MQ=255)                                                                      gACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGt                                                                  <  2:779537/140‑1 (MQ=255)                                                                      gACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGt                                                                  <  2:79752/140‑1 (MQ=255)                                                                      gACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGt                                                                  <  2:571289/140‑1 (MQ=255)                                                                       aCCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGta                                                                 >  2:865606/1‑140 (MQ=255)                                                                       aCCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGGGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGta                                                                 >  2:173479/1‑140 (MQ=255)                                                                           gcggACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGt                                                             <  1:509923/140‑1 (MQ=255)                                                                            cggACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTTTTATTTACACTTCATTCTTGAATATTTATTGGTATAGTa                                                            >  2:419869/1‑140 (MQ=255)                                                                             ggACCAGATAGCCTTTCACAACTTGCCCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTaa                                                           <  1:275488/140‑1 (MQ=255)                                                                             ggACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTaa                                                           <  1:554595/140‑1 (MQ=255)                                                                                ccAGATAGCCTTTCACACCGTGCCCGCCAGGCCTTTGCCCCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAggg                                                        <  2:451817/140‑1 (MQ=255)                                                                                ccAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAggg                                                        <  2:585250/140‑1 (MQ=255)                                                                                ccAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAggg                                                        <  2:506496/140‑1 (MQ=255)                                                                                   gATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGtat                                                                >  1:74449/1‑129 (MQ=255)                                                                                   gATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGtat                                                                <  2:74449/129‑1 (MQ=255)                                                                                        ccTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTg                                                <  2:72089/140‑1 (MQ=255)                                                                                                  cGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTAtt                                                 <  1:788754/129‑1 (MQ=255)                                                                                                  cGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTAtt                                                 >  2:788754/1‑129 (MQ=255)                                                                                                  cGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTTAGATTTTCAc                                      >  1:936817/1‑140 (MQ=255)                                                                                                  cGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCAc                                      >  1:517511/1‑140 (MQ=255)                                                                                                                   tGCCGCGGAGCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAG‑TTTTCACTTTAAGTGGAATTTTTTc                    >  1:229278/1‑140 (MQ=255)                                                                                                                            gCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAAt            <  1:927711/140‑1 (MQ=255)                                                                                                                            gCTGGAGAAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAAt            <  1:539165/140‑1 (MQ=255)                                                                                                                            gCTGGAGAAGTGGCGGCCGGAAATGCAACGTTGTCCTGGCTAAATGTACTTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAAt            <  2:293576/140‑1 (MQ=255)                                                                                                                                gagaAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCCaa        <  2:645124/140‑1 (MQ=255)                                                                                                                                 agaAGTGGCGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGaaa       <  1:419869/140‑1 (MQ=255)                                                                                                                                     gtggcgGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGaaa       >  2:245732/1‑136 (MQ=255)                                                                                                                                     gtggcgGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGaaa       <  1:245732/136‑1 (MQ=255)                                                                                                                                     gtggcgGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGGGTATTGAGATTTTCACTTTAAGTGGAATATTTTTCTTACAATCGAAATTGt   >  2:464812/1‑140 (MQ=255)                                                                                                                                      tggcggCTGGAAGTGTAACGTACTCCTGGCTAAATGTGATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTa  <  1:324664/140‑1 (MQ=255)                                                                                                                                      tggcggCTGGAAGTGCAACGTATTCCTGGATAAATGTGATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTa  <  1:464812/140‑1 (MQ=255)                                                                                                                                      tggcggCTGGAAGTGCAACGTAGTCGTGGCTAAATGTCATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTa  <  1:497480/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCCAGCGACCATTCGTGAGCGCCTGACGATGCGCGATTTTATGGATACCTTACGTGCCAGTGGGATCCAGACCGGCGGACCAGATAGCCTTTCACAACGTGACCGCCAGGCCTTTGCCGCGGAGCTGGAGAAGTGGTGGCTGGAAGTGCAACGTAGTCGTGGCTAAATGTAATTTATTATTTACACTTCATTCTTGAATATTTATTGGTATAGTAAGGGGTGTATTGAGATTTTCACTTTAAGTGGAATTTTTTCTTTACAATCGAAATTGTA  >  NC_000913/406057‑406329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑