Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	442,234	A→G	N229S (AAC→AGC)	thiI →	tRNA uridine 4‑sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	442,234	0	A	G	100.0%	116.7 / NA	34	N229S (AAC→AGC)	thiI	tRNA uridine 4‑sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/19);  total (15/19)

TCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAA  >  NC_000913/442101‑442369                                                                                                                                      |                                                                                                                                        tCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCtt                                                                                                                                   <  2:723299/140‑1 (MQ=255)                   ggTGTTTCCAGTTATTTGTTGATGCCTCGTGGCTGCCACGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCgcgt                                                                                                                 <  1:313477/140‑1 (MQ=255)                             gTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTc                                                                                                       <  2:703547/140‑1 (MQ=255)                              ttATATGTTGATGCGTCGCGGCTGCTGCGAGCATTACAGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCg                                                                                                      <  1:338375/140‑1 (MQ=255)                                  atGTTGATGCGTCGCCGCTGCCTCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAt                                                                                                  <  1:320955/140‑1 (MQ=255)                                   tGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAtt                                                                                                 >  2:203760/1‑140 (MQ=255)                                        aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTTGCAGCTCACACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                            >  1:419628/1‑140 (MQ=255)                                        aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTAGCAGCTCACACAGCGTGCGTTGTGTCGCTATTAAttt                                                                                            >  1:293443/1‑140 (MQ=255)                                        aTGCGTCGCGGCTGCCGCGTGCACTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                            >  2:571908/1‑140 (MQ=255)                                            gTCGCGGCTGCCCCGTGCATTACTGCGTCTTTAACCTCGGCGGCTCGGCGCATGTAATTGCCGCGCGCCATGTGGCGCAATGTCTGTTGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                                        >  2:755511/1‑140 (MQ=255)                                             tCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCTGCTTTGGCGGCTTCCATCGCGTGCGTTTTGTAGCTATTAATTTCGAAc                                                                                       >  2:480997/1‑140 (MQ=255)                                                           tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGaa                                                                         >  2:900604/1‑140 (MQ=255)                                                                           tAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTTGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcg                                                         >  2:787745/1‑140 (MQ=255)                                                                            aaCCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTTGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcga                                                        <  1:787745/140‑1 (MQ=255)                                                                                  ggcggggcggCGCATGAAATTAGCGTGCGTAAGGTGGCGCATTATCAATGGATCCTCTATGTGTGCTCCCACCACGTGGGTTTGGTCGCTATTAATTTCGAACCCATCGTCGGGGAAATTCTCGGGAAAAAGGACGACgg                                                  >  2:635517/1‑140 (MQ=255)                                                                                  ggcggcGCGGCGCATTAAATTGACGAGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACgg                                                  <  1:488735/140‑1 (MQ=255)                                                                                  ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACgg                                                  >  1:49175/1‑140 (MQ=255)                                                                                  ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAACGGTCGTCGGGGAAGTTCTCGCGAAAATCGACGACgg                                                  >  1:404375/1‑140 (MQ=255)                                                                                             gcATGAAATTGGCGTGCGTCAGTTGGCGCATTATCTGTGAAGCCGCTTGGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                       <  2:443572/140‑1 (MQ=255)                                                                                             gcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                       <  1:800192/140‑1 (MQ=255)                                                                                             gcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                       <  2:49175/140‑1 (MQ=255)                                                                                             gcATGAAATTGGCGTGCATCAGTTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                       <  2:293443/140‑1 (MQ=255)                                                                                             gcATGAAATTGGCGTCCGTCAGCTGCCGCCTTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                                       <  1:282850/140‑1 (MQ=255)                                                                                               aTGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGtt                                     <  2:801714/140‑1 (MQ=255)                                                                                               aTGAAATTGGCGTGCCTCAGGTCGCCCATCATCTGTGCAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGtt                                     <  1:298941/140‑1 (MQ=255)                                                                                                    aTTGGCGTGCGTCAGGTGGCGCTTTATCTGTGGAGTCGCCTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCt                                <  2:309882/140‑1 (MQ=255)                                                                                                     ttGGCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTc                               <  1:900604/140‑1 (MQ=255)                                                                                                       ggCGTGCGTCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                             <  2:684924/140‑1 (MQ=255)                                                                                                         cgtgcgtCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAc                           <  1:751558/140‑1 (MQ=255)                                                                                                             cgtCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATGGACAACGGTCAGCTGGGCGTTATCCTCAAACGTat                       >  2:348361/1‑140 (MQ=255)                                                                                                             cgtCAGGTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTat                       >  1:878368/1‑140 (MQ=255)                                                                                                                   gTGGCGCATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgt                 >  1:645361/1‑140 (MQ=255)                                                                                                                       cgcATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgt             >  1:597832/1‑140 (MQ=255)                                                                                                                       cgcATTATCTGTGGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgt             >  1:389711/1‑140 (MQ=255)                                                                                                                                 gtgGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTa   <  2:878368/140‑1 (MQ=255)                                                                                                                                  tgGAGCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTaa  >  1:674269/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAA  >  NC_000913/442101‑442369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑