| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,997,767 | T→C | G195G (GGT→GGC) | yigB → | 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,997,767 | 0 | T | C | 96.1% | 165.1 / ‑2.8 | 51 | G195G (GGT→GGC) | yigB | 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (30/19); total (31/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGATATAATCAG > NC_000913/3997636‑3997903 | tttGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAAt > 2:562886/1‑140 (MQ=255) gtgCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCg > 1:136730/1‑140 (MQ=255) gtgCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCg > 1:626952/1‑140 (MQ=255) gtgCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCg > 1:41870/1‑140 (MQ=255) tGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGc > 2:159985/1‑140 (MQ=255) gCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACATGGGCGGGGCAATTCGCAGCGGAATGCAg > 1:364709/1‑140 (MQ=255) cACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCttg > 2:601983/1‑140 (MQ=255) cgcTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGCGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGAt < 2:592637/140‑1 (MQ=255) aaaaCCGTTCAGCGAAATGTACTTTTTGGTTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGAc < 1:405727/140‑1 (MQ=255) cAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATg < 2:41870/140‑1 (MQ=255) tttGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCaaa > 1:163316/1‑140 (MQ=255) tGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAAcc > 2:23124/1‑140 (MQ=255) ggCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCt > 2:662999/1‑140 (MQ=255) cTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTgg > 2:810846/1‑140 (MQ=255) gCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGa < 2:656928/140‑1 (MQ=255) gCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCAGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGa < 1:885645/140‑1 (MQ=255) gCGGAAAAACTCAACATGCCGAGCGCCGATATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGa < 1:413538/140‑1 (MQ=255) gAAAAACTCAACGTGCCGATCGTCGGGATATTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAg < 1:406515/140‑1 (MQ=255) aCGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTg > 2:26609/1‑140 (MQ=255) cGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCGGACCGGAACATGGCGATCTGATGCATACCTTGGACAGCCGTTTACTGc > 2:406515/1‑140 (MQ=255) gCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGc > 1:697530/1‑140 (MQ=255) gCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGc > 2:507650/1‑140 (MQ=255) gCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGc > 2:66998/1‑140 (MQ=255) gATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATc < 1:159985/140‑1 (MQ=255) cGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGCTGCAAACCTGGGACAGGCGTTCACTGCCGCATCTGGaaa > 2:432075/1‑140 (MQ=255) tGTTGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTgg > 1:518512/1‑140 (MQ=255) ttGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTcc > 2:49775/1‑131 (MQ=255) ttGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTcc < 1:49775/131‑1 (MQ=255) ttGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTgg < 2:619332/138‑1 (MQ=255) ttGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTgg > 1:619332/1‑138 (MQ=255) tGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCAt > 2:600934/1‑140 (MQ=255) tGGGGACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCCTTTGCTGCCGCAGCTGGGAATTTCCCGGTTGGCAt > 2:265967/1‑140 (MQ=255) ggggACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCCGGGACAGTCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCAtc > 2:576858/1‑140 (MQ=255) gggACGATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCAtct > 2:669710/1‑140 (MQ=255) gATCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGAc < 2:697530/140‑1 (MQ=255) aTCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGAcc > 1:946849/1‑140 (MQ=255) aTCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGAcc > 1:68809/1‑140 (MQ=255) aTCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGAc < 2:538524/139‑1 (MQ=255) aTCTCACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGAc > 1:538524/1‑139 (MQ=255) tctcACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGa > 1:696476/1‑137 (MQ=255) tctcACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGa < 2:696476/137‑1 (MQ=255) ctcACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTc < 1:600934/140‑1 (MQ=255) tcACCACTGACGTGGGCTGGGCCATTCGCAGAGGATTGCGGGCTTGTTCGATCAGACCGGAAAATCGCGATCTGATGCAAACCTGGAACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGATTGGCATCTCTGACCTCg < 2:497261/140‑1 (MQ=255) tcACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCg < 1:810846/140‑1 (MQ=255) tcACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCg < 2:626952/140‑1 (MQ=255) tcACCACTGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCg < 2:136730/140‑1 (MQ=255) ccacTGACGTGGGCGTGGCAATTCGTAGCGGAATGCAAGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTg < 1:432075/140‑1 (MQ=255) cTGACGTGGGCGGGGCATTTCGCAGCGGAATGCAGACTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGata < 2:408190/140‑1 (MQ=255) tGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGat > 2:930259/1‑138 (MQ=255) tGACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGat < 1:930259/138‑1 (MQ=255) gACGTGGGCGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGAtata > 1:789829/1‑140 (MQ=255) gACGTGGGCGGGGCAATTCGCAGCGGAAAGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGAtata > 2:611133/1‑140 (MQ=255) aCGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGATATaa > 2:142806/1‑140 (MQ=255) tgggcgggGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAAATTCCCGGTTGGTATCTCTGACCTCGCTGATATAATCa > 1:319727/1‑140 (MQ=255) gggcgggGCAATTCGCAGCGGAAAGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGATATAATCAg < 1:611133/140‑1 (MQ=255) | TTTGTGCTGCGCGCTGGCCCGCACGGGCGCTCAAAACCGTTCAGCGATATGTACTTTTTGGCTGCGGAAAAACTCAACGTGCCGATCGGCGAGATCTTACATGTTGGGGACGATCTCACCACTGACGTGGGTGGGGCAATTCGCAGCGGAATGCAGGCTTGTTGGATCAGACCGGAAAATGGCGATCTGATGCAAACCTGGGACAGCCGTTTACTGCCGCATCTGGAAATTTCCCGGTTGGCATCTCTGACCTCGCTGATATAATCAG > NC_000913/3997636‑3997903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |