Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,878	C→T	R17H (CGT→CAT)	thiQ ←	thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,878	0	C	T	100.0%	288.0 / NA	82	R17H (CGT→CAT)	thiQ	thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/45);  total (37/45)

CTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTG  >  NC_000913/72744‑73009                                                                                                                                       |                                                                                                                                    cTACGCCATCGATTGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATc                                                                                                                                >  1:775409/1‑140 (MQ=255)  tACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGTCGCGTTCGACCGGGAAGCTAAAATGCATCg                                                                                                                               >  2:473010/1‑140 (MQ=255)  tACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCg                                                                                                                               >  2:3570/1‑140 (MQ=255)  tACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCg                                                                                                                               >  1:887687/1‑140 (MQ=255)  tACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCg                                                                                                                               >  1:885860/1‑140 (MQ=255)     gCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCa                                                                                                                            <  1:1003056/140‑1 (MQ=255)     gCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCAGCTGGGCGCGTTCCACAGTTAGGCTAAAATGCATCGGCa                                                                                                                            >  2:459401/1‑140 (MQ=255)      ccATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCaa                                                                                                                           >  2:785537/1‑140 (MQ=255)       cATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTCCTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCaaa                                                                                                                          <  1:575165/140‑1 (MQ=255)        aTCGATAGTCAGCGATCCGCTGGCTAGCGGCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAt                                                                                                                         <  1:428548/140‑1 (MQ=255)        aTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAt                                                                                                                         <  2:598904/140‑1 (MQ=255)         tCGATAGTCAGCGAACTACTGGGTGGCGTCAAAAACCCGCCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAtg                                                                                                                        <  2:470122/140‑1 (MQ=255)         tCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAtg                                                                                                                        >  1:95733/1‑140 (MQ=255)         tCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAtg                                                                                                                        <  2:887687/140‑1 (MQ=255)         tCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAtg                                                                                                                        >  2:717277/1‑140 (MQ=255)         tCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAtg                                                                                                                        <  1:628710/140‑1 (MQ=255)          cGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAAtgg                                                                                                                       >  1:739255/1‑140 (MQ=255)              aGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATggtggt                                                                                                                   <  2:885860/140‑1 (MQ=255)              aGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATggtggt                                                                                                                   >  1:100522/1‑140 (MQ=255)              aGTCAGCGAACCGCTGGCTGGCCTCAGAAAAACGGAGATTAAATTCAGAAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATggtggt                                                                                                                   <  2:260671/140‑1 (MQ=255)               gTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTa                                                                                                                  <  2:940057/140‑1 (MQ=255)                  aGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCGCCGTTAAGCTATAATGCATCGACAAATGGTGGTAAAg                                                                                                               >  1:470122/1‑140 (MQ=255)                  aGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAg                                                                                                               >  1:867127/1‑140 (MQ=255)                   gCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGc                                                                                                              <  2:538426/140‑1 (MQ=255)                    cGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATggtggt                                                                                                                   <  2:840499/134‑1 (MQ=255)                    cGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATggtggt                                                                                                                   >  1:840499/1‑134 (MQ=255)                    cGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGcc                                                                                                             >  2:96053/1‑140 (MQ=255)                       aCCGCTGGCTGGCGTCGGAAAACCGGCGATCTAATTCAGTAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAgg                                                                                                          <  2:465369/140‑1 (MQ=255)                       aCCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGTCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCaaa                                                                                                                          <  1:774283/124‑1 (MQ=255)                       aCCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGTCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCaaa                                                                                                                          >  2:774283/1‑124 (MQ=255)                       aCCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAgg                                                                                                          <  2:739255/140‑1 (MQ=255)                       aCCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAgg                                                                                                          <  2:576337/140‑1 (MQ=255)                       aCCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAgg                                                                                                          <  2:562223/140‑1 (MQ=255)                            tggctggcGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGata                                                                                                     >  1:839037/1‑140 (MQ=255)                            tggctggcGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGata                                                                                                     >  2:998159/1‑140 (MQ=255)                              gctggcGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAGAATGCATGGGCGAAGGGTGGTTAAGCCAGGTGATAtc                                                                                                   >  1:267345/1‑140 (MQ=255)                                tggcGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATAtcag                                                                                                 <  1:644189/140‑1 (MQ=255)                                 ggcGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATAtcagt                                                                                                <  2:45039/140‑1 (MQ=255)                                  gcGTCAGAAAACCGGTGATCAAGTTCAGCTGGGTACTGTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATAtcagtc                                                                                               <  1:473010/140‑1 (MQ=255)                                  gcGTCAGAAAACCGGCGATCAACTTCAGCAGCGTAATTTCACCCGCGCGGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATAtcagtc                                                                                               <  2:267345/140‑1 (MQ=255)                                   cGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAATATGGATCGGCATATGATGTTAAAGCGAGGTGATAtcagtca                                                                                              >  1:326149/1‑140 (MQ=255)                                        gAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTCAAATGCATTGGCAAATAGTGGTAAATCCAGAGGATATCAGTCAGtttt                                                                                         >  2:346334/1‑140 (MQ=255)                                               ggCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACAttt                                                                                  >  2:33666/1‑140 (MQ=255)                                                 cGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCg                                                                                <  2:95733/140‑1 (MQ=255)                                                       aaTTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCgg                                                                          <  2:717205/140‑1 (MQ=255)                                                         ttCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTa                                                                        <  2:867127/140‑1 (MQ=255)                                                         ttCAGCAGGGTACTTTCACCCGTGCCGCTTGGCGCTAGGGTCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTa                                                                        <  2:459302/140‑1 (MQ=255)                                                              cagGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGtttt                                                                   >  1:19195/1‑140 (MQ=255)                                                                  gTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTCACATTTCGCCCCGGTAGTTTTTCaa                                                               >  1:466332/1‑140 (MQ=255)                                                                   tACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAAt                                                              <  1:785537/140‑1 (MQ=255)                                                                    aCTTTTACCCGCGCCGCTTGGCACGAGGATCTCCATCTGGTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATc                                                             <  2:466332/140‑1 (MQ=255)                                                                       tttACCCGCGCCGCTTGGCCCGAGGGTCGCCACCTGCCCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACg                                                          <  1:459401/140‑1 (MQ=255)                                                                          ccccGCGCCGCTTGGCCCGAGGTTCGCCACCTGCTTGCCGCGTTCCACAGTTAAGCTAATATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTa                                                       <  2:326149/139‑1 (MQ=255)                                                                          aCCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTa                                                       <  2:839037/140‑1 (MQ=255)                                                                           cccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGGCCGGGTAGTGTTTCAATCACGGTaa                                                      >  1:494633/1‑140 (MQ=255)                                                                           cccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTaa                                                      >  2:540731/1‑140 (MQ=255)                                                                           cccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTaa                                                      >  1:557117/1‑140 (MQ=255)                                                                            ccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTaaa                                                     <  2:110166/140‑1 (MQ=255)                                                                            ccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTaaa                                                     <  2:769790/140‑1 (MQ=255)                                                                            ccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTaaa                                                     >  1:620046/1‑140 (MQ=255)                                                                            ccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCACGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTaaa                                                     <  1:261640/140‑1 (MQ=255)                                                                              gcgcCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAAca                                                   <  1:33666/140‑1 (MQ=255)                                                                               cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCGGTCAGTTTTAACGTTTGGCCCCGGTGGTTTATCAATCACGGTAAAcag                                                  >  2:261640/1‑140 (MQ=255)                                                                               cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAAcag                                                  >  1:988386/1‑140 (MQ=255)                                                                               cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCAGTAGTTTTTAAATCACGGTAAAcag                                                  >  1:354849/1‑140 (MQ=255)                                                                               cgccgcTTGGCCCGAGGATAGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAAcag                                                  >  2:69186/1‑140 (MQ=255)                                                                                gccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAAcagc                                                 >  2:615969/1‑140 (MQ=255)                                                                                 ccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAAcagca                                                <  1:717277/140‑1 (MQ=255)                                                                                 ccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAAcagca                                                <  2:775409/140‑1 (MQ=255)                                                                                                  cGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagcagcagc                               >  1:574372/1‑140 (MQ=255)                                                                                                        cTGCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAAtt                         >  2:79471/1‑140 (MQ=255)                                                                                                          gCTCGCCGCGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTaa                       >  1:646293/1‑140 (MQ=255)                                                                                                              gccgcGTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAAcgcg                   <  1:615969/140‑1 (MQ=255)                                                                                                                  cgTTCCACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGa               <  2:620046/140‑1 (MQ=255)                                                                                                                       cACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCa          <  2:646293/140‑1 (MQ=255)                                                                                                                       cACCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCa          <  1:3570/140‑1 (MQ=255)                                                                                                                        aCCGTTAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCAc         <  2:988386/140‑1 (MQ=255)                                                                                                                            ttAAGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGCGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTc     >  2:99846/1‑140 (MQ=255)                                                                                                                               aGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCa          >  1:567212/1‑132 (MQ=255)                                                                                                                               aGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCa          <  2:567212/132‑1 (MQ=255)                                                                                                                               aGCTAAAATGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTg  <  2:574372/140‑1 (MQ=255)                                                                                                                                    aaaTGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGc           <  1:421134/126‑1 (MQ=255)                                                                                                                                    aaaTGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGc           >  2:421134/1‑126 (MQ=255)                                                                                                                                    aaaTGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTc     <  1:234478/132‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    CTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTG  >  NC_000913/72744‑73009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑