Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,175	A→G	intergenic (+55/‑3)	creC → / → creD	sensory histidine kinase CreC/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,175	0	A	G	100.0%	165.5 / NA	47	intergenic (+55/‑3)	creC/creD	sensory histidine kinase CreC/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/18);  total (29/18)

AACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGA  >  NC_000913/4638043‑4638310                                                                                                                                     |                                                                                                                                        aaCGGCGAAGTCACGCAGCGCAACGTGCAGGCAAGCGGCATGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGtt                                                                                                                                  <  2:437980/140‑1 (MQ=255)      cGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACAGAGTCTTCGAATCGTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAAt                                                                                                                             >  2:310318/1‑140 (MQ=255)        aaGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATATTCTTCGTAGCCTGCTGGCATTGCAAAGGAGGAGGCTATGTTGAAATcc                                                                                                                           >  2:390179/1‑140 (MQ=255)           tCACGCTGCGCAACGTGCAGGACGGTGGCGTCCTGTCCTTGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATccccc                                                                                                                        <  2:298702/140‑1 (MQ=255)           tCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATccccc                                                                                                                        <  2:979659/140‑1 (MQ=255)           tCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATccccc                                                                                                                        <  2:12869/140‑1 (MQ=255)                      aaCGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGaa                                                                                                             <  1:960688/140‑1 (MQ=255)                      aaCGTGCAGGAAGGTCGCGTGCCGGCCTCCCTTCGACCTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGaa                                                                                                             <  1:310318/140‑1 (MQ=255)                        cGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGaaaa                                                                                                           <  1:890870/140‑1 (MQ=255)                             aGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACt                                                                                                      <  2:712888/140‑1 (MQ=255)                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGt                                                                                               >  2:637965/1‑140 (MQ=255)                                    tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGt                                                                                               >  2:969575/1‑140 (MQ=255)                                     ggCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                                                                              <  1:637965/140‑1 (MQ=255)                                        gTGCTGGCCTCGCTTCGACTTCCCCCTCAGTTCACATTGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTgg                                                                                           <  1:359434/140‑1 (MQ=255)                                        gTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTCGCCTGTTTgg                                                                                           <  2:911982/140‑1 (MQ=255)                                           cTGGCCTCGCTTCGATTTCACCTTCTCTTAACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGc                                                                                        <  1:390179/140‑1 (MQ=255)                                           cTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGc                                                                                        >  2:660911/1‑140 (MQ=255)                                                       cGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCAGGAAAATGACTAGCGTGTTTGTTGCAGTATTGCtgtt                                                                            >  1:496400/1‑140 (MQ=255)                                                             cACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGAt                                                                      >  1:214335/1‑140 (MQ=255)                                                                      ttCACATAGCTTCAATTTCTTCTCACATAATCTTCGTTTCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAAt                                                             <  1:337027/140‑1 (MQ=255)                                                                        cacaTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTATGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGc                                                           >  2:784624/1‑140 (MQ=255)                                                                          caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGG‑AAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGa                                                        >  1:166937/1‑140 (MQ=255)                                                                            tAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGAt                                                       >  2:167774/1‑140 (MQ=255)                                                                             aGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGTTGCAGTAGTGCTGTAGTTGATTCCGATAATGCTGAtt                                                      >  1:488693/1‑140 (MQ=255)                                                                              gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTc                                                     >  2:924785/1‑140 (MQ=255)                                                                              gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTc                                                     >  2:124468/1‑140 (MQ=255)                                                                               cTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTACTGGTGCTGATTCCGATAATGCTGATTCg                                                    >  1:385874/1‑140 (MQ=255)                                                                                ttCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGTAGTATTGGTGTGGTCGATTCCAATAATGCTGATTCgg                                                   >  1:322804/1‑140 (MQ=255)                                                                                ttCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGAGCAGTATTGCTGTTGTGGATTACGATAATGCTGATTCgg                                                   >  2:337027/1‑140 (MQ=255)                                                                                  cAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCa                                                 >  1:807824/1‑140 (MQ=255)                                                                                    aaTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAgg                                               <  1:876638/140‑1 (MQ=255)                                                                                         ttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGAtt                                          >  2:636768/1‑140 (MQ=255)                                                                                                   gTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAAGGCTTATTCGGCAGTTGATTGTCGAACGTg                                >  1:349279/1‑140 (MQ=255)                                                                                                   gTCTTCGTATCCTGCAGCCATTTCATAGGCGAAGGCTTTGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTg                                <  1:452839/140‑1 (MQ=255)                                                                                                      ttCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTg                             >  1:710008/1‑140 (MQ=255)                                                                                                      ttCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTg                             >  1:726588/1‑140 (MQ=255)                                                                                                        cGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGAt                           <  1:124468/140‑1 (MQ=255)                                                                                                        cGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGAt                           <  1:924785/140‑1 (MQ=255)                                                                                                          tATCCTGCTGCCATTGCAAAGGTGAAGGCTACGATAAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACATGCTGATTa                         <  2:496400/140‑1 (MQ=255)                                                                                                          tATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAGTGCTGATGCGGAAGGTGATTGTCGAACGTGCTGATTa                         >  1:264157/1‑140 (MQ=255)                                                                                                              ctgctgCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGt                     >  2:720750/1‑140 (MQ=255)                                                                                                              ctgctgCCATTGCAAAGGACAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGAGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGt                     >  1:630387/1‑140 (MQ=255)                                                                                                               tgctgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTa                    >  2:756192/1‑140 (MQ=255)                                                                                                                gctgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAg                   <  1:167462/140‑1 (MQ=255)                                                                                                                gctgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAg                   >  2:85157/1‑140 (MQ=255)                                                                                                                gctgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTATGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAg                   <  1:784624/140‑1 (MQ=255)                                                                                                                  tgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCg                 >  1:729244/1‑140 (MQ=255)                                                                                                                      aTTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGAtgt             >  1:106117/1‑140 (MQ=255)                                                                                                                              ggAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTGGCGATGTGGAAGATg     >  1:371111/1‑140 (MQ=255)                                                                                                                                 gaaggCTATGTTGAAAACCCCCTTGTTCTGGAATAGGACAAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGa  <  2:452109/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        AACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGA  >  NC_000913/4638043‑4638310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑