| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,585,505 | C→T | L419L (CTG→CTA) | hsdR ← | type I restriction enzyme EcoKI endonuclease component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,585,505 | 0 | C | T | 98.3% | 196.3 / NA | 59 | L419L (CTG→CTA) | hsdR | type I restriction enzyme EcoKI endonuclease component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (28/30); minor base G (1/0); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGG > NC_000913/4585373‑4585638 | ccccTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCtt > 1:646530/1‑140 (MQ=255) cccTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTc > 1:595220/1‑140 (MQ=255) tGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCggg < 1:178575/140‑1 (MQ=255) aTTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTg < 1:575297/140‑1 (MQ=255) ccTTTTCAACCGCGCGGTCGGCTTCTTCCTGCTAAAAACCCAGGCCCAACTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCAc < 2:279170/140‑1 (MQ=255) ccTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCAc < 2:846559/140‑1 (MQ=255) ccTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCAc > 2:837938/1‑140 (MQ=255) ccTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCACTGCCGAGCATTTCCACTAGCACTTCCGGGCGGTGCCAc > 2:430068/1‑140 (MQ=255) aaCCGCGCGGACGGCGTCTTCCTGACAATGACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTa < 1:430068/140‑1 (MQ=255) aCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCTTTTCCAGGAGCGCTGCCGGGCGGTGCCACTCGGGTaa > 1:279170/1‑140 (MQ=255) ccGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAg < 2:595220/140‑1 (MQ=255) cgcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAgg > 2:966994/1‑140 (MQ=255) gcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACt < 1:868369/130‑1 (MQ=255) gcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACt > 2:868369/1‑130 (MQ=255) gcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGc > 2:708196/1‑140 (MQ=255) gcgcgGACGGCATCTACCTGATCATCACGCAGGCCCAACTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGc < 1:269072/140‑1 (MQ=255) gcgGACGGCATCTTCCTTATAATAACGCAGGCCCACCTCTCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCtt < 2:296811/140‑1 (MQ=255) gcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCtt < 1:966994/140‑1 (MQ=255) cGGCATCTACCTGATAATAACGAAGGCCCAGTTTGATCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAc < 2:498456/140‑1 (MQ=255) gCATCTTCCTGATAAAAACGCAGGCACAGTTCGCTCAGGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACat < 1:331960/140‑1 (MQ=255) ataataACGCAGGCCTAGCTCGCTCCTGCCAGGGTTACCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTAt < 1:263693/140‑1 (MQ=255) taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg < 2:91744/140‑1 (MQ=255) taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg < 1:228482/140‑1 (MQ=255) taaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTGTCGACAAATTGCGGGTATcacg > 1:319161/1‑140 (MQ=255) cAGGCCCAACTCGCTCTTGCCATGGTTATCGTCAAACCAATGATTCTGATTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGt < 2:465091/140‑1 (MQ=255) gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg < 2:76822/132‑1 (MQ=255) gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg > 1:76822/1‑132 (MQ=255) gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTcgc > 2:766176/1‑140 (MQ=255) gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGCTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTcgc > 2:240650/1‑140 (MQ=255) cAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCtt < 2:788309/140‑1 (MQ=255) ggTTATCGGCAAACCACCGATTCTGTTTTTGTGGTTCGCAGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTg < 2:319161/140‑1 (MQ=255) tCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCtt > 2:894295/1‑140 (MQ=255) tCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCtt > 2:586765/1‑140 (MQ=255) tCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCtt > 2:902616/1‑140 (MQ=255) tCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACACATTGCGGGGATCGCGCACGTCGCGACACCAAATGCCGCTTTTGGTCtt > 2:295432/1‑140 (MQ=255) ggCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCa < 1:240650/140‑1 (MQ=255) ggCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAATGCGGGTATCACCCACATCGCGATACCAGACGCCGCTTTTGGTCTTCa > 2:418481/1‑140 (MQ=255) aaaCCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttg > 2:702809/1‑140 (MQ=255) aaaCCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttg < 2:709507/140‑1 (MQ=255) aaCCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCtt < 1:783810/126‑1 (MQ=255) aaCCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCtt > 2:783810/1‑126 (MQ=255) aaCCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttgt > 2:543830/1‑140 (MQ=255) aaCCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttgt > 2:612650/1‑140 (MQ=255) aCCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttgtt < 1:702809/140‑1 (MQ=255) cTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTgcgc < 1:766176/140‑1 (MQ=255) tCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTAt < 1:213520/140‑1 (MQ=255) gTTTTTGCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTcg > 1:842226/1‑140 (MQ=255) gCGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCg > 2:582194/1‑140 (MQ=255) cGGTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGt < 1:586765/140‑1 (MQ=255) gTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGGTGCGAGGTATTCGCGCCCGTTg > 2:407115/1‑140 (MQ=255) gTTCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATAACAGATGCCGCTTATGGGCTTCATTGTTGCGCGGTATTCGCGCCCGTTg > 2:431000/1‑140 (MQ=255) tCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGt < 2:646530/140‑1 (MQ=255) tCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGt > 2:17213/1‑140 (MQ=255) tCGCTGCCGAGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGt < 1:113906/140‑1 (MQ=255) aGCATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCa > 2:886236/1‑140 (MQ=255) cATTTCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGa > 2:535818/1‑140 (MQ=255) ttCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAgg < 2:1002075/140‑1 (MQ=255) ttCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAgg < 1:582194/140‑1 (MQ=255) ttCCAGTAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGATCTTCATTGTTGGGCGTTATTCGCGACCGTAGGTCGAGTAGCAGAAgg > 2:291384/1‑140 (MQ=255) | CCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGG > NC_000913/4585373‑4585638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |