| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 81,515 | C→T | Q148Q (CAG→CAA) | leuB ← | 3‑isopropylmalate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 81,515 | 0 | C | T | 98.0% | 154.7 / ‑4.2 | 49 | Q148Q (CAG→CAA) | leuB | 3‑isopropylmalate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (24/24); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01 | |||||||||||
TCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGA > NC_000913/81378‑81645 | tcgatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTg > 2:752031/1‑140 (MQ=255) cgatcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGa < 2:599566/140‑1 (MQ=255) tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGGTTTTTCATGTTGACCGCTACCTTCGCGGCCTTTTGGTTGACCg > 1:628906/1‑140 (MQ=255) tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTTTCCGCTACCTTCGCGGCCTTTTGGTTGACCg > 1:1007252/1‑140 (MQ=255) tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCg > 1:893453/1‑140 (MQ=255) tcgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTAATATTGTTCGCGACGTTCGCGGCCTTTTGGTTGACCg > 1:302259/1‑140 (MQ=255) cgaCGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGa < 1:7786/140‑1 (MQ=255) aCTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTGTTCATATTGTCCCCTAGCTTCGCGGACTTTAGGTTGACCGAAATAgct > 2:349600/1‑138 (MQ=255) aCGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCa > 2:216154/1‑140 (MQ=255) cGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAg < 1:21774/140‑1 (MQ=255) tGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTcg < 1:216154/140‑1 (MQ=255) cGAGCAGATTCAAACTCGATGCTGGTGATCCGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTcgcg < 1:316857/140‑1 (MQ=255) cGAGCAGATTCAAACGCGATGCGGGCGATACTTTCTATCACAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTcgcg < 2:412828/140‑1 (MQ=255) cAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGcaca < 1:672429/140‑1 (MQ=255) cAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGcaca > 1:885757/1‑140 (MQ=255) gATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGcacaca < 2:851438/140‑1 (MQ=255) tCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAg > 1:246536/1‑140 (MQ=255) gATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGa > 2:782146/1‑140 (MQ=255) tGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAg < 1:596233/140‑1 (MQ=255) tACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCg > 1:117916/1‑140 (MQ=255) gTTCGATCTCAAAACGGTGATATACCTCGGTTTCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCa < 2:441622/140‑1 (MQ=255) ttCGATCTCAAAACGGTTATACTCCTCGGTATCAACGGCCTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCaa < 2:430128/140‑1 (MQ=255) tcAAAACGGTGATACACTTCGGTATCAAAGGTTTTGTCAAATTGTCCGTTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGc < 2:346906/140‑1 (MQ=255) aCGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCa < 2:793806/140‑1 (MQ=255) aCGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCa < 2:893453/140‑1 (MQ=255) cGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAg < 1:835210/140‑1 (MQ=255) tGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCgg > 1:849931/1‑140 (MQ=255) tGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAACCGTTTTCGGCAAAGTCTGCACGCAGCgg > 1:444337/1‑140 (MQ=255) atgccccTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACCCAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGAc < 1:744693/135‑1 (MQ=255) acCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGaa < 2:516914/140‑1 (MQ=255) cGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGTCAACGTCTGCACGCAGCGGACAGAATGCt > 1:383652/1‑140 (MQ=255) aTCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCa < 2:885757/140‑1 (MQ=255) cAAAGGCTTTTTCATATTGTCCTCTACCTTCGCGGCCCTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGc < 1:275355/140‑1 (MQ=255) cAAAGGCTTTTTCATATAGTCCTCTACCTTCACGGACTTCTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGc < 2:417631/140‑1 (MQ=255) aaGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGTCGTTTGCGGCAATGTCTGCACGCAGCGGa < 1:241854/123‑1 (MQ=255) aaGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGccc < 2:117916/140‑1 (MQ=255) aaGGCTTTTTCATATTGTCCGCTACCTTCGCGCCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGTCGTTTGCGGCAATGTCTGCACGCAGCGGa > 2:241854/1‑123 (MQ=255) aGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGcccc < 1:958616/140‑1 (MQ=255) tttttCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAATCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGAt > 1:221870/1‑140 (MQ=255) tttttCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGAt > 1:11529/1‑140 (MQ=255) tttCATATTGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTGTGCACGCAGCGGACAGAATGCGTCCAGCCCCTGATAc > 1:417631/1‑140 (MQ=255) tGTCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGc > 2:229412/1‑140 (MQ=255) tCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCg > 1:574343/1‑140 (MQ=255) tCCGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCg > 2:916672/1‑140 (MQ=255) cGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCggg > 2:535499/1‑140 (MQ=255) cGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCggg > 2:827431/1‑140 (MQ=255) cGCTACCTTCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCggg > 1:191524/1‑140 (MQ=255) tCGCGGCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGtt > 1:501798/1‑140 (MQ=255) gCCTTTTGGTTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGa < 1:916672/140‑1 (MQ=255) | TCGATCGACGTCACTTTGTGGCGACGCTTGCGAGCAGATTCAAACGCGATGCGGGCGATACGTTCGATCTCAAAACGGTGATACACCTCGGTATCAAAGGCTTTTTCATATTGTCCGCTACCTTCGCGGCCTTTTGGCTGACCGAAATAGATGCCGCCGGTCAGTTCGCGCACACACAGGATGTCGAAGCCGTTTGCGGCAATGTCTGCACGCAGCGGACAGAATGCTTCCAGCCCCTGATACAGTTTTGCCGGGCGCAGGTTGCTGA > NC_000913/81378‑81645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |