Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	687,043	A→G	G235G (GGT→GGC)	gltI ←	glutamate/aspartate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	687,043	0	A	G	100.0%	160.8 / NA	46	G235G (GGT→GGC)	gltI	glutamate/aspartate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

TGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAACCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAGGGTGCGGAAA  >  NC_000913/686907‑687178                                                                                                                                         |                                                                                                                                        tGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCg                                                                                                                                      <  2:858069/140‑1 (MQ=255)     cAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAgg                                                                                                                                  >  1:144163/1‑140 (MQ=255)     cAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAgg                                                                                                                                  <  2:30952/140‑1 (MQ=255)      aGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGc                                                                                                                                 <  1:823909/140‑1 (MQ=255)       ggTTTTTCGGCGGAACTGGATTTTTCAACCACTTATCTAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGcc                                                                                                                                <  1:267184/140‑1 (MQ=255)            ttCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTg                                                                                                                           >  2:256878/1‑140 (MQ=255)             tCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTATTGAACTGGGGATCATGTTTACGCAGCATAGAGCCGTAGGCCTCCTga                                                                                                                          >  2:267184/1‑140 (MQ=255)                           tttttGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGcc                                                                                                            <  2:772556/140‑1 (MQ=255)                              ttGAACCACTTATCAAATCATTTTTCCGCTTTACCCGAGTTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCcgac                                                                                                         <  2:385004/140‑1 (MQ=255)                                    cACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTc                                                                                                   >  2:899954/1‑140 (MQ=255)                                        tATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTTCGGCTTGCCGACGATTTCCCAg                                                                                               >  1:385004/1‑140 (MQ=255)                                         aTCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGt                                                                                              >  2:776168/1‑140 (MQ=255)                                           cAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCcgacga                                                                                                       >  2:851130/1‑129 (MQ=255)                                           cAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCcgacga                                                                                                       <  1:851130/129‑1 (MQ=255)                                             aaCCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACCTACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTc                                                                                          <  2:622931/140‑1 (MQ=255)                                              aCCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCt                                                                                         >  1:629052/1‑140 (MQ=255)                                                 aTTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTAGCAGACGATTTCCCAGTTGTCTGGt                                                                                      >  1:270696/1‑140 (MQ=255)                                                     ttCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTttct                                                                                  <  1:899954/140‑1 (MQ=255)                                                        cGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCg                                                                               >  2:135590/1‑140 (MQ=255)                                                        cGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCg                                                                               >  2:201110/1‑140 (MQ=255)                                                                      tCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCa                                                                 <  1:760008/140‑1 (MQ=255)                                                                           aCCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGc                                                            <  1:135590/140‑1 (MQ=255)                                                                           aCCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGc                                                            <  1:201110/140‑1 (MQ=255)                                                                               gAGCGATGGTGTCATCCGTCAGCTTTTTGAATTGCTGATTATCTTTACACAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCcagc                                                        <  2:405550/140‑1 (MQ=255)                                                                               gAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCcagc                                                        <  1:256878/140‑1 (MQ=255)                                                                                          tCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTcatc                                             <  1:776168/140‑1 (MQ=255)                                                                                           catccatcAGCTTTTTGAACTGTGGATCATCCTTACGCACCATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTcatcc                                            <  2:270696/140‑1 (MQ=255)                                                                                                 tcAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGGTTCTTCGCTTTCACACCTTCACCGGGCAGCAGAGCGTCATCCATCATa                                      >  2:391355/1‑140 (MQ=255)                                                                                                  cAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTAGCACGTTAACCCGCCAGCAGACCGTCATCCATCATaa                                     >  1:412716/1‑140 (MQ=255)                                                                                                     cTTTTTGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAgg                                  <  2:140394/140‑1 (MQ=255)                                                                                                        tttGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCCCTTTCGCAAGTTGACCAGCCAGCAGTGCGTCATCCATCATAAAGGCaa                               >  2:358184/1‑140 (MQ=255)                                                                                                         ttGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCGGAACGTCATGCATCATAAAGGCAAc                              >  1:487275/1‑140 (MQ=255)                                                                                                         ttGAACTGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAAc                              <  1:66073/140‑1 (MQ=255)                                                                                                               tGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGc                                 <  2:412633/131‑1 (MQ=255)                                                                                                               tGCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGc                                 >  1:412633/1‑131 (MQ=255)                                                                                                                gCGGATCATCTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGa                       >  2:987446/1‑140 (MQ=255)                                                                                                                         cTTTACGCAACATACTGCCGTACGCGTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTcc              <  1:330305/140‑1 (MQ=255)                                                                                                                         cTTTACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTcc              <  1:987446/140‑1 (MQ=255)                                                                                                                           ttACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAg            <  2:8891/140‑1 (MQ=255)                                                                                                                           ttACGCAACATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGACAGCAGAGCGTCGTCCCTCATAAAGGCAACCGCACGACCGCTTTCCAg            >  2:330305/1‑140 (MQ=255)                                                                                                                             aCGCAACATACAGCCGTAGGCCTCCGGAGCCTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAggg          <  1:391355/140‑1 (MQ=255)                                                                                                                                   cATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAGGGTGCGGa    >  2:139246/1‑140 (MQ=255)                                                                                                                                   cATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAGGGTGCGGa    >  2:622903/1‑140 (MQ=255)                                                                                                                                   cATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAGGGTGCGGa    <  2:629052/140‑1 (MQ=255)                                                                                                                                   cATACAGCCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAGGGTGCGGa    >  2:936090/1‑140 (MQ=255)                                                                                                                                     tACAGCAGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAGGGTGCGGaaa  <  1:964073/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TGTTCAGGTTTTTCGGCGGAATTGGATTTTTGAACCACTTATCAAACCATTTTTCCGCTTCACCGGAGGTCTGCACCTGAGCGATGGTGTCATCCATCAGCTTTTTGAACTGCGGATCATCTTTACGCAACATACAACCGTAGGCCTCCTGAGACTGCGGCTTGCCGACGATTTCCCAGTTGTCTGGTTTCTTCGCTTTCGCACGTTCACCGGCCAGCAGAGCGTCATCCATCATAAAGGCAACGGCACGACCGCTTTCCAGGGTGCGGAAA  >  NC_000913/686907‑687178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑