Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,183,531 A→G T763A (ACC→GCC)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,183,5310AG100.0% 204.5 / NA 57T763A (ACC→GCC) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

AAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGA  >  NC_000913/4183399‑4183666
                                                                                                                                    |                                                                                                                                       
aaaCGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATAGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTAt                                                                                                                                  <  1:603010/140‑1 (MQ=255)
      ggtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCa                                                                                                                            <  2:861646/140‑1 (MQ=255)
       gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCAGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAg                                                                                                                           >  2:884695/1‑140 (MQ=255)
        tggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACTACCTGACCTAATACACTCGTTCTAACCAGAACGCCTGTATCAACCAGa                                                                                                                          >  2:329233/1‑140 (MQ=255)
          gtgtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATg                                                                                                                        <  1:921217/140‑1 (MQ=255)
          gtgtCGTTCAGTACGCGGATGCATCCCGTCTCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATg                                                                                                                        <  1:329233/140‑1 (MQ=255)
            gtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGcc                                                                                                                      <  2:858409/140‑1 (MQ=255)
              cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCgt                                                                                                                    <  1:1017175/140‑1 (MQ=255)
                ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCgtgt                                                                                                                  <  2:884606/140‑1 (MQ=255)
                ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAAATGCCgtgt                                                                                                                  <  1:358655/140‑1 (MQ=255)
                     tACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGtc                                                                                                             >  1:231706/1‑140 (MQ=255)
                      aCGTGGATGCTTCCCGTATCGTTATCAAAGTAAACAAAGCCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGtct                                                                                                            <  2:327497/140‑1 (MQ=255)
                          ggATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTgg                                                                                                        >  2:849548/1‑140 (MQ=255)
                               cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa                                                                                                   >  1:824162/1‑140 (MQ=255)
                               cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa                                                                                                   <  2:960441/140‑1 (MQ=255)
                               cTTCCCGTATCGTTATCAAAGTGAACGAAGAAGTGATGTTTCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa                                                                                                   <  2:448524/140‑1 (MQ=255)
                                 tCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAcc                                                                                                 >  2:123776/1‑140 (MQ=255)
                                     gTATCGTTATCAAAGTTAACGACGACGAGATGTATTCGGTTGAAGCAGCTATCAACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGtt                                                                                             <  2:323859/140‑1 (MQ=255)
                                      tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCAACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATTAACCAGATGCCTTGGGTGTCTGTGGGTGAACCGGTTg                                                                                            >  1:323859/1‑140 (MQ=255)
                                                      aaCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCt                                                                            >  1:633761/1‑140 (MQ=255)
                                                             aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGAc                                                                     >  2:824954/1‑140 (MQ=255)
                                                             aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAACGGATGCACGTGGCGACGTGCTGGCAGAc                                                                     >  1:385257/1‑140 (MQ=255)
                                                                   tGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGgtccg                                                               <  1:692203/140‑1 (MQ=255)
                                                                       tCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCa                                                           >  2:931489/1‑140 (MQ=255)
                                                                         cGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCAcc                                                         <  2:852689/140‑1 (MQ=255)
                                                                            gTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGAc                                                      <  1:123776/140‑1 (MQ=255)
                                                                               aaGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTc                                                   >  2:101052/1‑140 (MQ=255)
                                                                               aaGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGAGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGCCGGTCCGTCCACCGACCTc                                                   >  2:210379/1‑140 (MQ=255)
                                                                                       aTCGACATCTACAACCTCACCATATACACCCTTTCTAACTAGAACGCCCGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACt                                           <  2:316570/140‑1 (MQ=255)
                                                                                         cGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTgg                                         <  1:869379/140‑1 (MQ=255)
                                                                                             aTCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGa                                                       >  2:997052/1‑122 (MQ=255)
                                                                                             aTCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGa                                                       <  1:997052/122‑1 (MQ=255)
                                                                                             aTCTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCt                                     <  1:884695/140‑1 (MQ=255)
                                                                                               cTACAACCTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTg                                   >  2:444943/1‑140 (MQ=255)
                                                                                               cTACAACCTGACCAAAATCACCGGTTCTAAATATATCGCTTGTATCAATCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTg                                   <  2:494146/140‑1 (MQ=255)
                                                                                                      cTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGaa                            <  2:231706/140‑1 (MQ=255)
                                                                                                      cTGACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGaa                            >  2:157934/1‑140 (MQ=255)
                                                                                                        gACCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACa                          >  2:225059/1‑140 (MQ=255)
                                                                                                         aCCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACAt                         >  1:904074/1‑140 (MQ=255)
                                                                                                         aCCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACAt                         >  2:876714/1‑140 (MQ=255)
                                                                                                         aCCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACAt                         >  2:786000/1‑140 (MQ=255)
                                                                                                         aCCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACAt                         >  1:635515/1‑140 (MQ=255)
                                                                                                         aCCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGGCCACCGAGCTCTGTGAACTGGAGCTTGGTCAGAACAt                         >  1:274730/1‑140 (MQ=255)
                                                                                                         aCCAAATACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGCCCACCGACCTCGGTCAACTGGCGCTTGGTCAGAACAt                         >  1:392743/1‑140 (MQ=255)
                                                                                                               tACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGt                   >  2:997792/1‑140 (MQ=255)
                                                                                                               tACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGt                   <  1:849548/140‑1 (MQ=255)
                                                                                                               tACACCCGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGt                   <  2:197538/140‑1 (MQ=255)
                                                                                                                   cccGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACa                          >  1:730595/1‑129 (MQ=255)
                                                                                                                   cccGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACa                          <  2:730595/129‑1 (MQ=255)
                                                                                                                     cGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGtt             >  1:57235/1‑140 (MQ=255)
                                                                                                                        tCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCAt          >  2:593563/1‑140 (MQ=255)
                                                                                                                         cTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATg         <  1:444943/140‑1 (MQ=255)
                                                                                                                           aaCCAGAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGcc       <  1:157934/140‑1 (MQ=255)
                                                                                                                                gAACGCCTGTATCAACCAGATGGCGCGTGGGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGa  <  1:345799/140‑1 (MQ=255)
                                                                                                                                gAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGa  <  1:593563/140‑1 (MQ=255)
                                                                                                                                gAACGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGa  <  2:904074/140‑1 (MQ=255)
                                                                                                                                 aaCGCCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGa  <  1:790276/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
AAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGCAGACGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGA  >  NC_000913/4183399‑4183666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑