Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,506	C→T	A515T (GCC→ACC)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,506	0	C	T	100.0%	133.9 / NA	40	A515T (GCC→ACC)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/18);  total (22/18)

CCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCT  >  NC_000913/68374‑68640                                                                                                                                     |                                                                                                                                       ccTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCAttttt                                                                                                                                 >  2:674734/1‑140 (MQ=255)   tgtgCCGGGGCGGAACTTGGAACATAGTGTTGTTCGGTGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTg                                                                                                                               <  1:380382/140‑1 (MQ=255)            gCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTgctgagctga                                                                                                                      <  1:734185/140‑1 (MQ=255)               gAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATgg                                                                                                                   >  1:773727/1‑140 (MQ=255)                 aGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGa                                                                                                                 <  1:674734/140‑1 (MQ=255)                                   ttCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTcg                                                                                               >  2:724790/1‑140 (MQ=255)                                   ttCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCAGAGGGGATGACTGCGTGCACTTTcg                                                                                               >  1:432606/1‑140 (MQ=255)                                    tCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGGGCTAATGGGATGTGTGCGTGCACTTTcgc                                                                                              >  1:287412/1‑140 (MQ=255)                                    tCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTcgc                                                                                              >  1:204002/1‑140 (MQ=255)                                    tCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTACAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGAACTGGTCAATTTATGCAAAGCTGATGGGATAACTGCGTGGACTTTcgc                                                                                              >  2:475913/1‑140 (MQ=255)                                        cgcTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCgcggcg                                                                                          >  2:960991/1‑140 (MQ=255)                                        cgcTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCgcggcg                                                                                          >  2:521182/1‑140 (MQ=255)                                             aTCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGgc                                                                                     >  2:859541/1‑140 (MQ=255)                                                               cggcgATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCAcc                                                                   >  2:699417/1‑140 (MQ=255)                                                                 gcgATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGa                                                                 <  1:521182/140‑1 (MQ=255)                                                                  cgATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAg                                                                >  1:746121/1‑140 (MQ=255)                                                                      aaaGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGca                                                            <  2:773727/140‑1 (MQ=255)                                                                      aaaGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGca                                                            <  1:306/140‑1 (MQ=255)                                                                              tCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGTCGGCAGCTAAATTCTCCGCACTGAGCGCACAGCACTg                                                    >  1:324390/1‑140 (MQ=255)                                                                              tCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTg                                                    >  1:654151/1‑140 (MQ=255)                                                                                       tgtgCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAGAAATCGCGGCAGCGGGCGCACGGCACTGGTCAGAGGc                                           >  1:295069/1‑140 (MQ=255)                                                                                       tgtgCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGc                                           >  2:44242/1‑140 (MQ=255)                                                                                       tgtgCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGc                                           >  2:686661/1‑140 (MQ=255)                                                                                        gtgCCTGCTCGCTGCTCGGTTGTAGAGTTATCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGca                                          <  1:475913/140‑1 (MQ=255)                                                                                                  gCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCa                                <  1:724790/140‑1 (MQ=255)                                                                                                     gCACGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCg                             <  1:686661/140‑1 (MQ=255)                                                                                                       aCGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCggg                           <  2:46449/140‑1 (MQ=255)                                                                                                       aCGGTTGCAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCACCGCACCGAGCGCACAGCACTGATCAGAGGCAACAATTTGCAGCggg                           <  2:641602/140‑1 (MQ=255)                                                                                                              cAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTGAGAGGCGACAATTAGCAGCGGGCGATTCa                    >  1:287443/1‑140 (MQ=255)                                                                                                              cAGGGTTTTCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCa                    >  1:690192/1‑140 (MQ=255)                                                                                                               aGGGTTTTCTCTACCGCCCTGGTCATTTTTTGCTGGGCTGATGGGATGCCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAg                   <  2:432606/140‑1 (MQ=255)                                                                                                                    tttCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGt              <  2:654151/140‑1 (MQ=255)                                                                                                                     ttCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCACAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTc             >  2:649118/1‑140 (MQ=255)                                                                                                                     ttCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCggg                           >  1:243247/1‑126 (MQ=255)                                                                                                                     ttCTCTACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCggg                           <  2:243247/126‑1 (MQ=255)                                                                                                                         ctACCGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCgcag         <  2:776519/140‑1 (MQ=255)                                                                                                                             cGCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAgg     >  1:983136/1‑140 (MQ=255)                                                                                                                             cGCACTGGTCATTTTTTGCTGACCTGATGGGCTGTTTGCCTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAACACGTCGCAGCAgg     <  2:295069/140‑1 (MQ=255)                                                                                                                              gCACTGGTCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGc    <  1:859541/140‑1 (MQ=255)                                                                                                                              gCACTGGTCATTTTTTGCTGAGCGGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCGCCGAGCGCACAGCACTGGTCAGAGACAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGc    <  1:44242/140‑1 (MQ=255)                                                                                                                                aCTGGTCATTTTTTGCTGAGCTTATTGGACGTCTGCGCGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCt  <  1:257405/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGGATGTCTGCGTGCACTTTCGCGGCGACGGCAGCAAAAATCGCCGCACCGAGCGCACAGCACTGGTCAGAGGCAACAATTTGCAGCGGGCGATTCAGCACGTCGCAGCAGGCCT  >  NC_000913/68374‑68640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑