Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,329,694	T→C	L112P (CTC→CCC)	yjcZ →	uncharacterized protein YjcZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,329,694	0	T	C	100.0%	148.7 / NA	43	L112P (CTC→CCC)	yjcZ	uncharacterized protein YjcZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/27);  total (16/27)

CCTGCGTCTGACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGG  >  NC_000913/4329563‑4329829                                                                                                                                    |                                                                                                                                        ccTGCGTCTGACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGc                                                                                                                                 >  2:190413/1‑140 (MQ=255)      gTCTGACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAAtt                                                                                                                            >  2:923670/1‑140 (MQ=255)      gTCTGACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAAtt                                                                                                                            >  1:894836/1‑140 (MQ=255)      gTCTGACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAAtt                                                                                                                            >  1:82618/1‑140 (MQ=255)      gTCTGACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAAtt                                                                                                                            >  1:614722/1‑140 (MQ=255)          gACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTgccg                                                                                                                        <  1:923670/140‑1 (MQ=255)          gACATTGGTGGAAAAACAGGCGTCGATGCTGCATCCCGGGCATCTGGCCCTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTgccg                                                                                                                        <  2:437623/140‑1 (MQ=255)           aCATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTgccgc                                                                                                                       <  1:712740/140‑1 (MQ=255)            cATTGGTGGAAAAACTGGCGTCCATCCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTgccgcc                                                                                                                      <  1:321193/140‑1 (MQ=255)             aTTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCt                                                                                                                     <  1:856897/140‑1 (MQ=255)                     aaaaaCTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTgccg                                                                                                                        >  2:531275/1‑129 (MQ=255)                     aaaaaCTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTgccg                                                                                                                        <  1:531275/129‑1 (MQ=255)                                   aTGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCg                                                                                               <  1:672971/140‑1 (MQ=255)                                     gCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGtt                                                                                             <  1:938052/140‑1 (MQ=255)                                              cGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTgga                                                                                    >  2:668677/1‑140 (MQ=255)                                              cGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTgga                                                                                    <  2:82618/140‑1 (MQ=255)                                              cGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCATTGTCGCTgga                                                                                    <  1:945420/140‑1 (MQ=255)                                                gTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGGCTTGTATGAGTCGTTTTCAGCGCGTTGTCGCTggaag                                                                                  >  1:369443/1‑140 (MQ=255)                                                gTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTggaag                                                                                  >  1:694855/1‑140 (MQ=255)                                                   aTCTGGCGCTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTggaaggaa                                                                               <  1:557649/140‑1 (MQ=255)                                                            tGACGCAGATCGCGCAGGTTCGGGCGCTGCTTCAACAAAAGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTa                                                                      <  2:329991/140‑1 (MQ=255)                                                                   acaggcgcAGCATCTGGCGCTGGTGCAAAGAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAAc                                                               <  1:413081/136‑1 (MQ=255)                                                                    aTCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACg                                                              <  1:208158/140‑1 (MQ=255)                                                                    aTCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACg                                                              <  1:77951/140‑1 (MQ=255)                                                                        cgcAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAg                                                            >  2:28119/1‑138 (MQ=255)                                                                        cgcAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAg                                                            <  1:28119/138‑1 (MQ=255)                                                                        cgcAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCACGTTGTCACTGGAAGGGAAAGGCGTTAACGCAACGAGGc                                                          >  2:388227/1‑140 (MQ=255)                                                                        cgcAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATAGCCGCCTTGTGTGAGTGGTTTTCAGCCGGTTGGTGTTGGATGGAAAAGGTGTTACTGCAActcggc                                                          >  2:895866/1‑135 (MQ=255)                                                                           ggcaCCTGCCGCTGCTGCAAAAAATGGATCCACGCTAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTa                                                       <  2:419044/139‑1 (MQ=255)                                                                           agcaTCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTa                                                       <  2:694855/140‑1 (MQ=255)                                                                           agcaTCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTa                                                       <  1:172198/140‑1 (MQ=255)                                                                            gcaTCGGGCGCTGCTGCAAAAAATGGACCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTAc                                                      <  2:228188/140‑1 (MQ=255)                                                                             caTCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACt                                                     >  2:954444/1‑140 (MQ=255)                                                                              aTCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTg                                                    >  2:637784/1‑140 (MQ=255)                                                                                cTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGt                                                  <  1:547726/140‑1 (MQ=255)                                                                                  ggCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTGACGCCACGAGGCCTActgctgt                                                >  2:413081/1‑139 (MQ=255)                                                                                   gcgcTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTgca                                               <  2:614722/140‑1 (MQ=255)                                                                                   gcgcTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTgca                                               <  2:894836/140‑1 (MQ=255)                                                                                     gctgctGCAAAAAATGGATCACTGCCAGCACTCTGATTTTCCGGAGCCGCCCCAGCAAATTGCCGCCTGGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTgcagg                                             <  2:369443/140‑1 (MQ=255)                                                                                     gctgctGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTgcagg                                             <  1:668677/140‑1 (MQ=255)                                                                                       tgctgcAAAAAATGGATCACCGTCAGCACTCTGCTTTACCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTgcaggca                                           <  1:388623/140‑1 (MQ=255)                                                                                             aaaaaaTGGATCACCGCCAGCACTCTGCTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGAt                                     <  2:19562/140‑1 (MQ=255)                                                                                                                        cTTTCCCGGAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCGGGCAGGTGATCTGAACGAGCAAATTTTTACCCGCTgg          >  1:321724/1‑140 (MQ=255)                                                                                                                                gAGCCCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTgg  >  2:766916/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        CCTGCGTCTGACATTGGTGGAAAAACTGGCGTCGATGCTGGATCCCGGTCATCTGGCACTGACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGG  >  NC_000913/4329563‑4329829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑