Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	768,123	A→G	Y49C (TAT→TGT)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	768,123	0	A	G	98.0%	171.9 / NA	49	Y49C (TAT→TGT)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (24/24);  minor base T (1/0);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACC  >  NC_000913/767989‑768256                                                                                                                                       |                                                                                                                                      aTCTCGCGTTCACATCCTCCCGGATATGCACGGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACg                                                                                                                                  <  2:440296/140‑1 (MQ=255)          tCACATCACCCCGCATATGCACTGGGATCGATAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACg                                                                                                                         >  1:91549/1‑140 (MQ=255)           cacaTCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGCAACAGGACAACGAATACAAATGTTACGTACTCGActg                                                                                                                        >  1:985784/1‑138 (MQ=255)            acaTCACCCCGCATATGTACTGTGATCGAGTTTTGGATTTCACCACCGTAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACggg                                                                                                                       <  2:486646/140‑1 (MQ=255)                  ccccGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACg                                                                                                                 >  1:816877/1‑140 (MQ=255)                  ccccGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTAC‑CGACGGGCAAACgg                                                                                                                <  1:169908/140‑1 (MQ=255)                    ccGCATATGCACTGGAATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGc                                                                                                               <  2:75569/140‑1 (MQ=255)                           tGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTc                                                                                                        <  1:567372/140‑1 (MQ=255)                             cACTGGGATCGAGAGTGGTATCTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATTTCACGTACTCGACGGGTAAACGGCGATCCTCGa                                                                                                      >  2:496386/1‑140 (MQ=255)                                        agagTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATc                                                                                                           >  1:713825/1‑124 (MQ=255)                                        agagTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATc                                                                                                           <  2:713825/124‑1 (MQ=255)                                            tGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCTACGGGCAGACGACGGTCCTCGAAGATTATTTCGCGGt                                                                                       >  1:265508/1‑140 (MQ=255)                                              gTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCg                                                                                                       <  1:577353/122‑1 (MQ=255)                                              gTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCg                                                                                                       >  2:577353/1‑122 (MQ=255)                                                aTTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGaaa                                                                                   <  2:571671/140‑1 (MQ=255)                                                    caccacCGAAGAGTCGCGTATTTTGCTGGTCAATAATCTGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                               <  1:340201/140‑1 (MQ=255)                                                    caccacCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                               <  2:87480/140‑1 (MQ=255)                                                    caccacCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                               <  1:811292/140‑1 (MQ=255)                                                        accGAAGAGTCACGTATTCTGTTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGaaaa                                                                           >  2:226549/1‑140 (MQ=255)                                                            aaGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGaa                                                                             <  1:894413/134‑1 (MQ=255)                                                            aaGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGaa                                                                             >  2:894413/1‑134 (MQ=255)                                                                gTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGAcc                                                                   <  2:780949/140‑1 (MQ=255)                                                                 tCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                               >  2:756743/1‑127 (MQ=255)                                                                 tCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCgg                                                                               <  1:756743/127‑1 (MQ=255)                                                                         tCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCGTCGAAGATTATTTCGCGGTGAAACCAGAAAACAAAGACCGTGTgaaga                                                          >  2:339030/1‑140 (MQ=255)                                                                             ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACa                                                      >  1:47054/1‑140 (MQ=255)                                                                             ctgGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACa                                                      >  2:637692/1‑140 (MQ=255)                                                                                gTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGt                                                   >  1:9294/1‑140 (MQ=255)                                                                                          tGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGc                                         >  1:952039/1‑140 (MQ=255)                                                                                               gagaTCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGtt                                    >  1:722920/1‑140 (MQ=255)                                                                                                  aTCCTGTGGCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGCGTGAAGAAACAGGTAGAAGCCGGCAAGTTGAt                                 >  2:841033/1‑140 (MQ=255)                                                                                                  aTCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGAt                                 >  2:236122/1‑140 (MQ=255)                                                                                                  aTCCTGTGCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGAt                                 >  2:746434/1‑140 (MQ=255)                                                                                                         gCCGACTGGAACAGGACCACGACTACAAATGCTACCTACTCGACGGGCCAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGc                          <  2:265508/140‑1 (MQ=255)                                                                                                         gCCGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGAGTGCAGAAACAGGTAGAAGCCCGCAAGTTGATTATCGGc                          >  2:433696/1‑140 (MQ=255)                                                                                                           cGACTGGAACAGGACGACGAATACACATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                        <  1:433696/140‑1 (MQ=255)                                                                                                           cGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                        <  2:985784/140‑1 (MQ=255)                                                                                                           cGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                        <  2:47054/140‑1 (MQ=255)                                                                                                           cGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                        <  2:91549/140‑1 (MQ=255)                                                                                                           cGACTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGccc                        <  1:226549/140‑1 (MQ=255)                                                                                                             aCTGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCt                      >  2:725055/1‑140 (MQ=255)                                                                                                               tGGAACAGGACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTgg                    <  2:536760/140‑1 (MQ=255)                                                                                                                       gACAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCa            >  1:689164/1‑140 (MQ=255)                                                                                                                         cAACGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGa          >  2:854679/1‑140 (MQ=255)                                                                                                                          aaCGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGAc         <  2:9294/140‑1 (MQ=255)                                                                                                                            cGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATAcc              >  2:877323/1‑133 (MQ=255)                                                                                                                            cGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATAcc              <  1:877323/133‑1 (MQ=255)                                                                                                                            cGAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCg       >  1:104938/1‑140 (MQ=255)                                                                                                                             gAATACAAATGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGa      <  1:854679/140‑1 (MQ=255)                                                                                                                                 aCAAATGTTACGTACTCGACGGGCACACGACGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATAcc  <  1:496386/140‑1 (MQ=255)                                                                                                                                    aaTGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGAcc        <  1:510148/131‑1 (MQ=255)                                                                                                                                    aaTGTTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGAcc        >  2:510148/1‑131 (MQ=255)                                                                                                                                       |                                                                                                                                      ATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTATTTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGACTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAAGATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGCAAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACC  >  NC_000913/767989‑768256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑