Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	864,274	G→A	W211* (TGG→TGA)	fsaA →	fructose‑6‑phosphate aldolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	864,274	0	G	A	100.0%	152.7 / NA	45	W211* (TGG→TGA)	fsaA	fructose‑6‑phosphate aldolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/26);  total (19/26)

TTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCG  >  NC_000913/864139‑864405                                                                                                                                        |                                                                                                                                    tttCAAAACCCCGCGCCAGGCGCTGCACTTCTTACTGCCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAgg                                                                                                                                 <  1:285816/140‑1 (MQ=255)   tCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGa                                                                                                                               <  2:32176/140‑1 (MQ=255)      aaaCCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCg                                                                                                                            <  2:603140/140‑1 (MQ=255)           ccGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGCTGATGCCGCTGTGGAGAAGTTTGAGGAGGAATGACAGGGAGCGTTTgg                                                                                                                       >  2:500366/1‑140 (MQ=38)            cgcgTCAGGCGCTGGGCTGCTTACTGGCACGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGc                                                                                                                      <  1:449133/140‑1 (MQ=255)              cgTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAg                                                                                                                    >  1:959671/1‑140 (MQ=255)              cgTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAg                                                                                                                    >  2:677321/1‑140 (MQ=255)                  aGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACg                                                                                                                <  1:963082/140‑1 (MQ=255)                         ggACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGAttt                                                                                                         >  1:505054/1‑140 (MQ=255)                               cTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCgg                                                                                                   <  1:839513/140‑1 (MQ=255)                                ttACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGa                                                                                                  <  2:763594/140‑1 (MQ=255)                                         ggATGTGAATCAATTGCTCTGCTACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCa                                                                                         <  1:471382/140‑1 (MQ=255)                                         ggATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCa                                                                                         <  2:79748/140‑1 (MQ=255)                                         ggATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCa                                                                                         <  2:760819/140‑1 (MQ=255)                                         ggATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCa                                                                                         >  1:833897/1‑140 (MQ=255)                                         ggATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAACGTTTGGGAGATCGACGATTTGACCGGACGTTCTGCa                                                                                         >  1:478938/1‑140 (MQ=255)                                                        aCTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCt                                                                          >  1:61858/1‑140 (MQ=255)                                                            tGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCtgatg                                                                      >  2:1012379/1‑140 (MQ=255)                                                              ccACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGAc                                                                    >  2:690760/1‑140 (MQ=255)                                                              ccACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGAc                                                                    >  1:542767/1‑140 (MQ=255)                                                               cACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACg                                                                   <  2:237152/140‑1 (MQ=255)                                                                    gATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCg                                                              >  1:562176/1‑140 (MQ=255)                                                                     aTGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCgg                                                             <  2:505054/140‑1 (MQ=255)                                                                          gCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGc                                                        <  2:183799/140‑1 (MQ=255)                                                                              aacaGATGATTAGCTATCCGGCTGTTGATTCCGCTGTCGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCcgcg                                                    <  1:490665/140‑1 (MQ=255)                                                                                caGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACATGGCGGAGTGCCGCGTc                                                  >  2:322976/1‑140 (MQ=255)                                                                                    tgatTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTAt                                              <  2:763095/140‑1 (MQ=255)                                                                                    tgatTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTAt                                              <  2:693882/140‑1 (MQ=255)                                                                                    tgatTAGCTATCCGGCGGTTGACGCCGCTCTGGCGAATTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTAt                                              <  1:353385/140‑1 (MQ=255)                                                                                            tATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTg                                      >  1:155372/1‑140 (MQ=255)                                                                                             aTCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTgg                                     >  2:726457/1‑140 (MQ=255)                                                                                             aTCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTgg                                     <  2:959671/140‑1 (MQ=255)                                                                                             aTCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTgg                                     <  2:229632/140‑1 (MQ=255)                                                                                             aTCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTgg                                     <  2:109875/140‑1 (MQ=255)                                                                                              tCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATc                                             <  2:752172/131‑1 (MQ=255)                                                                                              tCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATc                                             >  1:752172/1‑131 (MQ=255)                                                                                                   cggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTgg                               <  1:38129/140‑1 (MQ=255)                                                                                                    ggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAAGTGCTGATGGCGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                              >  2:335667/1‑140 (MQ=255)                                                                                                           gCCGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACt                       <  2:562176/140‑1 (MQ=255)                                                                                                             cGCTGTGGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGc                     >  1:526332/1‑140 (MQ=255)                                                                                                                tgtgGCGACGTTTGAGACGGACTGACAGGGAACGTTTGGGAGAACGTCCATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCca                  <  2:478938/140‑1 (MQ=255)                                                                                                                tgtgGCGAAGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCca                  >  1:789819/1‑140 (MQ=255)                                                                                                                    gcaaaGTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCcacaca              >  2:645876/4‑140 (MQ=255)                                                                                                                         gTTTGAGCAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTca         <  2:526332/140‑1 (MQ=255)                                                                                                                                cAGGACTGACAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCg  <  2:61858/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCG  >  NC_000913/864139‑864405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑