Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,244	A→G	V325A (GTT→GCT)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,244	0	A	G	100.0%	143.0 / NA	40	V325A (GTT→GCT)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/15);  total (25/15)

CCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCAC  >  NC_000913/667108‑667376                                                                                                                                         |                                                                                                                                     ccAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGccc                                                                                                                                   <  2:910417/140‑1 (MQ=255)            aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGgcg                                                                                                                        <  2:699405/140‑1 (MQ=255)                         aCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCg                                                                                                           <  2:612463/140‑1 (MQ=255)                         aCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCg                                                                                                           <  2:21936/140‑1 (MQ=255)                           cTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGgt                                                                                                         <  1:733702/140‑1 (MQ=255)                                     ccaccaGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGAt                                                                                               >  1:919556/1‑140 (MQ=255)                                                 gTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAgg                                                                                   <  1:738012/140‑1 (MQ=255)                                                     aaCAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGa                                                                               >  1:46232/1‑140 (MQ=255)                                                          cgtcgtATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaataat                                                                          >  2:195406/1‑140 (MQ=255)                                                            tcgtATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCt                                                                        >  2:835011/1‑140 (MQ=255)                                                            tcgtATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCt                                                                        <  1:869324/140‑1 (MQ=255)                                                            tcgtATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCt                                                                        <  1:857482/140‑1 (MQ=255)                                                               tATGGCGCGTGATCCCCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg                                                                     <  2:1016678/140‑1 (MQ=255)                                                                 ttGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCt                                                                   <  2:768014/140‑1 (MQ=255)                                                                      cgTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGgaga                                                              >  1:37337/1‑140 (MQ=255)                                                                              ccccGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTCTTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTca                                                      >  2:376511/1‑140 (MQ=255)                                                                                  ggCGCTCAATGCCGAAACCGCCTCACAGGGTTTAACTCTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTcaacaa                                                  <  1:441899/140‑1 (MQ=255)                                                                                         aaTGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGtt                                           <  2:627274/140‑1 (MQ=255)                                                                                         aaTGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGtt                                           <  2:567303/140‑1 (MQ=255)                                                                                              cGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACCAGGCGGAATAATCTCTCCTGGAGATACCGTCAACAAACAAGTTTgggc                                      >  2:441899/1‑139 (MQ=255)                                                                                              cGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGt                                      >  2:662674/1‑140 (MQ=255)                                                                                                                tttAACTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGaaa                    >  1:760202/1‑140 (MQ=255)                                                                                                                   aaCTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAAcc                  <  2:905955/139‑1 (MQ=255)                                                                                                                   aaCTGTAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAAcc                  >  1:905955/1‑139 (MQ=255)                                                                                                                        tAGACGCTGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGcc            >  2:85243/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACGAACGAGTATGGGTCATGACTAAGCGTGGAAACCAGCGcc            >  2:281376/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAGACCAGCGcc            >  1:793548/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGcc            >  1:157535/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGcc            >  2:241974/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGcc            >  1:137036/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGcc            >  1:743561/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGcc            >  1:780721/1‑140 (MQ=255)                                                                                                                        tAGACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGcc            >  1:852670/1‑140 (MQ=255)                                                                                                                          gACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAg          >  1:501980/1‑140 (MQ=255)                                                                                                                          gACGCGGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAg          >  1:53844/1‑140 (MQ=255)                                                                                                                              cgGGAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCAcc      >  2:606941/1‑140 (MQ=255)                                                                                                                               gggAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCAccc     <  2:37337/140‑1 (MQ=255)                                                                                                                               gggAGGATAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGACGTAACTAGGGGTGGAAACCAGCGCCAGCAccc     >  2:474568/1‑140 (MQ=255)                                                                                                                                  aggaTAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCAc  >  2:119096/1‑140 (MQ=255)                                                                                                                                  aggaTAAGCCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCAc  >  1:964180/1‑140 (MQ=255)                                                                                                                                  aggaTAAGCCCCCTGAGTGGCGTGGATCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCAc  <  1:474568/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTCAACAAACAAGTTTGGGTCATAACTAGGCGTGGAAACCAGCGCCAGCACCCCAC  >  NC_000913/667108‑667376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑