Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,440,503	A→G	V238A (GTG→GCG)	ytfL ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,440,503	0	A	G	100.0%	204.2 / NA	56	V238A (GTG→GCG)	ytfL	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/23);  total (33/23)

CGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTAC  >  NC_000913/4440378‑4440635                                                                                                                              |                                                                                                                                     cgcTGTTCAGTGCCATGCTTTGGTTAGCCGGCACGCGTTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAgg                                                                                                                        <  1:430898/140‑1 (MQ=255)   cTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCt                                                                                                                      <  2:178811/140‑1 (MQ=255)        cAGTGCCAGGCTTTGGTTAGCCAGCTCGCGGTTCAGCGGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCt                                                                                                                 <  1:400397/140‑1 (MQ=255)             ccAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCt                                                                                                            >  2:917250/1‑140 (MQ=255)             ccAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCt                                                                                                            <  2:745615/140‑1 (MQ=255)                ggCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCg                                                                                                         >  2:956320/1‑140 (MQ=255)                  cTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTg                                                                                                       <  2:751548/140‑1 (MQ=255)                     tGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCt                                                                                                                 >  2:734735/1‑127 (MQ=255)                     tGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCt                                                                                                                 <  1:734735/127‑1 (MQ=255)                     tGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTg                                                                                                       <  2:127460/137‑1 (MQ=255)                     tGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTg                                                                                                       >  1:127460/1‑137 (MQ=255)                     tGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGgag                                                                                                    >  2:223582/1‑140 (MQ=255)                                cACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCaaa                                                                                         >  1:60710/1‑140 (MQ=255)                                   gcgGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCa                                                                                      <  2:42692/140‑1 (MQ=255)                                               gTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCAcgcg                                                                          >  2:239460/1‑140 (MQ=255)                                               gTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCAcgcg                                                                          <  1:956320/140‑1 (MQ=255)                                               gTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCAcgcg                                                                          <  1:917250/140‑1 (MQ=255)                                                 cTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGgt                                                                        >  1:760398/1‑140 (MQ=255)                                                         tCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTGATCTTCGTCGAGATCAAACCAAATCACGTTCGCACGCGGTGTCATTGa                                                                >  2:498957/1‑140 (MQ=255)                                                         tCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGa                                                                >  2:117124/1‑140 (MQ=255)                                                                     atgatgTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGt                                                    >  1:41051/1‑140 (MQ=255)                                                                     atgatgTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAAATCAAACCTAATAACGGTTTCACGCTGTGTTATTGAAGACGGAACGGt                                                    >  2:270527/1‑140 (MQ=255)                                                                      tgatgTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTa                                                   <  1:849299/140‑1 (MQ=255)                                                                       gatgTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTAc                                                  >  1:1010015/1‑140 (MQ=255)                                                                            ggTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACTTTTACACTCGGTGTCACTGAAGACGGAACGGTACGGGAt                                             >  2:289137/1‑140 (MQ=255)                                                                             gTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGAtt                                            >  2:607285/1‑140 (MQ=255)                                                                              tCAATATCTTCATTAGAGACGACGACCTTTGAGTGCGTATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTc                                           <  1:270527/140‑1 (MQ=255)                                                                                       tCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCaaa                                  <  2:942838/140‑1 (MQ=255)                                                                                            aCAGACGAGGAACTTAGAGTGCGTATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGCACGGGATTCCAGCTCAAAGACGt                             >  2:559953/1‑140 (MQ=255)                                                                                              aGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGttt                           >  1:158937/1‑140 (MQ=255)                                                                                              aGACGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGttt                           >  2:544160/1‑140 (MQ=255)                                                                                                aCGAGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTc                         >  1:605094/1‑140 (MQ=255)                                                                                                   aGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAAt                      >  2:939461/1‑140 (MQ=255)                                                                                                   aGGAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACCGAACGGTACGGCATTCCAGCTTAAAGACGTTTTCAAt                      >  1:491021/1‑140 (MQ=255)                                                                                                     gAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACTGAACGGTACGGGATTCGAGCTAAAAGACGTTTTCAATCa                    >  2:289503/1‑140 (MQ=255)                                                                                                     gAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCa                    >  1:68124/1‑140 (MQ=255)                                                                                                     gAACTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCa                    >  1:862336/1‑140 (MQ=255)                                                                                                       aCTTAGAGTGCGGATTTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                  <  1:408207/140‑1 (MQ=255)                                                                                                       aCTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                  >  2:891473/1‑140 (MQ=255)                                                                                                       aCTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                  <  2:224121/140‑1 (MQ=255)                                                                                                        cTTAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCt                 <  2:219534/140‑1 (MQ=255)                                                                                                         ttAGAGTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTc                <  1:239460/140‑1 (MQ=255)                                                                                                           agagTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCgt              >  1:218995/1‑140 (MQ=255)                                                                                                           agagTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCgt              <  1:607285/140‑1 (MQ=255)                                                                                                              gTGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTGGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGtt           <  2:22594/140‑1 (MQ=255)                                                                                                               tGCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACTGGAGTCCAGCTCAAAGTCGTTGTCAATCAGCTCGTGTTc          >  2:259307/1‑140 (MQ=255)                                                                                                                gCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGCTTCCAGCCCAACGATGTTTTCAATCAGCTCGTGTTcc         >  1:344812/1‑140 (MQ=255)                                                                                                                gCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGtt           <  2:593747/138‑1 (MQ=255)                                                                                                                gCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGtt           >  1:593747/1‑138 (MQ=255)                                                                                                                gCGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGCGATCCCAACTCAAAGAGGTTTACAATCAGCTCGTGTTcc         >  2:408207/1‑140 (MQ=255)                                                                                                                 cGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCt        <  2:158937/140‑1 (MQ=255)                                                                                                                 cGGATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCt        >  1:624557/1‑140 (MQ=255)                                                                                                                  ggATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                  <  2:1020035/129‑1 (MQ=255)                                                                                                                  ggATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGc                  >  1:1020035/1‑129 (MQ=255)                                                                                                                   gATGTTCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGt      <  1:117124/140‑1 (MQ=255)                                                                                                                       ttCCGCCGCCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTAc  >  2:708529/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                     CGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCTTTAGAATCGACATAACCGATGATGTGGTCAATATCTTCATTACAGACGAGGAACTTAGAGTGCGGATGTTCCGCCACCTTATTCTTCAGGCTTTGCTCATCTTCGTGGAGATCAAACCAAATCACGTTTTCACGCGGTGTCATTGAAGACGGAACGGTACGGGATTCCAGCTCAAAGACGTTTTCAATCAGCTCGTGTTCCTGTTTAC  >  NC_000913/4440378‑4440635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑