Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	647,203	A→G	F103L (TTC→CTC)	citG ←	triphosphoribosyl‑dephospho‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	647,203	0	A	G	100.0%	105.5 / NA	33	F103L (TTC→CTC)	citG	triphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/15);  total (18/15)

AAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGA  >  NC_000913/647067‑647337                                                                                                                                         |                                                                                                                                       aaaCTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGGCTTTATGAGTGATTACGCCCGCAGTGGCGCGGAGCat                                                                                                                                     >  1:338797/1‑140 (MQ=255) aaaCTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCat                                                                                                                                     >  1:117734/1‑140 (MQ=255)      tGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCAc                                                                                                                                <  2:87704/140‑1 (MQ=255)      tGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATACGTGTTTACGCCAGCAGGGGCGCGGAGCATATCAc                                                                                                                                >  2:377160/1‑140 (MQ=255)           ccgTAGAATAAACGGTCGTTGGTGTTACCGGTTTGTTGATTTGAAGCAACCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGAAGTGGCGCGGAGCATATCACCTTCg                                                                                                                           <  2:487527/140‑1 (MQ=255)                gAACAAACGGTTGTTTGCGTTACCGTTTGGTTGAGTTTAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGc                                                                                                                      <  1:413787/140‑1 (MQ=255)                 aaCAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGcc                                                                                                                     >  1:855414/1‑140 (MQ=255)                        ggTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCaa                                                                                                              >  2:701493/1‑140 (MQ=255)                         gttgttGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAAt                                                                                                             <  1:936562/140‑1 (MQ=255)                               ggCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCg                                                                                                       >  2:628540/1‑140 (MQ=255)                                    tACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGTATATCACTTTCTCAGGCCATAACAATTGGGCGTAATc                                                                                                  >  1:319377/1‑140 (MQ=255)                                    tACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATc                                                                                                  >  2:577145/1‑140 (MQ=255)                                         gttggttgAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTgg                                                                                             <  1:577145/140‑1 (MQ=255)                                           tggttgAGTTGAAGCAAACGGCTAATTGCCGCACACAGCTGCCCTAAAAAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAg                                                                                           <  2:338797/140‑1 (MQ=255)                                            ggttgAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTGCCCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGt                                                                                          <  2:848865/140‑1 (MQ=255)                                                     gaAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCt                                                                                 <  1:92344/140‑1 (MQ=255)                                                          aaaCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGc                                                                            <  2:855414/140‑1 (MQ=255)                                                             cGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAt                                                                         <  2:1011194/140‑1 (MQ=255)                                                              ggCCAATTGCCGCACGTAGCAGCCCCAAACAAAAAATCCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAtt                                                                        <  1:344687/140‑1 (MQ=255)                                                                ccAATTGCCGCACATAGCGGCCCAAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTc                                                                      <  1:111878/140‑1 (MQ=255)                                                                ccAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTc                                                                      >  1:196412/1‑140 (MQ=255)                                                                          cacaTAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAg                                                            <  1:628540/140‑1 (MQ=255)                                                                                    cccTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAAtt                                                  >  2:237349/1‑140 (MQ=255)                                                                                         aaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATg                                             <  1:691495/140‑1 (MQ=255)                                                                                              aaaaTGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACg                                        >  2:820352/1‑140 (MQ=255)                                                                                                              gCGTGTTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGa                        >  2:1012632/1‑140 (MQ=255)                                                                                                                 tgtTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATc                     >  2:256595/1‑140 (MQ=255)                                                                                                                 tgtTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATc                     >  1:769426/1‑140 (MQ=255)                                                                                                                 tgtTTACGCCCGCAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATc                     >  1:227266/1‑140 (MQ=255)                                                                                                                          ccGAAGTGGCGCGGAGTATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAgcgcg            <  2:227266/140‑1 (MQ=255)                                                                                                                             cAGTGGCGCGGAGCATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAAt                      <  2:815938/127‑1 (MQ=255)                                                                                                                             cAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCGCCAAATTCAATGTAGCCGGGTAGCCTGCCCCGAATCGCCAGCGCGCTg         >  2:440726/1‑140 (MQ=255)                                                                                                                             cAGTGGCGCGGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAAt                      >  1:815938/1‑127 (MQ=255)                                                                                                                                gggCGCGGAGCATTTCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCgg      <  2:196412/139‑1 (MQ=255)                                                                                                                                    gcgGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGa  <  1:1012632/140‑1 (MQ=255)                                                                                                                                    gcgGAGCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGGACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGa  >  2:612568/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGA  >  NC_000913/647067‑647337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑