Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,043,008	A→G	L198L (CTA→CTG)	srkA →	stress response kinase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,008	0	A	G	100.0%	112.0 / NA	32	L198L (CTA→CTG)	srkA	stress response kinase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/31);  total (1/31)

TTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCA  >  NC_000913/4042875‑4043117                                                                                                                                      |                                                                                                              ttGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGga                                                                                                         >  2:797972/1‑140 (MQ=255)   gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCAGCGTTCTGTGGCTGCGTGgagg                                                                                                       >  1:613156/1‑139 (MQ=255)   gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATg                                                                                                           >  1:32594/1‑136 (MQ=255)   gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATg                                                                                                           <  2:32594/136‑1 (MQ=255)   gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGgaga                                                                                                       >  1:790259/1‑140 (MQ=255)    aGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGAc                                                                                                      >  2:817240/1‑140 (MQ=255)      ccACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGCGGCCGCCTGGAGACTg                                                                                                    >  2:933246/1‑140 (MQ=255)       cACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGc                                                                                                   <  2:940558/140‑1 (MQ=255)       cACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGAGGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGc                                                                                                   <  1:212278/140‑1 (MQ=255)          gCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCAc                                                                                                >  1:648270/1‑140 (MQ=255)            aaGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGc                                                                                              <  1:614150/140‑1 (MQ=255)                      aGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGATCTGCGGATGCATGGGGACTGGCACGCCGGGAATAtt                                                                                    >  1:288078/1‑140 (MQ=255)                        gACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCTGCATGGGGACTGCCCCCCCGGGGATATtct                                                                                  >  1:235386/1‑140 (MQ=255)                            cTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTgg                                                                              <  1:117464/140‑1 (MQ=255)                            cTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTCACCGTTTTGCGGCTGCATGGAGAGTGCCACGCCGGGAAAATTttcttg                                                                              >  2:230715/1‑138 (MQ=255)                                acTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCCGCATGGAGACTGCCACGCCGGGAATATTCTCTGGcgcg                                                                          <  1:933246/140‑1 (MQ=255)                                 cTGATCCCTCCCGGGTTTAAAGCGGCATTCCAGAACGCGACAGATGAGATGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGa                                                                         <  2:288078/140‑1 (MQ=255)                                    aTACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTAACGTTTTGCGGCTGCTAGGTGGCTGCCCCACCTGGAATATTTTCTGGcgcggcgg                                                                      >  2:405793/1‑136 (MQ=255)                                    aTACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATgg                                                                      <  2:163282/140‑1 (MQ=255)                                       ccTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTcc                                                                   <  2:790259/140‑1 (MQ=255)                                        cTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCa                                                                  <  2:648270/140‑1 (MQ=255)                                        cTTCCGGGTTGAAAGCGCCATTCCTGAAAGCGAAATATGCGCTGATTGACGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCa                                                                  <  2:440740/140‑1 (MQ=255)                                          tCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTTTGCGGCTGCATGGAGACTGCCCCCCCGGGGAAATTTTCTTGCGCGATGGTCCCAt                                                                >  2:525908/1‑140 (MQ=255)                                          tCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAAt                                                                >  1:525612/1‑140 (MQ=255)                                          tCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAAt                                                                <  1:83010/140‑1 (MQ=255)                                          tCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACCCCCGGAATATTTTCTGGCGCGGTGGTGCaaa                                                                >  2:637710/1‑139 (MQ=255)                                            cGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGCAGATTTTACCCTTTTTCGGCGGCAAGGCGACGTCCACCGCCGGAATAATTTCTGGCGCGATCGTCCCAtgt                                                              >  2:175061/1‑140 (MQ=255)                                              ggTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAAtgttt                                                            <  2:837457/140‑1 (MQ=255)                                               gTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAAtgtttg                                                           <  1:797972/140‑1 (MQ=255)                                                    aaGCGGCATTCCTGAAAACGACCGATGAGCTTATTCCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGAt                                                      <  2:423288/140‑1 (MQ=255)                                                       cGGCATTCCTGAAAGCTACAGATGAGCTGATTGCCGCCGGTACAGCACCCTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTg                                                   <  2:449858/140‑1 (MQ=255)                                                         gCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGga                                                 <  1:817240/140‑1 (MQ=255)                                                          cATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGGGACTGCCAAGCCGGGGACATTCTCTAGCGCGGTGGTCCAATGTTTGTTGATCTGgag                                                >  2:326922/1‑139 (MQ=255)                                                           aTTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGgatg                                               <  1:637710/140‑1 (MQ=255)                                                              ccTGAAAGCGACAGATGAGCTGGTTGCCGGCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGatgatg                                            <  1:455200/140‑1 (MQ=255)                                                              ccTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGatgatg                                            <  1:212172/140‑1 (MQ=255)                                                               cTGAAAGCGACAGATGATCTGTCTGCCGCCGTTACTGCATACTGGCGGTAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGc                                           <  2:279394/140‑1 (MQ=255)                                                               cTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGc                                           <  2:525612/140‑1 (MQ=255)                                                                tGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCa                                          <  2:939727/140‑1 (MQ=255)                                                                   aaGCGAAAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGt                                       <  1:175061/140‑1 (MQ=255)                                                                    ggagACAGATGAGCTGATGGCCGCCGTTACAGCGCACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTa                                      <  2:613156/137‑1 (MQ=255)                                                                        aCAGATGAGCTGATTTCCGCCGTTACAGCACACTGGCTGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATgg                                  <  1:405793/140‑1 (MQ=255)                                                                        aCAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTTGGGCCGCCTGGAGGCTGCCACGCCGGGAATATTTTCTGGCGCGGTGGTCCAATGTTTTTTTATCTGGGTGGTGCACGGAAAgg                                  >  2:48187/1‑140 (MQ=255)                                                                             tGAGCTGATTGCCGCTGTTACACCAGACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAg                             <  1:307970/140‑1 (MQ=255)                                                                             tGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAg                             <  1:865387/140‑1 (MQ=255)                                                                                gCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCGCCTGGGGACTGCCACCCCGGGGAAATTCTCTGGCGCGATGGTCCCATGTTTTTTTGTCTGGATGATGCACGTAATGGGCccgccg                          >  2:647896/1‑140 (MQ=255)                                                                                        ccgccgTTGCGGCACACGGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGGCTGCCTCGCCGGGAAGATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCGTTCAGGAt                  <  2:891583/140‑1 (MQ=255)                                                                                                      cacTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCt    <  2:235386/140‑1 (MQ=255)                                                                                                       acTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTc   <  1:728593/140‑1 (MQ=255)                                                                                                        cTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGTGGGCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGGTCAGGGTTTTTGGATGTTTCTCa  >  2:201758/1‑140 (MQ=255)                                                                                                        cTGGCGGGAAGATTTCACCGTTCTGCGGCTGCATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCa  <  1:943271/140‑1 (MQ=255)                                                                                                                                      |                                                                                                              TTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCA  >  NC_000913/4042875‑4043117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑