Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	710,089	G→A	W100* (TGG→TGA)	chiQ →	lipoprotein ChiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	710,089	0	G	A	98.2%	181.3 / NA	54	W100* (TGG→TGA)	chiQ	lipoprotein ChiQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/30);  minor base T (0/1);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCG  >  NC_000913/709953‑710216                                                                                                                                         |                                                                                                                                aCGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAg                                                                                                                              <  1:90539/140‑1 (MQ=255)    tGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGaa                                                                                                                           <  2:675560/140‑1 (MQ=255)     gCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGaaa                                                                                                                          >  1:69658/1‑140 (MQ=255)      cTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCTGGCTGTGGAAGCCGATTGTGAGAAAGTCTGACAGGAAAt                                                                                                                         >  2:283665/1‑140 (MQ=255)       tGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGCAATGATCACGCGGTGAAAGCCGATTGTGATCAAGTCTGACAGGAAATa                                                                                                                        >  2:419580/1‑140 (MQ=255)       tGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATa                                                                                                                        <  1:612003/140‑1 (MQ=255)       tGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATa                                                                                                                        <  1:936880/140‑1 (MQ=255)        gaagaaAGAGAAACATCATCAGGAGTTTGACGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATAc                                                                                                                       <  1:372822/140‑1 (MQ=255)        gaagaaAGAGAAACAACTTCAGTAGTTTGCCGAGCAGGATAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATAc                                                                                                                       <  2:445006/140‑1 (MQ=255)         aagaaagaGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACg                                                                                                                      <  2:746647/140‑1 (MQ=255)             aagaGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGt                                                                                                                  <  1:902022/140‑1 (MQ=255)               gagaAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGtaa                                                                                                                <  1:811064/140‑1 (MQ=255)               gagaAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGtaa                                                                                                                <  1:884875/140‑1 (MQ=255)                      aacaTCAGCAGTTTGCCGACCATGACAGTCTACGCGTCCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACaaa                                                                                                         <  1:283665/140‑1 (MQ=255)                            agcagTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTg                                                                                                   >  2:613150/1‑140 (MQ=255)                             gcagTTTGCCGACCAGGAAAGTTTACGCGTACTGGATTACCAGCAGTGCCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTga                                                                                                  <  2:374044/140‑1 (MQ=255)                                  ttGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCt                                                                                             >  2:773506/1‑140 (MQ=255)                                        aCCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAAct                                                                                       >  1:925681/1‑140 (MQ=255)                                           aGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACtctc                                                                                    >  1:992217/1‑140 (MQ=255)                                               aaGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCtt                                                                                <  2:69658/140‑1 (MQ=255)                                               aaGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCtt                                                                                <  2:79269/140‑1 (MQ=255)                                                aGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCttt                                                                               <  2:856575/140‑1 (MQ=255)                                                 gtgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCtttt                                                                              <  2:992217/140‑1 (MQ=255)                                                 gtgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCtttt                                                                              <  1:975303/140‑1 (MQ=255)                                                    tACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCtt                                                                           >  1:772472/1‑140 (MQ=255)                                                      cgcgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAt                                                                         <  2:760528/140‑1 (MQ=255)                                                          tACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTcc                                                                     <  2:145307/140‑1 (MQ=255)                                                           aCTGGATTATCAGCAGTCTCTGCGTGCAACGGAAACCGGTAATGATCATGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTccc                                                                    <  2:351709/140‑1 (MQ=255)                                                            cTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCt                                                                   >  1:767079/1‑140 (MQ=255)                                                                         agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGc                                                      >  2:214357/1‑140 (MQ=255)                                                                             tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGGGAGCTGTATCTCGCGCATTTCTTATGTCCCGTGCATAAAAAAGCCAAc                                                  >  2:271835/1‑140 (MQ=255)                                                                             tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTATCTCTCCCCTTTCTTATTTCCCAAGCATAAAAAAGCCAAc                                                  >  2:454605/1‑140 (MQ=255)                                                                             tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                                                  >  1:988832/1‑140 (MQ=255)                                                                                gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCg                                               <  2:769493/140‑1 (MQ=255)                                                                                      aaCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                         >  1:806467/1‑140 (MQ=255)                                                                                       aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTg                                        >  2:788012/1‑140 (MQ=255)                                                                                         gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc                                      <  2:988832/140‑1 (MQ=255)                                                                                             aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                  <  2:879676/140‑1 (MQ=255)                                                                                                   aaTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGtt                            <  2:233924/140‑1 (MQ=255)                                                                                                      gATCAGGCGGTGAAAGCCGATTTTGATAAAGACTGTCAGTAAATACGCCGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAg                         <  2:273928/140‑1 (MQ=255)                                                                                                          aGGCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                     <  1:773506/140‑1 (MQ=255)                                                                                                           ggCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctgg                    >  2:1019918/1‑140 (MQ=255)                                                                                                            gCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggc                   <  2:772472/140‑1 (MQ=255)                                                                                                            gCGGTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTGGCATGAAAAAGCTAACGCGCAGGTTGGCTATTCTTGTTGGggctggc                   >  1:625583/1‑140 (MQ=255)                                                                                                              ggTGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCtt                 <  2:806467/140‑1 (MQ=255)                                                                                                                tGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAt               <  2:549507/140‑1 (MQ=255)                                                                                                                tGAAAGCCGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAt               >  2:129814/1‑140 (MQ=255)                                                                                                                      ccGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCt         >  2:995353/1‑140 (MQ=255)                                                                                                                      ccGATTGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCt         >  1:503025/1‑140 (MQ=255)                                                                                                                          ttGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt     >  2:776491/1‑140 (MQ=255)                                                                                                                          ttGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt     >  2:728922/1‑140 (MQ=255)                                                                                                                          ttGTGATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGt     >  2:535024/1‑140 (MQ=255)                                                                                                                            gtgATAAAGTCTGACAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc   <  1:214357/140‑1 (MQ=255)                                                                                                                             tgATAAAGTCTGACATGAAATACGCTGTACTAACAAACAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcg  <  1:271835/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                ACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCG  >  NC_000913/709953‑710216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑