Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	921,427	A→G	N361D (AAC→GAC)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	921,427	0	A	G	100.0%	128.2 / NA	38	N361D (AAC→GAC)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/26);  total (12/26)

TCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGA  >  NC_000913/921291‑921560                                                                                                                                         |                                                                                                                                      tCCCGGGAAAGATTTTGGCTATGACGATCCGCAATATCAGCAGGCGCGGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACa                                                                                                                                    >  2:652315/1‑140 (MQ=255)    cGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAatg                                                                                                                                 >  1:992362/1‑140 (MQ=255)      ggAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAatgtt                                                                                                                               >  1:162083/1‑140 (MQ=255)      ggAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAatgtt                                                                                                                               >  1:59208/1‑140 (MQ=255)      ggAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAatgat                                                                                                                               >  2:900268/1‑138 (MQ=255)       gAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAatgttg                                                                                                                              >  2:901907/1‑140 (MQ=255)                  gCGATGACGATCCGCAATATCACCAAGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGcc                                                                                                                   <  1:405216/140‑1 (MQ=255)                    gATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAg                                                                                                                 <  1:34287/140‑1 (MQ=255)                      tGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTg                                                                                                               >  1:188156/1‑140 (MQ=255)                        aCGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGcc                                                                                                             <  1:694402/140‑1 (MQ=255)                         cGATCCGCAATATCAACAGGCGTTGTAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCa                                                                                                            <  1:366324/140‑1 (MQ=255)                                      cagcagGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAgcgg                                                                                               <  1:610623/140‑1 (MQ=255)                                         cagGCGCTGAAGTACGAGGACTCAATCGCCATCAATAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGa                                                                                            <  2:455116/140‑1 (MQ=255)                                         cagGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGa                                                                                            <  1:652315/140‑1 (MQ=255)                                         cagGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGa                                                                                            <  1:900989/140‑1 (MQ=255)                                         cagGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGa                                                                                            <  2:188156/140‑1 (MQ=255)                                         cagGCGCTGAAGTACCACGACTTAATCGCTATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGa                                                                                            <  1:459251/140‑1 (MQ=255)                                          agGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGat                                                                                           <  2:162083/140‑1 (MQ=255)                                           ggCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGatt                                                                                          >  1:73067/1‑140 (MQ=255)                                            gcgcTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGatta                                                                                         <  1:846811/140‑1 (MQ=255)                                                 gAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTa                                                                                    <  1:718628/140‑1 (MQ=255)                                                 gAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTa                                                                                    <  2:152773/140‑1 (MQ=255)                                                       cgacgaCTTAATCGCCATCCAAAAACAACCGAGGGCCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGCCAATGTTGATGTTGCTACCAGTGCCAATGGCGTGAGCGGCGGTTATTTTAATgctt                                                                              >  2:900989/1‑137 (MQ=255)                                                            acTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGc                                                                         <  2:99632/140‑1 (MQ=255)                                                                    gCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCtt                                                                 <  2:73067/140‑1 (MQ=255)                                                                      cATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCa                                                               >  1:186497/1‑140 (MQ=255)                                                                               acaacCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGaa                                                      <  1:458715/140‑1 (MQ=255)                                                                                 aacCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAac                                                    <  1:873173/140‑1 (MQ=255)                                                                                 aacCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAac                                                    <  1:212377/140‑1 (MQ=255)                                                                                   ccGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAacac                                                  <  2:579743/140‑1 (MQ=255)                                                                                   ccGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAacac                                                  >  2:870140/1‑140 (MQ=255)                                                                                             ccTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGACGATTATTTTAATGTCTGTGGCATGGCCTGCAATGAAGGTAACACCTTTAATCAg                                        >  1:290455/1‑140 (MQ=255)                                                                                                cAGCCACACCTGCCGTCTCGCATAACCTGCGTCTGGGTTGTGACAATGCTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGag                                     <  2:443249/140‑1 (MQ=255)                                                                                                        ccTGCCGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCAGCTGaa                             <  2:186497/140‑1 (MQ=255)                                                                                                             cGTCTCGCAAAACCTGCGCCTGCGTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGGGAAGGAAACACATTTAATCAGGAGCAGCTGAACGGTc                        >  1:449488/1‑140 (MQ=255)                                                                                                                         cctgcgcctgcgTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCg            <  1:900268/140‑1 (MQ=255)                                                                                                                              gcctgcgTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGtt       <  1:870140/140‑1 (MQ=255)                                                                                                                                 tgcgTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTtctc    <  1:901907/140‑1 (MQ=255)                                                                                                                                   cgTTATGACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGa  <  1:85233/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGACTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTAATGTCTATGGCATGACCTTCAGTGAAGGAAACACCTTTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGA  >  NC_000913/921291‑921560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑