Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,219,224	T→C	V210A (GTA→GCA)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,219,224	0	T	C	100.0%	198.9 / NA	56	V210A (GTA→GCA)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

GCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCT  >  NC_000913/4219087‑4219360                                                                                                                                          |                                                                                                                                         gcgcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAg                                                                                                                                        <  1:97656/140‑1 (MQ=255)   gcTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGc                                                                                                                                      <  1:251012/140‑1 (MQ=255)      ggCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCaaa                                                                                                                                   <  1:555825/140‑1 (MQ=255)      ggCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCaaa                                                                                                                                   <  1:251247/140‑1 (MQ=255)      ggCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCaaa                                                                                                                                   <  1:538175/140‑1 (MQ=255)       gCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAAc                                                                                                                                  <  1:580541/140‑1 (MQ=255)               aaaGCCGTGACATCCATCACATCATTCGCCAGCTGTCGGCAACGCTGGCGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATcaca                                                                                                                          <  2:271639/140‑1 (MQ=255)                 aGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCGATAAAGCGGCCTGGGTGGCAGACAAACTGATCACAcc                                                                                                                        >  1:271639/1‑140 (MQ=255)                    cGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTCGCAGGCAAACTGATCACACCTTc                                                                                                                     >  1:647925/1‑140 (MQ=255)                     gTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTcc                                                                                                                    >  2:501459/1‑140 (MQ=255)                      tGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCg                                                                                                                   >  1:529240/1‑140 (MQ=255)                             cattacattaTTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACAtt                                                                                                            >  2:1017059/1‑140 (MQ=255)                                   attattCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGGTCGCACCTTCCGGCACATTGCCAtt                                                                                                      >  1:373236/1‑140 (MQ=255)                                       ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAGAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                                                                  >  1:1009829/1‑140 (MQ=255)                                       ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCAGACGTTCCGGTACATTGCCATTTTtg                                                                                                  >  1:290883/1‑140 (MQ=255)                                       ttCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTtg                                                                                                  >  2:807212/1‑140 (MQ=255)                                            cATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAAGTGATCACTCCTTCCGGCACATGGCCATTTTTGCTGcc                                                                                             >  1:262160/1‑140 (MQ=255)                                             aTCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACATCTTCCGTCACATTCCCATTTTTGCTGCCg                                                                                            >  1:448360/1‑140 (MQ=255)                                                        aCGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAgac                                                                                 >  1:534985/1‑140 (MQ=255)                                                            tGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGTCACATTGACATATTAGCTGCCGATCCACCAgacggac                                                                             >  1:303843/1‑140 (MQ=255)                                                             ggggACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAgacggacg                                                                            >  1:136214/1‑140 (MQ=255)                                                             ggggACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAgacggacg                                                                            >  1:657517/1‑140 (MQ=255)                                                             ggggACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAgacggacg                                                                            >  1:250920/1‑140 (MQ=255)                                                                 acagacaACCTCGCGCAAAGTCCTTCACAGGTGGCGATCGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACgg                                                                        <  1:300348/140‑1 (MQ=255)                                                                        aCCTCGCGGAAAGTCATCTACATGTGGCGAATGAAATGTCTTACCGCATTAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGtta                                                                 <  2:303843/140‑1 (MQ=255)                                                                         ccTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                                                                <  2:671302/140‑1 (MQ=255)                                                                         ccTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                                                                <  1:807212/140‑1 (MQ=255)                                                                         ccTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                                                                <  1:46178/140‑1 (MQ=255)                                                                          cTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatt                                                               >  2:1007195/1‑140 (MQ=255)                                                                             gcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                                                            >  1:557459/1‑140 (MQ=255)                                                                             gcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                                                            <  2:657517/140‑1 (MQ=255)                                                                             gcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                                                            <  2:250920/140‑1 (MQ=255)                                                                             gcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                                                            <  2:136214/140‑1 (MQ=255)                                                                             gcgGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttatttat                                                            >  1:884947/1‑140 (MQ=255)                                                                               ggAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTg                                                          >  1:148172/1‑140 (MQ=255)                                                                                gAAAGTCATTTACAGATGGCGAACGTACTCTTTTACCTCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGa                                                         <  1:435243/140‑1 (MQ=255)                                                                                 aaaGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                                                                >  2:1007771/1‑132 (MQ=255)                                                                                 aaaGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGttat                                                                <  1:1007771/132‑1 (MQ=255)                                                                                    gTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATAcc                                                     <  2:884947/140‑1 (MQ=255)                                                                                               gTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacgac                                          >  1:126014/1‑140 (MQ=255)                                                                                               gTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacgac                                          <  1:778765/140‑1 (MQ=255)                                                                                                  gCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTgacgacgac                                       >  2:1006478/1‑140 (MQ=255)                                                                                                        gaacTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGa                                 <  2:1000399/140‑1 (MQ=255)                                                                                                         aacTGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACCCCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGaa                                <  2:960095/140‑1 (MQ=255)                                                                                                            tGTTTTACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTAGTCACTGATACCTCCCTGACGACGACCGCCGAAGCg                             >  2:459220/1‑140 (MQ=255)                                                                                                                ttACCGCAATAAAGCCGCCTGGCTGGCAGGCAAACAGATTACACCTTCGGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCa                         <  2:448360/140‑1 (MQ=255)                                                                                                                    cGCAATAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTACTGAGACCAGCCTGACGGCGACGGCCGAAGCGAGCATTGt                     >  2:277029/1‑140 (MQ=255)                                                                                                                        aTAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGtttat                 >  2:29512/1‑138 (MQ=255)                                                                                                                        aTAAAGCCGCCTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGAGACCTGCCGGACGACGAACGCCGAAGCGAGCATTGctttt                 >  2:306029/1‑140 (MQ=255)                                                                                                                             gccgccTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGc              >  2:211774/1‑138 (MQ=255)                                                                                                                             gccgccTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGc              <  1:211774/138‑1 (MQ=255)                                                                                                                                gccTGGCTGGCAGGCCAACTGATCATACCCTCCGGCATATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGGCGACCGCCGAAGCGAGCATTGCTTTTGGCTTTgc         <  1:306029/140‑1 (MQ=255)                                                                                                                                 ccTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTgcg        <  1:1017059/140‑1 (MQ=255)                                                                                                                                 ccTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTgcg        <  1:501459/140‑1 (MQ=255)                                                                                                                                 ccTGGCTGGCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTgcg        >  2:891543/1‑140 (MQ=255)                                                                                                                                 ccTGGCGTGCAGGCAAAATGATCACACCTTCTGGCACATGGCCATTTTCGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATGCCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTgcg        <  2:262160/140‑1 (MQ=255)                                                                                                                                      ctggCAGGCAAACTGCTCACACGTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTc   <  1:459220/140‑1 (MQ=255)                                                                                                                                       tggCAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCt  <  1:636955/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCGCGGAAAGTCATTTACAGGTGGCGAACGAACTGTTTTACCGCAATAAAGCCGCCTGGCTGGTAGGCAAACTGATCACACCTTCCGGCACATTGCCATTTTTGCTGCCGATCCACCAGACGGACGACGGCGAGTTATTTATTGATACCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCT  >  NC_000913/4219087‑4219360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑