Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,548,979	G→A	D58N (GAT→AAT)	fimH →	type 1 fimbriae D‑mannose specific adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,548,979	0	G	A	100.0%	226.1 / NA	66	D58N (GAT→AAT)	fimH	type 1 fimbriae D‑mannose specific adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/43);  total (23/43)

CTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGC  >  NC_000913/4548841‑4549113                                                                                                                                           |                                                                                                                                       ctgATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTTTGTAAACCTTGCGCCCGTCGTGAATGTGGGGGAAAAACTGGTCGTGGa                                                                                                                                       >  2:33394/1‑140 (MQ=255)    aTGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGAGGCAACGCCAAAGTTTTTGTAAAACTTGCGCCCGTCGTGAATGTGGGGCAAAAACAGGTCGTGAATCt                                                                                                                                    >  2:583218/1‑140 (MQ=255)      gggCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAAACCTGCGCCCGTCGGGAATGTGGGGCAAAAACCGGTCGTGAATCttt                                                                                                                                  >  2:100895/1‑140 (MQ=255)      gggCTGGTCGGAAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTTAATGTGGGGCAAAACCTGGTCGTGAATCttt                                                                                                                                  <  1:227908/140‑1 (MQ=255)              cGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCAGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCaa                                                                                                                          >  1:858002/1‑140 (MQ=255)               ggTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCaaa                                                                                                                         <  1:670058/140‑1 (MQ=255)                         tGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCa                                                                                                               <  1:514964/140‑1 (MQ=255)                          ggTCTTCCGCCTGAAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCAt                                                                                                              <  2:711137/140‑1 (MQ=255)                           gTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGGACCGCCAATGTTTTTGTAAAACTTTCGCCCCGCGTGGATGTGGGGGAAAACCTGGGTGTGAAACTTTTGACGCAAATCTTTTTTCATa                                                                                                             >  2:841325/1‑140 (MQ=255)                              aTTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACg                                                                                                          <  2:794280/140‑1 (MQ=255)                                      gTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCg                                                                                                  <  1:841325/140‑1 (MQ=255)                                      gTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCg                                                                                                  <  1:758020/140‑1 (MQ=255)                                        aaaaCCGCCAATGGTTCCGCTATCCGTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGa                                                                                                <  1:366732/140‑1 (MQ=255)                                        aaaaCCGCCAATGGTCCCGCTACCCTTATTGGCGGTGTCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGa                                                                                                <  1:354850/140‑1 (MQ=255)                                        aaaaCCGCCAATGGTACCGCTATCCTTATCGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGa                                                                                                <  1:437368/140‑1 (MQ=255)                                        aaaaCCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGa                                                                                                <  2:121200/140‑1 (MQ=255)                                          agccGCCAATGGTTCCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGTaaa                                                                                              <  2:982997/138‑1 (MQ=255)                                                  aTGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCTATGTTTATGTAATCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTAT‑‑GGAAACCATTACAGa                                                                                    <  2:272546/140‑1 (MQ=255)                                                  aTGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACa                                                                                      <  1:100895/140‑1 (MQ=255)                                                  aTGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACa                                                                                      <  1:169371/140‑1 (MQ=255)                                                  aTGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACa                                                                                      <  1:96407/140‑1 (MQ=255)                                                  aTGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACa                                                                                      <  1:802841/140‑1 (MQ=255)                                                  aTGGTACCGCTATCCCTATTGGCGGAGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACa                                                                                      <  1:372152/140‑1 (MQ=255)                                                              tCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTTGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTcaca                                                                          >  1:577218/1‑140 (MQ=255)                                                                   aTTGGCGGTGGCAGCGCCCATGTTTATGTAAAACTTGCGCCCCTCGTGAATGTGGGGCAAAAACCGGGCGTGGATCTTTTGACCCAAATCTTTTTCCATAACGATTATCCGGAAAACAATTCAGACTATGTCACAATGCa                                                                     >  2:192722/1‑140 (MQ=255)                                                                          gTGGCAGCCCCCATGTTTTTGTAAAACTTGCGCCCGTCGTGAATGTGGGGCAAAAACCGGTCGTGAATCTTTTTACGCAAAACTTTTTCCATAACGATTATCCCGGAACCATTACAAACTATGTCACACTGCAACGAGGc                                                              >  2:576680/1‑140 (MQ=255)                                                                           tGGCAGCGCCAATGTTTGTGTACACCTTGCGCCCGTCGTAAATGTGGGGTAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCt                                                             <  2:298161/140‑1 (MQ=255)                                                                           tGGCAGCGCCAATGTTTATGTAAACCTTGGGCCCGTCTTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCt                                                             <  1:370812/140‑1 (MQ=255)                                                                               aGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGc                                                         <  2:231717/140‑1 (MQ=255)                                                                                       tGTTTATGTAAACCTTGCGCCCTTCGTGAATGTGGCGCACAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                 <  2:391889/140‑1 (MQ=255)                                                                                       tGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                 <  2:212781/140‑1 (MQ=255)                                                                                          ttATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGgcggcg                                              <  1:583218/140‑1 (MQ=255)                                                                                           tATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                 <  1:731045/136‑1 (MQ=255)                                                                                           tATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATggcg                                                 >  2:731045/1‑136 (MQ=255)                                                                                                 aaCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATc                                       >  2:964173/1‑140 (MQ=255)                                                                                                  aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCt                                      >  1:986860/1‑140 (MQ=255)                                                                                                  aCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCt                                      >  2:819585/1‑140 (MQ=255)                                                                                                      tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAtt                                  >  1:985076/1‑140 (MQ=255)                                                                                                      tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAtt                                  >  1:721944/1‑140 (MQ=255)                                                                                                      tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAtt                                  >  2:836145/1‑140 (MQ=255)                                                                                                      tGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAtt                                  >  2:659092/1‑140 (MQ=255)                                                                                                       gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGGAACGAGGGTCGACTGATGGCGCCGTGTTATCTAAttt                                 >  1:345230/1‑140 (MQ=255)                                                                                                       gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGGTCGTCTGATGGCGACGTGTTATCTAAttt                                 >  1:315875/1‑140 (MQ=255)                                                                                                       gcgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAAttt                                 >  2:107885/1‑140 (MQ=255)                                                                                                        cgcCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTTCAACGAGACTCGGCTTATGGCAACGTGTTATCTAAtttt                                >  2:444407/1‑140 (MQ=255)                                                                                                             gtcgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                           >  1:593607/1‑140 (MQ=255)                                                                                                             gtcgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                           <  2:721944/140‑1 (MQ=255)                                                                                                             gtcgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                           <  2:786693/140‑1 (MQ=255)                                                                                                             gtcgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGCTATCTAATTTTTCCgg                           >  1:524702/1‑140 (MQ=255)                                                                                                              tcgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                           >  1:533617/1‑139 (MQ=255)                                                                                                              tcgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                           <  2:533617/139‑1 (MQ=255)                                                                                                               cgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgggg                         <  1:248486/140‑2 (MQ=255)                                                                                                               cgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                         <  2:593607/140‑1 (MQ=255)                                                                                                               cgtGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                         <  1:192722/140‑1 (MQ=255)                                                                                                               cgtGAATGTGGGGCAAAACCTGATCTTGATTCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGa                         <  1:444407/140‑1 (MQ=255)                                                                                                                 tGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAGTTTTTCCGGGAcc                       <  1:964173/140‑1 (MQ=255)                                                                                                                 tGAATGTGGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGAcc                       <  1:33394/140‑1 (MQ=255)                                                                                                                   aaTGTGGGGCAAAACCTTGTCGCGAATCTTTTGACTCAACTCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGt                     <  2:345230/140‑1 (MQ=255)                                                                                                                       tgGGGCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTaaaa                 >  2:556916/1‑140 (MQ=255)                                                                                                                         gggCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAata               >  2:922026/1‑140 (MQ=255)                                                                                                                          ggCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                           >  1:668906/1‑127 (MQ=255)                                                                                                                          ggCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCgg                           <  2:668906/127‑1 (MQ=255)                                                                                                                          ggCAAAACCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAatat              >  2:51998/1‑140 (MQ=255)                                                                                                                               aaCCTGGTCGTGAATCTGTCGACGCAAATCTCTTGTCATAACGATTATTCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTgg         <  2:324715/140‑1 (MQ=255)                                                                                                                                aCCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGc        <  1:836145/140‑1 (MQ=255)                                                                                                                                aCCTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGc        <  2:858002/140‑1 (MQ=255)                                                                                                                                  cTGGTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAg      <  2:547178/140‑1 (MQ=255)                                                                                                                                    ggTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCACTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTa    >  2:536734/1‑140 (MQ=255)                                                                                                                                     gTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg   <  1:51998/140‑1 (MQ=255)                                                                                                                                     gTCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg   >  1:660585/1‑140 (MQ=255)                                                                                                                                     gTCGTGAATCTTTCGACACAAACCTTTTGCCATAACGATCATCCGGAATCCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg   <  2:307103/140‑1 (MQ=255)                                                                                                                                      tCGTGAATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGc  <  1:819585/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       CTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGC  >  NC_000913/4548841‑4549113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑