Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	556,489	A→G	V22A (GTC→GCC)	ybcI ←	conserved inner membrane protein YbcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	556,489	0	A	G	100.0%	154.9 / NA	44	V22A (GTC→GCC)	ybcI	conserved inner membrane protein YbcI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/27);  total (17/27)

CGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAA  >  NC_000913/556353‑556625                                                                                                                                         |                                                                                                                                         cGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCtt                                                                                                                                       <  1:874623/140‑1 (MQ=255) cGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCtt                                                                                                                                       <  1:8150/140‑1 (MQ=255)  gCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCttt                                                                                                                                      <  1:902461/140‑1 (MQ=255)        acCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGaacc                                                                                                                                <  1:710162/140‑1 (MQ=255)         ccAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGaaccc                                                                                                                               <  2:22641/140‑1 (MQ=255)         ccAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGaaccc                                                                                                                               <  1:920179/140‑1 (MQ=255)         ccAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGaaccc                                                                                                                               <  1:673821/140‑1 (MQ=255)            gCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACGGACGCGGAGGGATAGCTTTCGaacccaac                                                                                                                            >  1:356706/1‑140 (MQ=255)              gAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGaacccaaccc                                                                                                                          <  1:598781/140‑1 (MQ=255)                aTGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTa                                                                                                                        <  2:766547/140‑1 (MQ=255)                aTGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTa                                                                                                                        <  2:653322/140‑1 (MQ=255)                  gggTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTaaa                                                                                                                      >  2:903807/1‑140 (MQ=255)                  gggTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTaaa                                                                                                                      >  1:585547/1‑140 (MQ=255)                     tAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCa                                                                                                                   <  1:903807/140‑1 (MQ=255)                       aaCCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAAt                                                                                                                 <  2:25173/140‑1 (MQ=255)                             cgATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATg                                                                                                                >  2:244447/1‑135 (MQ=255)                             cgATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATg                                                                                                                <  1:244447/135‑1 (MQ=255)                             cgATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAg                                                                                                           >  1:756090/1‑140 (MQ=255)                             cgATGACCAAAAACAATGCCGTCAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAg                                                                                                           <  1:356822/140‑1 (MQ=255)                                         aCATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACggcggc                                                                                               <  1:940651/140‑1 (MQ=255)                                            ttGCCGTAAGCAACGTCAAATTCAAGCGAAAATACGTTGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtg                                                                                            <  1:480079/140‑1 (MQ=255)                                            ttGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtg                                                                                            >  1:841995/1‑140 (MQ=255)                                             tGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgc                                                                                           >  1:180873/1‑140 (MQ=255)                                              gCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACggcggc                                                                                               >  2:677734/1‑135 (MQ=255)                                              gCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACggcggc                                                                                               <  1:677734/135‑1 (MQ=255)                                              gCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGgcgtgcg                                                                                          >  1:754409/1‑140 (MQ=255)                                                    aGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGtaataa                                                                                    <  1:691517/140‑1 (MQ=255)                                                         cGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGtt                                                                               >  1:848880/1‑140 (MQ=255)                                                         cGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGtt                                                                               >  2:718399/1‑140 (MQ=255)                                                                        aCAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCt                                                                <  2:677273/140‑1 (MQ=255)                                                                                   gcgtcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATg                                                     <  2:841995/140‑1 (MQ=255)                                                                                    cgtcggGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGt                                                    >  1:122073/1‑140 (MQ=255)                                                                                         ggCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATg                                               <  1:797630/140‑1 (MQ=255)                                                                                         ggCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATg                                               <  1:736319/140‑1 (MQ=255)                                                                                          gcagcaTCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGc                                              <  2:180873/140‑1 (MQ=255)                                                                                           cagcaTCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCt                                             <  2:848880/140‑1 (MQ=255)                                                                                                           atTCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAAc                             >  1:373591/1‑140 (MQ=255)                                                                                                             tCCGGCAAATAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACgg                           >  1:887755/1‑140 (MQ=255)                                                                                                                   aaaTAACAGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCAAGATGGTCGATTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTa                     <  2:199059/140‑1 (MQ=255)                                                                                                                          aGACGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCa              <  2:756090/140‑1 (MQ=255)                                                                                                                            aCGCGGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCaaa            >  1:81621/1‑140 (MQ=255)                                                                                                                               cgGAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGt         <  2:81621/140‑1 (MQ=255)                                                                                                                                 gAGGGATGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCt       >  1:138188/1‑140 (MQ=255)                                                                                                                                      aTGGCTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGaaaa  >  2:520254/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCAAACACCAGCGAATGGGTAAACCCGCGATGACCAAAAACATTGCCGTAAGCAACGCCAAATTTAAACGACAATACGTCGGCGTCGGGCAGCATCGCCAGGATGATTCCGGCAAATAACAGACGCGGAGGGATGACTTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAA  >  NC_000913/556353‑556625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑