Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,163,215	A→G	V19A (GTG→GCG)	trmA ←	tRNA m(5)U54 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,163,215	0	A	G	100.0%	266.9 / NA	74	V19A (GTG→GCG)	trmA	tRNA m(5)U54 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/39);  total (35/39)

CGAAAATGATGTGATACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCACTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTG  >  NC_000913/4163078‑4163351                                                                                                                                          |                                                                                                                                         cGAAAATGATGTGATACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCt                                                                                                                                        >  2:682469/1‑140 (MQ=255) cGAAAATGATGTGATACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCt                                                                                                                                        >  2:596929/1‑140 (MQ=255)    aaaTGATGTGATACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCtttt                                                                                                                                     <  1:179688/140‑1 (MQ=255)            tgATACAGGTCATCGCCATCGTCCCAGATGCTGAAGTCCCCGCGCATCTGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTaa                                                                                                                             <  2:406954/140‑1 (MQ=255)              aTACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAATGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACt                                                                                                                           >  2:868304/1‑140 (MQ=255)              aTACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATCCTTTGCAATCGTAGCGCTTTTTCGGCTAACt                                                                                                                           >  2:285769/1‑140 (MQ=255)              aTACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACt                                                                                                                           <  1:1019942/140‑1 (MQ=255)              aTACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACt                                                                                                                           <  2:914231/140‑1 (MQ=255)                  aGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTgcgc                                                                                                                       >  1:975221/1‑140 (MQ=255)                    gTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCt                                                                                                                               <  1:802575/130‑1 (MQ=255)                    gTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCt                                                                                                                               >  2:802575/1‑130 (MQ=255)                    gTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCtt                                                                                                                     >  2:166840/1‑140 (MQ=255)                      cATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCg                                                                                                                                  >  1:245341/1‑125 (MQ=255)                      cATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCg                                                                                                                                  <  2:245341/125‑1 (MQ=255)                        tCGCCATCGTGCCAGATGCGGACCTCCGCGCCCATTCGGCAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATa                                                                                                                 <  2:463149/140‑1 (MQ=255)                        tCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATa                                                                                                                 >  2:969553/1‑140 (MQ=255)                        tCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTCTTTCGGATAACTGCGCTTCATa                                                                                                                 >  1:463149/1‑140 (MQ=255)                         cGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCGATCATACTGTGCGAACGTACCGCTTCTTCGGCTAACTGCGCTTCATAc                                                                                                                >  2:284113/1‑140 (MQ=255)                         cGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATAc                                                                                                                >  2:726006/1‑140 (MQ=255)                         cGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATAc                                                                                                                >  2:388280/1‑140 (MQ=255)                          gCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATAct                                                                                                               >  1:562471/1‑140 (MQ=255)                           ccATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATActg                                                                                                              <  2:224836/140‑1 (MQ=255)                             aTCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATActgtt                                                                                                            <  2:1000508/140‑1 (MQ=255)                             aTCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATActgtt                                                                                                            <  1:969553/140‑1 (MQ=255)                             aTCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATActgtt                                                                                                            <  2:975221/140‑1 (MQ=255)                               cGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATActgttct                                                                                                          >  2:1017618/1‑140 (MQ=255)                                   ccAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTg                                                                                                      >  1:894828/1‑140 (MQ=255)                                      gATGCGGAACTCCGCGCGCATCCGGGAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGaa                                                                                                   >  2:705494/1‑140 (MQ=255)                                       aTGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAg                                                                                                  <  1:103059/140‑1 (MQ=255)                                       aTGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAg                                                                                                  <  1:166840/140‑1 (MQ=255)                                       aTGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAg                                                                                                  <  1:504455/140‑1 (MQ=255)                                            gAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGtt                                                                                             <  1:1017618/140‑1 (MQ=255)                                              aCTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCg                                                                                           <  2:897846/140‑1 (MQ=255)                                                  cgcgcgCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCggggg                                                                                       >  2:64813/1‑140 (MQ=255)                                                  cgcgcgCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCggggg                                                                                       >  1:649012/1‑140 (MQ=255)                                                   gcgcgcATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGt                                                                                      <  2:881329/140‑1 (MQ=255)                                                    cgcgcATCTGGTAATGGTTGACCGGCGAGCGGACCACTTCCGGAACCACGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTc                                                                                     <  2:468691/140‑1 (MQ=255)                                                    cgcgcATCCGGTAATGATTGACTGGCGAGCGCAACTCTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTc                                                                                     <  2:412652/140‑1 (MQ=255)                                                    cgcgcATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTc                                                                                     <  2:562471/140‑1 (MQ=255)                                                         aTCCGGTAATGACTGACCGGCGAGCTAAACACTTCCGCAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCatgat                                                                                <  1:309658/140‑1 (MQ=255)                                                         aTCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCatgat                                                                                >  2:792033/1‑140 (MQ=255)                                                                 aTGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTg                                                                        <  2:801657/140‑1 (MQ=255)                                                                 aTGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTg                                                                        <  2:649012/140‑1 (MQ=255)                                                                 aTGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTg                                                                        <  2:894828/140‑1 (MQ=255)                                                                   gactgacCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGaa                                                                      >  1:1000390/1‑140 (MQ=255)                                                                       gacCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAAt                                                                  >  2:538867/1‑140 (MQ=255)                                                                             cgagcgaAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGt                                                            >  1:21435/1‑140 (MQ=255)                                                                              gagcgaAACACTTCCAGAACCATGTAAGAAAACGGTGGCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTa                                                           <  1:489431/140‑1 (MQ=255)                                                                                 cgaAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTAcgc                                                        <  1:388280/140‑1 (MQ=255)                                                                                 cgaAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTAcgc                                                        <  2:537852/140‑1 (MQ=255)                                                                                  gaAACACTCCCGGAACTGGGTCAGAAAACGCTGCCGTCAGACTTTGCATACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTAcgcg                                                       <  2:489077/140‑1 (MQ=255)                                                                                         ttCCGGAACCAGGTCAGAAAATGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTg                                                <  1:868304/140‑1 (MQ=255)                                                                                                aCCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGAt                                         <  2:1000390/140‑1 (MQ=255)                                                                                                     gTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCtc                                    >  2:539636/1‑140 (MQ=255)                                                                                                          aaaaCGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAg                               <  1:705494/140‑1 (MQ=255)                                                                                                          aaaaCGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAg                               >  1:701095/1‑140 (MQ=255)                                                                                                          aaaaCGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAg                               <  1:64813/140‑1 (MQ=255)                                                                                                          aaaaCGGTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAg                               <  2:33031/140‑1 (MQ=255)                                                                                                               ggTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAgg                                            >  2:195890/1‑122 (MQ=255)                                                                                                               ggTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAgg                                            <  1:195890/122‑1 (MQ=255)                                                                                                               ggTGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTc                          >  1:197368/1‑140 (MQ=255)                                                                                                                 tGCCATCATACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCa                        >  2:731721/1‑140 (MQ=255)                                                                                                                     atcatACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATc                    <  1:806835/140‑1 (MQ=255)                                                                                                                         tACTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTtga                >  1:689111/1‑140 (MQ=255)                                                                                                                          aCTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTtgat               <  2:828600/140‑1 (MQ=255)                                                                                                                           cTTTGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGTTTGTAGGTATTCCTCATCAGAGGTCCAGATCTtgatg              >  1:375802/1‑140 (MQ=255)                                                                                                                             ttGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGaa            <  1:726006/140‑1 (MQ=255)                                                                                                                              tGCAAACGTACCGCTTTTTCGGCTAACTGCGTTTCCTACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAAt           <  2:355519/140‑1 (MQ=255)                                                                                                                              tGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAAt           >  2:59889/1‑140 (MQ=255)                                                                                                                              tGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAAt           >  2:555254/1‑140 (MQ=255)                                                                                                                              tGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATAGCTGATCAGATGTGCAGATCTTGATGAAt           >  1:470699/1‑140 (MQ=255)                                                                                                                              tGCAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTCTAGGGATTGCTCATCAGATGTACAGATCTTGATGAAt           >  1:392467/1‑140 (MQ=255)                                                                                                                                cAAACGTACCGCTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGAGATTGCTCATCAGATGTATAGATCTTGATGAATTc         >  2:448001/1‑140 (MQ=255)                                                                                                                                       aCCGCTTTTTCGGCTCACTGCGGTTGATGCTGTTCTGCTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTtg  <  1:285769/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CGAAAATGATGTGATACAGGTCATCGCCATCGTGCCAGATGCGGAACTCCGCGCGCATCCGGTAATGACTGACCGGCGAGCGAAACACTTCCGGAACCAGGTCAGAAAACGGTGCCATCATACTTTGCAAACGTACCACTTTTTCGGCTAACTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTG  >  NC_000913/4163078‑4163351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑