Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,159	C→T	A122T (GCC→ACC)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,159	0	C	T	100.0%	122.4 / NA	38	A122T (GCC→ACC)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/18);  total (20/18)

TTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGC  >  NC_000913/4630022‑4630286                                                                                                                                          |                                                                                                                                ttCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCTTGAATGATCTCGTCACGACGGGCTTGTTCAGCGGccc                                                                                                                               >  1:278156/1‑139 (MQ=255)           gagaGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTGCAGACGGCCTTGTTCAGCGGTCAGCTTGTCaaa                                                                                                                     >  2:442755/1‑140 (MQ=255)                   aGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCAt                                                                                                             >  1:172511/1‑140 (MQ=255)                   aGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTCCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCAt                                                                                                             <  1:1606/140‑1 (MQ=255)                   aGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTCCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCAt                                                                                                             >  2:1606/1‑140 (MQ=255)                          ttCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTACAGACGGCGTTGGTCAACGGTCAGCCTGTCGAAATCGGCATCCGGATc                                                                                                      >  2:277831/1‑140 (MQ=255)                               gTCCCAGTCCGGCAGACGTAGCGCATCCGCCACACGCTCTAGCTGTACCTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGt                                                                                                 <  2:415935/140‑1 (MQ=255)                                 cccAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTAc                                                                                               <  1:442755/140‑1 (MQ=255)                                  ccAGTCCGGCGGACGTGGCGCATCCGCCGCGCGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTGCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGTCATCCGGATCGGCGTACa                                                                                              <  2:808791/140‑1 (MQ=255)                                           cAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATaca                                                                                     <  2:806584/140‑1 (MQ=255)                                                 tAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCAt                                                                               <  2:102955/140‑1 (MQ=255)                                                 tAGCGCATCCGCCGCACACTCCAGCTGTACGTTCAAATTGTGACCGTCTTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCAt                                                                               <  2:410308/140‑1 (MQ=255)                                                      cATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAgg                                                                          <  2:137470/140‑1 (MQ=255)                                                       aTCCGCCGCACGCTCGAGCTGTTCGATCATATTATGATCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGc                                                                         <  1:432134/140‑1 (MQ=255)                                                        tCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCg                                                                        >  2:799564/1‑140 (MQ=255)                                                        tCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCg                                                                        >  1:238010/1‑140 (MQ=255)                                                             cgcACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCa                                                                   <  1:685077/140‑1 (MQ=255)                                                             cgcACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCa                                                                   <  2:172511/140‑1 (MQ=255)                                                                         cTGTACGTTCAGATTCTGACCGCCGTGAGTCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCa                                                       <  1:277831/140‑1 (MQ=255)                                                                              cGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCt                                                  >  1:921706/1‑140 (MQ=255)                                                                              cGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCt                                                  >  1:95153/1‑140 (MQ=255)                                                                              cGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCt                                                  >  2:652875/1‑140 (MQ=255)                                                                                ttCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGa                                                >  2:610231/1‑140 (MQ=255)                                                                                      ttATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGc                                          <  1:799564/140‑1 (MQ=255)                                                                                         tGACCGTCGTGAGCCTGCATGACCTCTTCCATACGTCCTTGTTCAGCGCTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGcttc                                       <  2:269472/140‑1 (MQ=255)                                                                                                cgtGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATg                                <  2:921706/140‑1 (MQ=255)                                                                                                    agtcTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCCCTCCTGAAACCGCTTCTTCAATGGACt                            <  2:901328/137‑1 (MQ=255)                                                                                                         gAATGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTcacgc                       <  2:238010/140‑1 (MQ=255)                                                                                                          aattaTCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTGACCACTTCTGACACGGCTTCTTCAATGGACTcacgca                      >  1:354289/5‑140 (MQ=255)                                                                                                          aaTGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTcacgca                      >  2:746276/1‑140 (MQ=255)                                                                                                          aaTGATCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTcacgca                      >  1:506953/1‑140 (MQ=255)                                                                                                              aTCTCTTCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGgt                  >  1:654393/1‑140 (MQ=255)                                                                                                                   ttCCAGACGGCCTTGTTTAGCGTTCAGCTTGTCAAAATCGGCATCCGGGTCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGtt             <  2:336800/140‑1 (MQ=255)                                                                                                                   ttCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGtt             <  2:986397/140‑1 (MQ=255)                                                                                                                    tCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTc            >  2:18008/1‑140 (MQ=255)                                                                                                                    tCCAGACGGCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTAAACCACTTCTGAAACCGCTTCTTCACTGGAATCACGCACGGTGTGTTc            >  2:357721/1‑140 (MQ=255)                                                                                                                           ggCCTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTc     >  2:40203/1‑140 (MQ=255)                                                                                                                             ccTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAg   >  2:940851/1‑140 (MQ=255)                                                                                                                             ccTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTCCTTCAATTGACTCAGGCACGGTGTGTTCCGGGTTCAg   >  1:336800/1‑140 (MQ=255)                                                                                                                             ccTTGTTCAGCGGTCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCG‑TTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGc  >  2:890098/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                TTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGC  >  NC_000913/4630022‑4630286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑