Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,322	C→T	A419A (GCC→GCT)	nnr →	NAD(P)HX epimerase/NAD(P)HX dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,322	0	C	T	100.0%	134.6 / NA	43	A419A (GCC→GCT)	nnr	NAD(P)HX epimerase/NAD(P)HX dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/29);  total (14/29)

CGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGT  >  NC_000913/4395186‑4395454                                                                                                                                         |                                                                                                                                     cgcATCCTGGCGAGGCCGCACGGTTCTTAAGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCgctgcc                                                                                                                                   <  1:498448/140‑1 (MQ=255)     tCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATc                                                                                                                               >  1:789217/1‑140 (MQ=255)      ccTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATcc                                                                                                                              >  2:160785/1‑140 (MQ=255)        tGGCGAGGCCGCACGCTTGTTAAGCCGTTTCGTCGCTAAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTCCAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTg                                                                                                                            <  1:498062/140‑1 (MQ=255)         ggCGAGGCCGCACGGTTGTTAGTCTGTTCCGGCGCAGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGa                                                                                                                           <  2:419866/140‑1 (MQ=255)         ggCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGa                                                                                                                           <  2:789217/140‑1 (MQ=255)          gCGAGGCCGCACGGTTCTTAGGATGTTCCGTAGTTTAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGAc                                                                                                                          <  2:460656/140‑1 (MQ=255)              ggCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCtt                                                                                                                      >  2:789167/1‑140 (MQ=255)               gCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCttt                                                                                                                     <  2:582546/140‑1 (MQ=255)               gCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCttt                                                                                                                     <  2:848266/140‑1 (MQ=255)               gCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCttt                                                                                                                     <  2:100748/140‑1 (MQ=255)               gCCGCACGGTTGTTAAGCTGTTCCGACGCAGAAATTGGAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCttt                                                                                                                     <  1:296310/140‑1 (MQ=255)                   cACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGGACTGTCAGGTGCCGGCACCGGGGTCACAGCCCATCCTGACCCttcacgg                                                                                                                 >  2:976958/1‑135 (MQ=255)                       gttgttAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCatta                                                                                                             <  1:900509/140‑1 (MQ=255)                                cTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCg                                                                                                    <  2:526775/140‑1 (MQ=255)                                 tGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCgg                                                                                                   <  1:188639/140‑1 (MQ=255)                                  gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGa                                                                                                  >  1:642444/1‑140 (MQ=255)                                  gTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGa                                                                                                  >  2:612096/1‑140 (MQ=255)                                      cGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATg                                                                                              <  1:234098/140‑1 (MQ=255)                                             gAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCAt                                                                                       >  2:765165/1‑140 (MQ=255)                                                           ccGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACCCTTTAGGCCTTAATGATGCCGGAAAAGCAGTCATGGCGAGCGGCGGCa                                                                         >  2:371677/1‑140 (MQ=255)                                                                 aCATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCg                                                                   <  1:97469/140‑1 (MQ=255)                                                                     tGCGCCAAACGTCTGGTACAACGTTGTGGGGGCGTAGGGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGAtgt                                                               <  1:371677/140‑1 (MQ=255)                                                                      gcgcCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGAtgtg                                                              <  2:251528/140‑1 (MQ=255)                                                                      gcgcCAAACGTCTGGTACAACGTTACGGCGGCGTAGCTGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAAAGCAGGCATGGCGAGCGGCGGCATGGGCGAtgtg                                                              <  1:408376/140‑1 (MQ=255)                                                                        gcCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGct                                                            <  2:768530/140‑1 (MQ=255)                                                                         ccAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGctc                                                           <  1:612096/140‑1 (MQ=255)                                                                         ccAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGctc                                                           <  1:256968/140‑1 (MQ=255)                                                                         ccAAACGTCTGGTACAACGTTACGGCGGCGTAGCGCTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGctc                                                           <  2:263571/140‑1 (MQ=255)                                                                          cAAACGTCTGGTACACCGTTATTGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGctct                                                          <  1:369382/140‑1 (MQ=255)                                                                            aaCGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGGGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGACGCCGGAAACGCAGGCAAGGCGGGCGTCGGCGTGGGCGATGTGctctct                                                        >  2:369382/1‑140 (MQ=255)                                                                               gTCTGGTACAACGTTATGGCGGCGTAGCGGTCCTGAAAGGTGCCGGAAACGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGt                                                     <  2:359676/140‑1 (MQ=255)                                                                                 cTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCCTGGCGAGCGGCCGCATGGGCGCTGTGCTCTCTGGtat                                                   >  1:319292/1‑140 (MQ=255)                                                                                 cTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGtat                                                   >  2:36946/1‑140 (MQ=255)                                                                                    gTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGtattat                                                <  2:110991/140‑1 (MQ=255)                                                                                    gTACAACGTCATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGtattat                                                <  2:592018/140‑1 (MQ=255)                                                                                          cGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCAGCGGCATAGGCTATATGCTCTGTGGTATTATTGgcgc                                          >  1:296350/1‑140 (MQ=255)                                                                                              aTGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                      >  2:818923/1‑140 (MQ=255)                                                                                               tGGCGGCGTAGCGGTGCTGAAAGGGGTCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCCTTGc                                     >  1:1010789/1‑140 (MQ=255)                                                                                                ggcggcGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                    <  1:160785/140‑1 (MQ=255)                                                                                                                tGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                    <  2:642444/140‑1 (MQ=255)                                                                                                                tGAAAGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                    <  1:907450/140‑1 (MQ=255)                                                                                                                  aaaGGTGCCGGAACCGTGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGcc                  >  1:916148/1‑140 (MQ=255)                                                                                                                    aGGTGCCGGAACCGTGGTCGCTTCCCATCCTAACGCTTTTGGCATTATCGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGt                <  2:296350/140‑1 (MQ=255)                                                                                                                                  tGGTCGCTGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtgt  <  1:789167/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCATCCTGGCGAGGCCGCACGGTTGTTAGGCTGTTCCGTCGCTGAAATTGAAAGTGACCGCTTACATTGCGCCAAACGTCTGGTACAACGTTATGGCGGCGTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGT  >  NC_000913/4395186‑4395454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑