Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,224,648	A→G	N274S (AAC→AGC)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,224,648	0	A	G	100.0%	91.0 / NA	26	N274S (AAC→AGC)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

ATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAA  >  NC_000913/4224517‑4224775                                                                                                                                    |                                                                                                                                aTTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGc                                                                                                                         <  2:642167/140‑1 (MQ=255)             aTTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGTCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCt                                                                                                             >  1:917916/1‑140 (MQ=255)               tGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCttt                                                                                                           >  1:536546/1‑140 (MQ=255)                gcgcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTg                                                                                                          <  1:916963/140‑1 (MQ=255)                      cGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGt                                                                                                    >  1:736963/1‑140 (MQ=255)                       gCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCAGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTa                                                                                                   >  2:563199/1‑140 (MQ=255)                         cGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCAGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACg                                                                                                 <  1:563199/140‑1 (MQ=255)                             gCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtct                                                                                             <  1:44889/140‑1 (MQ=255)                                         ggCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGacac                                                                                 <  1:228100/140‑1 (MQ=255)                                         ggCCTGAACTGTGCGCTGGGGCCCGATGAACGGGGGCAGTACGTGCAGTAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGacac                                                                                 <  2:471988/140‑1 (MQ=255)                                               aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGc                                                                           >  1:537480/1‑140 (MQ=255)                                               aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGc                                                                           >  1:91636/1‑140 (MQ=255)                                                   gtgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCaaaa                                                                       <  1:611037/140‑1 (MQ=255)                                                   gtgCGCTGGGGCCCGATAAACTCCGCCAGTACGTGCAGGCGCTGTCACCGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCaaaa                                                                       <  2:371941/140‑1 (MQ=255)                                                    tgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTTCGAGCTCGACGCCCACACGATGGCAAAAc                                                                      >  1:489791/1‑140 (MQ=255)                                                    tgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAAc                                                                      >  1:137809/1‑140 (MQ=255)                                                    tgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAAc                                                                      >  1:512723/1‑140 (MQ=255)                                                               ccGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGaa                                                           <  2:536546/140‑1 (MQ=255)                                                                              aGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt                                            <  2:165899/140‑1 (MQ=255)                                                                              aGTACGTGAAGGAGCTATCACGTATTGCGGACTCCTACGACACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt                                            <  2:489791/140‑1 (MQ=255)                                                                                    tGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGGAAAACAGATACGTGCATGGGCGCAAGCGGGTTTtctc                                      >  1:307798/1‑140 (MQ=255)                                                                                                   ggATTGCGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCggcggc                       <  2:917916/140‑1 (MQ=255)                                                                                                         cGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCtgt                 >  1:136986/1‑140 (MQ=255)                                                                                                         cGGAATGCTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCtgt                 >  2:878040/1‑140 (MQ=255)                                                                                                                cTACGTCACCGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGcacca          <  2:547200/140‑1 (MQ=255)                                                                                                                        ccGCGCACCCGAGCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCCCAATATCCTCGGCGGCTGCTGTGGCACCACGCCacaa  >  1:372186/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                ATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAA  >  NC_000913/4224517‑4224775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑