Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,501,271	T→C	P4P (CCT→CCC)	yjgZ →	KpLE2 phage‑like element; uncharacterized protein YjgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,501,271	0	T	C	100.0%	179.9 / NA	51	P4P (CCT→CCC)	yjgZ	KpLE2 phage‑like element; uncharacterized protein YjgZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/28);  total (23/28)

CGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAG  >  NC_000913/4501136‑4501407                                                                                                                                        |                                                                                                                                         ggTTATTGTGGCGTGACAGCACCCTGGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGAc                                                                                                                                      <  1:347596/139‑1 (MQ=255)         tgGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGcttctt                                                                                                                              >  2:563269/1‑140 (MQ=255)             gtgtCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTCCAGGGAGATATTAAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTcc                                                                                                                          >  2:347596/1‑140 (MQ=255)              tgtCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCt                                                                                                                         >  1:775710/1‑140 (MQ=255)                    cACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGt                                                                                                                   <  1:595295/140‑1 (MQ=255)                    cACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGt                                                                                                                   <  1:563269/140‑1 (MQ=255)                    cACCCTCGACGGCATTTCGATGCCTGCTCAGTGTTCACTGACGTTACGCCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGt                                                                                                                   <  2:280620/140‑1 (MQ=255)                      cccTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCg                                                                                                                 <  2:775710/140‑1 (MQ=255)                       ccTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCgg                                                                                                                >  1:594274/1‑140 (MQ=255)                         tCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCACGGGCCTCTTCTTGTCCTTCCAGTCGGAg                                                                                                              >  1:280620/1‑140 (MQ=255)                              ggCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCgaga                                                                                                         >  1:84118/1‑140 (MQ=255)                                         ggCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCgggg                                                                                              <  2:594274/140‑1 (MQ=255)                                         ggCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCgggg                                                                                              <  1:665291/140‑1 (MQ=255)                                          gctgctCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGc                                                                                             <  1:729489/140‑1 (MQ=255)                                              ctCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCTAGTCGGAGCGAGAGTTAGCCGGGGCGGAc                                                                                         >  2:997570/1‑140 (MQ=255)                                                 aGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGATTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACttt                                                                                      <  1:498898/140‑1 (MQ=255)                                                   tgtTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGTCCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCa                                                                                    <  1:575127/140‑1 (MQ=255)                                                    gtTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCAc                                                                                   >  2:674761/1‑140 (MQ=255)                                                     ttCACTCACGTTACGCCCCGCACAACCCGCTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCAcc                                                                                  <  1:276993/140‑1 (MQ=255)                                                           cacGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGa                                                                            >  1:936748/1‑140 (MQ=255)                                                            acGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGaa                                                                           >  2:722196/1‑140 (MQ=255)                                                             cGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGaaa                                                                          >  1:665576/1‑140 (MQ=255)                                                             cGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGaaa                                                                          >  2:1011329/1‑140 (MQ=255)                                                               ttACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGTCGGACTTTCACCGACGGAAAGa                                                                        >  1:437535/1‑140 (MQ=255)                                                               ttACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTGCCAGTCGGCGCGAGAGATAGCCGGGCCGGACTTTCACCGACGGAAAGa                                                                        >  2:350121/1‑140 (MQ=255)                                                                  cGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAAAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCg                                                                     >  1:490109/1‑140 (MQ=255)                                                                    gCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTg                                                                   <  2:44967/140‑1 (MQ=255)                                                                    gCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGCCGGAGCGCTAGTAACCCGGGGCGGAATTTCGACGACGGAAAGATCGTg                                                                   >  2:436456/1‑140 (MQ=255)                                                                      ccGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCa                                                                 <  2:58822/140‑1 (MQ=255)                                                                        gCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCAtt                                                               <  1:848993/140‑1 (MQ=255)                                                                             aCCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAg                                                          <  2:134749/140‑1 (MQ=255)                                                                              cccGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAgg                                                         >  1:7673/1‑140 (MQ=255)                                                                               ccGTTGACTTCCTGAATAAGTCCTTTCAGGGTGAGGTTCGGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGc                                                        <  2:437535/140‑1 (MQ=255)                                                                               ccGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTGCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGc                                                        <  2:123521/140‑1 (MQ=255)                                                                                cGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGca                                                       <  2:546146/140‑1 (MQ=255)                                                                                  ttGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGcaca                                                     <  1:137984/140‑1 (MQ=255)                                                                                  ttGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGcaca                                                     <  1:1011329/140‑1 (MQ=255)                                                                                  ttGACTTCCTGAAGAAGGCGTTGCAGGGAGAGATTGAGCCCTTTTGTTGCCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGcaca                                                     <  2:379298/140‑1 (MQ=255)                                                                                   tGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAACCGGGGCGGACTATCACCGACGGAATGATCCTGCATTTCAAGGcacac                                                    >  2:290909/1‑140 (MQ=255)                                                                                         ccTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGcaca                                                     <  1:157980/133‑1 (MQ=255)                                                                                         ccTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGcaca                                                     >  2:157980/1‑133 (MQ=255)                                                                                                  gTCGTTTCAGGGAGAGTCTCAGTCCTTTTGGCACCTCCCTGACCGCTTTTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCg                                     <  2:490109/140‑1 (MQ=255)                                                                                                  gTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCg                                     <  1:691941/140‑1 (MQ=255)                                                                                                   tCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGa                                    >  1:96218/1‑140 (MQ=255)                                                                                                       ttCAGGGAGAGATTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGt                                >  2:635553/1‑140 (MQ=255)                                                                                                             gagagaTTCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTAtc                          >  1:517054/1‑140 (MQ=255)                                                                                                                    tCAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCAGTCCgg                   <  1:635553/140‑1 (MQ=255)                                                                                                                    tCAGCCCTTTTGTTAACTCCCGGACTGCTACTTGTCCCTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCgg                   <  1:350121/140‑1 (MQ=255)                                                                                                                     cAGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGt                  <  2:691961/140‑1 (MQ=255)                                                                                                                      aGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTAtccgtc                      <  2:157657/135‑1 (MQ=255)                                                                                                                      aGCCCTTTTGTTACCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTAtccgtc                      >  1:157657/1‑135 (MQ=255)                                                                                                                       gCCCTTTTGTTACCTACCGGACCGCGTCTAGTCCTTCTAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCg                <  1:369310/140‑1 (MQ=255)                                                                                                                                  aCCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCgg                   >  1:618042/1‑126 (MQ=255)                                                                                                                                  aCCTCCCGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCgg                   <  2:618042/126‑1 (MQ=255)                                                                                                                                     tcccGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAg  >  1:819676/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         CGTTATTGTGGCGTGTCAGCACCCTCGACGGCATTTCGATGGCTGCTCAGTGTTCACTCACGTTACGGCCCGCATAACCCGTTGACTTCCTGAAGAAGTCGTTTCAGGGAGAGATTCAGCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAG  >  NC_000913/4501136‑4501407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑