Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,092	G→A	A260T (GCG→ACG)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,092	0	G	A	100.0%	203.3 / NA	62	A260T (GCG→ACG)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/34);  total (28/34)

ACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAA  >  NC_000913/4627955‑4628226                                                                                                                                          |                                                                                                                                       aCCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACg                                                                                                                                      >  2:646769/1‑140 (MQ=255)           cTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAatat                                                                                                                             <  1:913004/139‑1 (MQ=255)           cTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAatat                                                                                                                             >  2:913004/1‑139 (MQ=255)             aCTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGc                                                                                                                          <  1:611022/140‑1 (MQ=255)               tGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTGAACTCCGCCAGTAAGTGGGAATCTgtctc                                                                                                                        >  1:316182/1‑136 (MQ=255)                 ggAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCcgcg                                                                                                                      >  1:29189/1‑140 (MQ=255)                 ggAACATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAACATCTTCAACACTACTAGTACGAGGGAATATGGCcgcg                                                                                                                      >  1:338723/1‑140 (MQ=255)                       tatTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATg                                                                                                                <  1:829312/140‑1 (MQ=255)                        atTCCTACCGAAGTGAAGCCGTTTTTTGTCCGTACCGGGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGt                                                                                                               <  1:367335/140‑1 (MQ=255)                             tACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCtttt                                                                                                          >  2:781883/1‑140 (MQ=255)                             tACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCtttt                                                                                                          >  2:675497/1‑140 (MQ=255)                             tACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCtttt                                                                                                          >  1:191812/1‑140 (MQ=255)                             tACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCtttt                                                                                                          >  1:987047/1‑140 (MQ=255)                              aCCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATg                                                                                                                <  1:29847/133‑1 (MQ=255)                              aCCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATg                                                                                                                >  2:29847/1‑133 (MQ=255)                               ccGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTca                                                                                                        >  2:68086/1‑140 (MQ=255)                               ccGAAGTGAAGCCGTATTTTGTCCGTACCTTGGCGATTCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTca                                                                                                        <  1:313323/140‑1 (MQ=255)                                cGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTcac                                                                                                       >  1:137914/1‑140 (MQ=255)                                cGAAGTGAAGCCGCTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTcac                                                                                                       >  2:174271/1‑140 (MQ=255)                                    gTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCt                                                                                                   <  2:987047/140‑1 (MQ=255)                                      gAAGCCGTTTTTTGTCCGTACCTTGCCGACCCTTGGCCGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTcg                                                                                                 <  1:307351/140‑1 (MQ=255)                                         gCCGTTTTTTGTGCGTAGCGTGTCGATCCTTAGCGTCGATTCGAGCGGGAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTcggcg                                                                                              <  2:373266/140‑1 (MQ=255)                                         gCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTcggcg                                                                                              <  1:142898/140‑1 (MQ=255)                                             ttttttGTGCGTACCTTGGCGAGCCCTGGCGACGAGTTGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGtga                                                                                          <  1:264410/140‑1 (MQ=255)                                             ttttttGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGtga                                                                                          >  1:789796/1‑140 (MQ=255)                                                tttGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGtgatga                                                                                       <  1:68086/140‑1 (MQ=255)                                                   gtgCGTACCGTGGCGATCCTTGTCGGCGAGTTGAGGGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGAt                                                                                    <  2:394165/140‑1 (MQ=255)                                                     gCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCg                                                                                  <  2:191812/140‑1 (MQ=255)                                                     gCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCg                                                                                  <  1:501314/140‑1 (MQ=255)                                                       gTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCa                                                                                <  1:691864/140‑1 (MQ=255)                                                       gTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCa                                                                                <  2:38630/140‑1 (MQ=255)                                                          ccGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTg                                                                             <  2:137914/140‑1 (MQ=255)                                                            gTGGCGATCCTTGGCGGGGAGTTGAGCGGTAAATCTACCTTGGTAAACCAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCa                                                                           <  2:338723/140‑1 (MQ=255)                                                            gTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCATCACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCa                                                                           <  1:738253/140‑1 (MQ=255)                                                            gTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCa                                                                           <  2:889638/140‑1 (MQ=255)                                                            gTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCa                                                                           <  2:29189/140‑1 (MQ=255)                                                                  aTCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTATTCCCACATCGGCGGTAATGAGATCGCATTGCAGTATTc                                                                     >  2:395606/1‑140 (MQ=255)                                                                      ttGGCGGCGAGTCGATCGGTAAGTCCACCCTGGTAAAAAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGAc                                                                 <  1:351944/140‑1 (MQ=255)                                                                      ttGGCGGCGAGTCGAGCGGTAACTCCACCTTGGTAAAAAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGAc                                                                 <  1:395606/140‑1 (MQ=255)                                                                      ttGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGAc                                                                 <  1:159100/140‑1 (MQ=255)                                                                      ttGGCGGCGAGTCGAACGGTAATTCCACCCTGGTAAAGAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGAc                                                                 <  1:371828/140‑1 (MQ=255)                                                                        ggcggcGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTa                                                               >  2:66995/1‑140 (MQ=255)                                                                         gcggcgAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCCCGGCGGTCATGCGACCGCATTCCAGTATTCTGACTAc                                                              >  1:436538/1‑140 (MQ=255)                                                                                        gTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGACCGCATTGGAGTATTCTGACTATGATAAAATCGCGCTg                                               >  1:436148/1‑140 (MQ=255)                                                                                           aaTCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGc                                            <  1:965326/140‑1 (MQ=255)                                                                                            aTCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGcc                                           <  1:781883/140‑1 (MQ=255)                                                                                            aTCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGcc                                           <  1:66995/140‑1 (MQ=255)                                                                                            aTCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACCTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGcc                                           <  2:351058/140‑1 (MQ=255)                                                                                             tCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCa                                          <  2:783668/140‑1 (MQ=255)                                                                                              ccACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCAc                                         >  1:898544/1‑140 (MQ=255)                                                                                                 cccTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCt                                      >  2:561569/1‑140 (MQ=255)                                                                                                                    cAATATCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCgg                   <  1:784735/140‑1 (MQ=255)                                                                                                                      atatCTTCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTg                 >  1:804427/1‑140 (MQ=255)                                                                                                                            tCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAatat           <  2:804427/140‑1 (MQ=255)                                                                                                                            tCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAatat           <  1:623227/140‑1 (MQ=255)                                                                                                                            tCAACACCACCAGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGAGAAAATCGCACTGCGCCACGCTCAATTCATTTATTTTGCGGTGAAatat           >  2:346440/1‑140 (MQ=255)                                                                                                                                   caccaGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATa    >  1:893405/1‑140 (MQ=255)                                                                                                                                   caccaGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATa    >  2:979133/1‑140 (MQ=255)                                                                                                                                   caccaGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATa    >  1:23952/1‑140 (MQ=255)                                                                                                                                   caccaGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATa    >  2:855576/1‑140 (MQ=255)                                                                                                                                    accaGTACGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaa   >  2:184757/1‑140 (MQ=255)                                                                                                                                     ccaGTACGTGCGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaaa  >  1:66127/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAA  >  NC_000913/4627955‑4628226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑