Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,036	C→T	A110V (GCC→GTC)	ulaC →	L‑ascorbate specific PTS enzyme IIA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,036	0	C	T	100.0%	201.3 / NA	62	A110V (GCC→GTC)	ulaC	L‑ascorbate specific PTS enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/25);  total (37/25)

GGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAACGCGGCAGCTTAA  >  NC_000913/4421899‑4422172                                                                                                                                          |                                                                                                                                         ggCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCa                                                                                                                                        >  2:190769/1‑140 (MQ=255)  gCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCaa                                                                                                                                       >  2:748075/1‑140 (MQ=255)   cAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTACCGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCGCATCACCATGGCGGCGGTCGATGTCAAt                                                                                                                                      <  1:962923/140‑1 (MQ=255)     aTGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATAc                                                                                                                                    <  2:155936/140‑1 (MQ=255)     aTGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATAc                                                                                                                                    >  1:902999/1‑140 (MQ=255)     aTGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATAc                                                                                                                                    <  2:805268/140‑1 (MQ=255)      tGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACt                                                                                                                                   <  1:748075/140‑1 (MQ=255)         cGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCCCACTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCAc                                                                                                                                <  2:715081/140‑1 (MQ=255)              gggCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGa                                                                                                                           <  2:902999/140‑1 (MQ=255)                   tCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATATTCACCAGGAAGTgg                                                                                                                      <  2:766874/140‑1 (MQ=255)                   tCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTgg                                                                                                                      >  1:945333/1‑140 (MQ=255)                   tCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCAAGGCAGCGGTCGATGTCAATACTCACCAGGAAGTgg                                                                                                                      >  2:332604/1‑140 (MQ=255)                            gggCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGc                                                                                                             <  1:221133/140‑1 (MQ=255)                             ggCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCa                                                                                                            >  2:644779/1‑140 (MQ=255)                             ggCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCa                                                                                                            >  1:93094/1‑140 (MQ=255)                             ggCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCa                                                                                                            >  1:198634/1‑140 (MQ=255)                               cGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGa                                                                                                          >  2:186829/1‑140 (MQ=255)                               cGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGa                                                                                                          >  2:814270/1‑140 (MQ=255)                               cGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGGCGTTTTCAATACTCAGCAGGTTGTGGGCATCATGCAGa                                                                                                          >  2:436077/1‑140 (MQ=255)                               cGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGCTCGATATGAATACTCAACAGGGCGTGGGCATCATGCAGa                                                                                                          >  2:500621/1‑140 (MQ=255)                                gTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGAt                                                                                                         >  2:621968/1‑140 (MQ=255)                                       aaaCCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAAc                                                                                                  <  1:644779/140‑1 (MQ=255)                                       aaaCCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAAc                                                                                                  <  2:198634/140‑1 (MQ=255)                                       aaaCCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAAc                                                                                                  <  1:190769/140‑1 (MQ=255)                                             gTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGttt                                                                                            >  1:247350/1‑140 (MQ=255)                                                    tctGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTtgaagatg                                                                                     >  2:197219/1‑140 (MQ=255)                                                     ctGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTtgaagatga                                                                                    >  2:566403/1‑140 (MQ=255)                                                       ggTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTtgaagatgaag                                                                                  <  2:247350/140‑1 (MQ=255)                                                       ggTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTtgaagatgaag                                                                                  <  1:186829/140‑1 (MQ=255)                                                       ggTAACGCTGAAAAAACCGCTGTAGCTCAGCCACGATCACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTtgaagatgaag                                                                                  <  1:500621/140‑1 (MQ=255)                                                                gAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGCCAACCTATTTGAAGATGAAGAGAATTTTg                                                                         >  1:375888/1‑140 (MQ=255)                                                                   aaaGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGAcc                                                                      <  2:749553/140‑1 (MQ=255)                                                                           tGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGGTGCAGAGAATTTTGACCGTTTAcga                                                              >  1:403083/1‑139 (MQ=255)                                                                                 tCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGc                                                        >  1:134964/1‑140 (MQ=255)                                                                                     ccACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTa                                                    <  1:621968/140‑1 (MQ=255)                                                                                     ccACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTa                                                    <  1:197219/140‑1 (MQ=255)                                                                                       aCGATGACAACGATCCGGTGGATATCCTCATTACCTTGGGGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGAGCCTGCCGTAcc                                                  <  2:403083/140‑1 (MQ=255)                                                                                               aaCGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGa                                          <  1:887859/140‑1 (MQ=255)                                                                                                   aTCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCGATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTa                                      >  2:938325/1‑140 (MQ=255)                                                                                                   aTCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAgaa                                      >  2:808114/1‑138 (MQ=255)                                                                                                       ggTGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTgg                                  >  2:785617/1‑140 (MQ=255)                                                                                                         tGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTa                                      >  2:717508/1‑134 (MQ=255)                                                                                                         tGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTa                                      <  1:717508/134‑1 (MQ=255)                                                                                                         tGGATATCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGAt                                >  2:169759/1‑140 (MQ=255)                                                                                                            atatCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAgg                                           <  2:978851/126‑1 (MQ=255)                                                                                                            atatCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAgg                                           >  1:978851/1‑126 (MQ=255)                                                                                                            atatCCTCATCACCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGAtctc                             >  2:524021/1‑140 (MQ=255)                                                                                                                  tcatcaCCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGAc                       <  1:566403/140‑1 (MQ=255)                                                                                                                      caCCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGACGTACCGGGCAAGAGGTACTGAATCTCATTGACCGCa                   >  1:379033/1‑140 (MQ=255)                                                                                                                       aCCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCAc                  >  2:817011/1‑140 (MQ=255)                                                                                                                       aCCATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCAc                  >  1:738230/1‑140 (MQ=255)                                                                                                                        ccATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCAcc                 <  2:134964/140‑1 (MQ=255)                                                                                                                         cATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTAGGCGCCTGCCGTACCGACCAGGAAGTGCTGGATCTCATTGACCGCACCa                >  2:292352/1‑140 (MQ=255)                                                                                                                         cATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCa                >  2:217265/1‑140 (MQ=255)                                                                                                                         cATGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTAAGCGCCTGCTGTACCGAACAGGAAGTCCTGGATCTCATTGACCGCACCa                >  2:401783/1‑140 (MQ=255)                                                                                                                           tGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAAc              >  2:82809/1‑140 (MQ=255)                                                                                                                           tGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAAc              >  2:846870/1‑140 (MQ=255)                                                                                                                           tGGCGGCGGTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAAc              >  1:168053/1‑140 (MQ=255)                                                                                                                              cggcggTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAAcgcg           <  1:846870/140‑1 (MQ=255)                                                                                                                                 cggTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAACGCGGCa        >  1:984322/1‑140 (MQ=255)                                                                                                                                   gTCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATGTCGTTGACCTCACCAACGCGGCAGc      >  1:296411/1‑140 (MQ=255)                                                                                                                                    tCGATGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAACGCGGCAGCt     <  1:82809/140‑1 (MQ=255)                                                                                                                                       aTGTCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAACGCGGCAGCTTaa  >  1:230254/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         GGCAATGCCGCACGGGCGTCCGGAAGAGGGCGTTAAGAAAACCGGTTTCTCTCTGGTAACGCTGAAAAAGCCGCTGGAGTTCAACCACGATGACAACGATCCGGTGGATATCCTCATCACCATGGCGGCGGTCGATGCCAATACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGATGAAGAGAATTTTGACCGTTTACGCGCCTGCCGTACCGAGCAGGAAGTACTGGATCTCATTGACCGCACCAACGCGGCAGCTTAA  >  NC_000913/4421899‑4422172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑