| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 445,815 | G→A | C284C (TGC→TGT) | yajR ← | putative major facilitator superfamily transport protein YajR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 445,815 | 0 | G | A | 96.5% | 178.5 / ‑2.4 | 57 | C284C (TGC→TGT) | yajR | putative major facilitator superfamily transport protein YajR |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (26/29); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCA > NC_000913/445678‑445951 | tctTTACTGATAAGTTAGGGCATGATGGCATCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGAcaca < 1:347205/140‑1 (MQ=255) aGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGAc < 2:1012847/140‑1 (MQ=255) aGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGAc > 2:521360/1‑140 (MQ=255) tGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACtt > 1:961529/1‑140 (MQ=255) tGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACtt > 1:795516/1‑140 (MQ=255) tGAGGGCAGGAGGGCTACCATCAAATTAAACCCGAGAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACtt < 2:449274/140‑1 (MQ=255) aGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGc > 2:86900/1‑140 (MQ=255) gggCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCt > 1:795359/1‑140 (MQ=255) gggCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCt > 2:765275/1‑140 (MQ=255) gggCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCt > 2:154413/1‑140 (MQ=255) gaggGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCAttt > 1:501155/1‑140 (MQ=255) gaggGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACAATCAACCAGACACAGAAGACATAGACTTGCTTCAttt > 1:375062/1‑140 (MQ=255) gggCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTg < 1:1012975/140‑1 (MQ=255) gggCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTg < 2:854850/140‑1 (MQ=255) cTTCCATCAAATTAATCGCCACCAAGAAATGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTgcgc < 1:500477/140‑1 (MQ=255) cTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCCACCACGATAAACGCAAGACAGAAGATAAAGAGTTGCTTCGTTTTgcgc > 2:491381/1‑140 (MQ=255) cTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTgcgc > 2:674209/1‑140 (MQ=255) ttCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCt > 1:603272/1‑140 (MQ=255) aTCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAAc > 1:543074/1‑140 (MQ=255) tCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACt > 1:39678/1‑140 (MQ=255) tCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACt < 1:251875/140‑1 (MQ=255) ttAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGc < 2:25788/140‑1 (MQ=255) tAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCg < 2:695382/140‑1 (MQ=255) aaCGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACt > 1:940301/1‑132 (MQ=255) aaCGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAACACTTGCTTCATTTTGCGCTTAACt < 2:940301/132‑1 (MQ=255) aCGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAg < 2:961529/140‑1 (MQ=255) aCGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAg > 1:227625/1‑140 (MQ=255) caAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATg < 2:603272/140‑1 (MQ=255) caAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATg > 2:18579/1‑140 (MQ=255) aaaGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGc < 1:698563/140‑1 (MQ=255) aGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTACATATTGTGCTTAACTGCAGCGTAGATAATGAAAGGCAc > 2:287159/1‑140 (MQ=255) tGCACGCCGACCACCAGTTGCCTGATCTGAGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGAc < 1:287159/140‑1 (MQ=255) cGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATg > 1:244516/1‑125 (MQ=255) cGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATg < 2:244516/125‑1 (MQ=255) ccaccaGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAg < 1:212605/140‑1 (MQ=255) caGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGa > 1:754724/1‑140 (MQ=255) gCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGc > 1:596461/1‑140 (MQ=255) cAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCAt < 1:765275/140‑1 (MQ=255) aaCTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGt > 2:546247/1‑140 (MQ=255) aCTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTc < 2:501155/140‑1 (MQ=255) cTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCg < 1:18579/140‑1 (MQ=255) cTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCg < 2:596461/140‑1 (MQ=255) gTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAg > 2:161959/1‑140 (MQ=255) ttGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGAt < 1:86900/140‑1 (MQ=255) tCCACAACACAATTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTc < 2:227625/140‑1 (MQ=255) aaTTTCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAg < 2:795359/140‑1 (MQ=255) tttCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAgcc > 1:172143/1‑140 (MQ=255) tttCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAgcc > 1:510649/1‑140 (MQ=255) tCCGCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAgccgc > 1:227704/1‑140 (MQ=255) gCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCgg < 2:524438/140‑1 (MQ=255) gCAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCgg < 1:546247/140‑1 (MQ=255) cAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGTCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCggg < 1:570226/140‑1 (MQ=255) cAACCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCggg < 1:674209/140‑1 (MQ=255) aCCACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGaa < 2:754724/140‑1 (MQ=255) cACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAAcc < 2:510649/140‑1 (MQ=255) cACGATCAACCCGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAAcc < 1:521360/140‑1 (MQ=255) cccGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAg > 2:598075/1‑140 (MQ=255) cccGACACAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAg > 2:823208/1‑140 (MQ=255) gACACAGAAGACAAAAACTTGCTTCCTTTCGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCa < 1:500723/140‑1 (MQ=255) gACACAGAAGACAAAAACTTGCTTCATTTGGCGCTTACCTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCa < 1:491381/140‑1 (MQ=255) | TCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCGCGTTCCACAACACAATTTCCGCAACCACGATCAACCCGACGCAGAAGACAAAGACTTGCTTCATTTTGCGCTTAACTTCAGCGTAGATAATGAAAGGCACGACCGAGCCAAAGGCGATTAGCATTGTCGCCAGATAGACCTTCCAGTGTTCAGCCGCCGGGAACCCTGCATCAGCCA > NC_000913/445678‑445951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |