Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,348,226	A→G	D173D (GAT→GAC)	dcuB ←	anaerobic C4‑dicarboxylate transporter DcuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,348,226	0	A	G	100.0%	314.4 / NA	88	D173D (GAT→GAC)	dcuB	anaerobic C4‑dicarboxylate transporter DcuB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/42);  total (46/42)

CATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGACGTTCC  >  NC_000913/4348090‑4348361                                                                                                                                         |                                                                                                                                        cATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCg                                                                                                                                      <  2:854612/140‑1 (MQ=255)   tACTCACGGTTTTCCGGTACGGCGATGAAGTTCTGGACCTCTTTGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAg                                                                                                                                    <  1:400722/140‑1 (MQ=255)   tACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAg                                                                                                                                    <  2:749496/140‑1 (MQ=255)   tACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAg                                                                                                                                    <  1:936316/140‑1 (MQ=255)   tACTCACGGTTTTCCGCTACGGCGATGAATCGCAGTAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCAATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAg                                                                                                                                    <  2:485786/140‑1 (MQ=255)     cTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGa                                                                                                                                  >  1:656372/1‑140 (MQ=255)     cTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGa                                                                                                                                  >  1:508700/1‑140 (MQ=255)     cTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGa                                                                                                                                  >  1:668353/1‑140 (MQ=255)     cTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATAGTGATTCCCAGCAGGTCGAGGa                                                                                                                                  >  1:463407/1‑140 (MQ=255)     cTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTGTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGa                                                                                                                                  >  2:614139/1‑140 (MQ=255)         cGGTTTTCCGGTACGGAGATGAATTTCTGGAACTTTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTc                                                                                                                              <  1:1005891/140‑1 (MQ=255)         cGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACATCGATGGAATGGTGCTTGCCAACAGGTCGAGGAACTc                                                                                                                              >  1:435527/1‑140 (MQ=255)          ggTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAg                                                                                                                                    <  2:80068/133‑1 (MQ=255)          ggTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAg                                                                                                                                    >  1:80068/1‑133 (MQ=255)          ggTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCa                                                                                                                             <  1:561182/140‑1 (MQ=255)          ggTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCa                                                                                                                             >  2:203308/1‑140 (MQ=255)            ttttCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATTGTGATTACCAGCATGTCGAGGAACTCAAg                                                                                                                           >  1:324160/1‑140 (MQ=255)               tCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATg                                                                                                                        >  2:143598/1‑140 (MQ=255)                       ggAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCAt                                                                                                                <  1:22230/140‑1 (MQ=255)                        gagaTGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATc                                                                                                               <  2:656372/140‑1 (MQ=255)                             gAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAgg                                                                                                          <  2:1011252/140‑1 (MQ=255)                             gAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAgg                                                                                                          <  1:151303/140‑1 (MQ=255)                                  tCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACa                                                                                                     <  2:668353/140‑1 (MQ=255)                                   cTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACAt                                                                                                    >  1:740707/1‑140 (MQ=255)                                     ggAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATGGCCAGCAGGTCGCGGAACTCAAGATAGCGAGCATCAAAGGTGACATTa                                                                                                  >  2:278194/1‑140 (MQ=255)                                        aCTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTAccc                                                                                               <  1:252461/140‑1 (MQ=255)                                           ctTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGc                                                                                            >  2:207711/1‑140 (MQ=255)                                               gTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCg                                                                                        <  2:969270/140‑1 (MQ=255)                                                    tATCCAGATCTTTACCGCGGAACCACCTGTAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAAcc                                                                                   <  1:324989/140‑1 (MQ=255)                                                      tCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCag                                                                                 >  2:669916/1‑140 (MQ=255)                                                           aTCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGaca                                                                            >  1:525058/1‑140 (MQ=255)                                                           aTCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGaca                                                                            >  2:850528/1‑140 (MQ=255)                                                           aTCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGaca                                                                            <  1:669916/140‑1 (MQ=255)                                                           aTCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGaca                                                                            <  1:207711/140‑1 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  1:1003673/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  1:574012/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  2:916253/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  2:758770/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  1:12957/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  2:733739/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  2:147905/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGAcc                                                                       >  1:836726/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGACGACCATCAAAGCTGAGATTACCCATCATCTCAACCAGAGACACGAcc                                                                       >  2:291350/1‑140 (MQ=255)                                                                tACCGCGGAACCAGCTGAAGATACCGATCGCCAGG‑‑‑‑‑GATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAAc                                                                  >  1:533253/1‑140 (MQ=255)                                                                     cgGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAAc                                                                  >  2:514002/1‑140 (MQ=255)                                                                     cgGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAAc                                                                  <  1:553153/140‑1 (MQ=255)                                                                       gAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACATGACCGCAACCg                                                                >  2:67762/1‑140 (MQ=255)                                                                       gAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCg                                                                >  1:201333/1‑140 (MQ=255)                                                                       gAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATGAACCAGCATCGCGACCAGAGACACGACCGCAACCg                                                                >  2:448873/1‑140 (MQ=255)                                                                       gAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCAGCAAAGGTGACATAACCAAGCATCGCCACCACAGACACGACCGCAACCg                                                                >  2:332920/1‑140 (MQ=255)                                                                           cAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGacac                                                            <  2:508700/140‑1 (MQ=255)                                                                             gCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTAGCCAGCATGGCAACCAGGGACATGACCGCAACCGACACCg                                                          >  2:259755/1‑140 (MQ=255)                                                                               tGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCTAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGa                                                        <  1:676890/140‑1 (MQ=255)                                                                               tGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGa                                                        <  1:614139/140‑1 (MQ=255)                                                                                      aCCGATCGCCAGGATTCCGATTAACGTCGTTGGAATGCTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 <  1:448873/140‑1 (MQ=255)                                                                                      aCCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 <  1:203308/140‑1 (MQ=255)                                                                                      aCCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 >  2:722484/1‑140 (MQ=255)                                                                                      aCCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 <  1:722484/140‑1 (MQ=255)                                                                                      aCCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 <  1:143598/140‑1 (MQ=255)                                                                                      aCCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 <  2:12957/140‑1 (MQ=255)                                                                                      aCCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 <  1:850528/140‑1 (MQ=255)                                                                                      aCCGATCGCCAGGATACAGATTCACGTCGATCGAAGGGTTATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGa                                                 <  2:406138/140‑1 (MQ=255)                                                                                        cGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATa                                               >  1:844133/1‑140 (MQ=255)                                                                                          aTCGCCAGGATACCGGTTAACGTCGCTGGTATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAAt                                             <  1:332920/140‑1 (MQ=255)                                                                                            cGCCAGGATACCGATTAACGTAGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGTGACACGAGCGCGACAGACACCAGACTGGCGATAATcc                                           >  1:327939/1‑140 (MQ=255)                                                                                             gCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATccc                                          <  1:230853/140‑1 (MQ=255)                                                                                              ccAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAAGCGACACCGGTCTGGCGATAATcccc                                         >  1:456379/1‑140 (MQ=255)                                                                                               cAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCa                                        >  1:126344/1‑140 (MQ=255)                                                                                                aGGATACCGATTAACGGCGATGCAATGGTGACTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCAt                                       <  2:435527/140‑1 (MQ=255)                                                                                                     aCCGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACATGACCGCAACCGACACCGGACTGGCGATAATCCCCATCtgtg                                  <  1:67762/140‑1 (MQ=255)                                                                                                      gggATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGc                                 <  2:533253/138‑1 (MQ=255)                                                                                                      ccGATTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGc                                 <  2:836726/140‑1 (MQ=255)                                                                                                       cGATTAACGTAGATGGAGTGGTGATTGCCAGAAGGGCGACGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCa                                <  2:327939/140‑1 (MQ=255)                                                                                                         aTTAACGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGAAACAGCCACCGGACTGACGATAATCCCCATATGTGCAcc                              >  2:478733/1‑140 (MQ=255)                                                                                                            aaCGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGAt                           >  1:1005309/1‑140 (MQ=255)                                                                                                            aaCGTCGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGAt                           >  2:703355/1‑140 (MQ=255)                                                                                                                 cGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAAc                      >  2:878036/1‑140 (MQ=255)                                                                                                                 cGATGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAAc                      >  1:739735/1‑140 (MQ=255)                                                                                                                   aTGGAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACtt                    >  1:796513/1‑140 (MQ=255)                                                                                                                    tGGAATGGTGATTGCAAGCAGGCCGCGGACCTCAAGACGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTg                   <  1:291350/140‑1 (MQ=255)                                                                                                                     ggAATGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTgc                  <  2:740707/140‑1 (MQ=255)                                                                                                                       aaTGGTGATTGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTgccg                >  1:37765/1‑140 (MQ=255)                                                                                                                                tGCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGAc       <  2:525058/140‑1 (MQ=255)                                                                                                                                 gCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGACg      >  1:734249/1‑140 (MQ=255)                                                                                                                                 gCCAGCAGGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGACg      >  2:713593/1‑140 (MQ=255)                                                                                                                                   cagcagGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGACGtt    <  2:1003673/140‑1 (MQ=255)                                                                                                                                    agcagGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATTATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGACGTTc   <  1:758770/140‑1 (MQ=255)                                                                                                                                    agcagGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGACGTTc   <  1:703355/140‑1 (MQ=255)                                                                                                                                     gcagGTCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCAACTGTGCACCGAGAGGACTTGCCGCCATCGGACGTTcc  >  1:495128/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CATACTCACGGTTTTCCGGTACGGAGATGAATTTCTGGAACTCTTCGTCTTTATCCAGATCTTTACCGCGGAACCAGCTGAAGATACCGATCGCCAGGATACCGATTAACGTCGATGGAATGGTGATTGCCAGCAGATCGAGGAACTCAAGATGGCGACCATCAAAGGTGACATTACCCAGCATCGCAACCAGAGACACGACCGCAACCGACACCGGACTGGCGATAATCCCCATCTGTGCACCGATAGAACTTGCCGCCATCGGACGTTCC  >  NC_000913/4348090‑4348361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑