Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	783,280	C→T	L39F (CTT→TTT)	pnuC →	nicotinamide riboside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	783,280	0	C	T	100.0%	104.1 / NA	32	L39F (CTT→TTT)	pnuC	nicotinamide riboside transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/28);  total (4/28)

ACGTTACGCTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCCTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACG  >  NC_000913/783145‑783415                                                                                                                                        |                                                                                                                                        aCGTTACGCTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGc                                                                                                                                     <  1:110413/140‑1 (MQ=255) aCGTTACGCTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGc                                                                                                                                     <  1:664730/140‑1 (MQ=255) aCGTTACGCTTTGGGCGAAAGACGGCTTTATTTAGTGCGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGc                                                                                                                                     <  1:278489/140‑1 (MQ=255)   gTTACGCTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCa                                                                                                                                   <  1:913401/140‑1 (MQ=255)     tACGCTTTGGGGGAAACATGGAGTTTTTTAGTGTGCAGATTATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGt                                                                                                                                 <  2:466280/140‑1 (MQ=255)      aCGCTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTc                                                                                                                                >  2:533016/1‑140 (MQ=255)      aCGCTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTc                                                                                                                                <  1:610034/140‑1 (MQ=255)         cTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTgg                                                                                                                             <  1:533016/140‑1 (MQ=255)             gggggAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGagaa                                                                                                                         >  2:870681/1‑140 (MQ=255)                    aGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATGGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGc                                                                                                                  <  1:139985/140‑1 (MQ=255)                         gATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGGTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAacta                                                                                                             >  2:859007/1‑140 (MQ=255)                                   gtgtGCAGAATATCCTGATACAAATACCAATAGGGTCAGACGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGc                                                                                                   <  2:329880/140‑1 (MQ=255)                                   aggtgCAGAATATCCGGGTACAAATGCCAGTAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGc                                                                                                   <  1:340344/138‑1 (MQ=255)                                        cAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGAt                                                                                              <  1:870681/140‑1 (MQ=255)                                            atatCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAAc                                                                                          <  1:845869/140‑1 (MQ=255)                                              atCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGt                                                                                        <  1:97219/140‑1 (MQ=255)                                                ccTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCa                                                                                      >  1:62123/1‑140 (MQ=255)                                                ccTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACCATCGCCCGGCTGCTGTGTATTGGGTTTAGCAGTCTGAAGGAGATGAGCAACTAATTCTGTGGGCTGATCAACGTcc                                                                                      >  2:340344/1‑139 (MQ=255)                                                ccTGGTACATATACCAATAGGGGCAGGCGGCTATTATCTCTGATGGGCCGGAACGGCAAGCACCAGCGCCGGGGTGGTGGGTATTGACTTTGCCCGTCTTGAGAAGATTAGCAACAAATTCTTTTGCCTGATCAACGTcc                                                                                      >  2:1014268/1‑139 (MQ=255)                                                agacagaCATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCa                                                                                      <  1:26670/134‑1 (MQ=255)                                                     tACATATACCAATAGGGGCAGGCGGTTATGATCTCTTATGGATCGAAGCGGTAGGCAAGATCGCCGGGTTGCTGTGTATTGGCTTTTCCCGTCTGGAGAAGATCTGCAGATACTTCTTTTGGGTGATCAACGTCACCttt                                                                                 >  2:383015/1‑139 (MQ=255)                                                       cATATACCAATAGGGGCAGGCGGTTATTATCTCTCATGGATCGAAACGGGAGGCACGATCGCCGGGGTGCTGTGTATTTGCTTTTCCAGTCTGGAGAAAATCAGCAAATACTTCTTTTGCCCGATCAACGTCACCCTGtt                                                                               >  2:247160/1‑140 (MQ=255)                                                         tataCCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGGTGCTGTGTATTGGCTTTGCCCGTCTTGAGAAGATCAGCAACTACTTCTTTTGCCTGATCAACGTCCCCTTGTTTg                                                                             >  2:2827/1‑140 (MQ=255)                                                          ataCCAATAGGGGCAGGCGGTTATGATCTCTCATGGATTGAAGCGGTAGGCACGATCGCCCGGGTGCTGTGGATTGGCTTTTGCCGGCTGGAGAAGATCAGCAACTACTTTTTTGGCCTGATGAACGTCGCCCTTTTTgg                                                                            >  2:194367/1‑140 (MQ=255)                                                           taCCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGGAGGCACGATCGCCGGGGTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTTGCCTGATCAACGTCACCTTGttttgg                                                                           >  2:693774/1‑137 (MQ=255)                                                               aaTAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCGGTGTATTGGCTTTGCCCGTCTGGAGAAGATCAGCAACTACTTCTTTTGCCTGATCAAAGTCACCTTGTTTTGCattt                                                                       >  2:634130/1‑139 (MQ=255)                                                                 tAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGGGGTAGGCACGATTGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCCGCAACTACTTCTTTGGGCTGATTAACGTCACCTTGTTTTGcctggct                                                                     >  2:26670/1‑134 (MQ=255)                                                                    gggCAGGCGGTTATGATCTCTCATGGATCGAAGCGGGAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTAttttt                                                                  >  1:205455/1‑139 (MQ=255)                                                                      gCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATtttctt                                                                <  1:133333/140‑1 (MQ=255)                                                                       cAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATtttcttt                                                               <  1:634130/140‑1 (MQ=255)                                                                       cAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATtttcttt                                                               <  1:607974/140‑1 (MQ=255)                                                                             gTTATGATCTCTCATGGATCGACGCGGTATGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGAtt                                                         <  1:383015/140‑1 (MQ=255)                                                                             gTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGAtt                                                         <  1:240454/140‑1 (MQ=255)                                                                               tATGATCTCTCATGGATCGAAGCGGTAGGCACG‑TCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGa                                                           >  2:866968/1‑135 (MQ=255)                                                                               tATGATCTCTCATGGATCGAAGCGGTAGGCACG‑TCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGa                                                           <  1:866968/135‑1 (MQ=255)                                                                                aTGATCTCTCATGGATCGAAGCTGTAGGCCCGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAg                                                      <  1:478082/140‑1 (MQ=255)                                                                                  gATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCt                                                    <  1:210735/140‑1 (MQ=255)                                                                                           tGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAg                                           <  1:693774/140‑1 (MQ=255)                                                                                              aTCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGACTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCt                                        <  1:1014268/140‑1 (MQ=255)                                                                                                cGAAGCGGTAGGCACTATCGCCTGGATGCTGTGTATTTGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGc                                      <  1:475310/140‑1 (MQ=255)                                                                                                  aaGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGCTa                                    >  1:938932/1‑140 (MQ=255)                                                                                                                           gCTGTGTATTGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGa           <  2:19470/140‑1 (MQ=255)                                                                                                                                    tGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGTCGCGAATATTTACg  <  1:757998/140‑1 (MQ=255)                                                                                                                                    tGGCTTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACg  <  2:938932/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ACGTTACGCTTTGGGGGAAAGATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCAGGCGGTTATGATCTCTCATGGATCGAAGCGGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCCTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCAACGTCACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACG  >  NC_000913/783145‑783415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑