Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	942,977	T→A	D673E (GAT→GAA)	dmsA →	dimethyl sulfoxide reductase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	942,977	0	T	A	100.0%	110.7 / NA	35	D673E (GAT→GAA)	dmsA	dimethyl sulfoxide reductase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/21);  total (14/21)

CGTTGCTTATAAAGCCTTCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTG  >  NC_000913/942842‑943106                                                                                                                                        |                                                                                                                                  cGTTGCTTATAAAGCCTTCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCGACCTGGGAATTGCCGGAAGGCGATGTGATCGAACCAc                                                                                                                               >  1:459004/1‑140 (MQ=255)     gCTTATAAAGCCTTCCTTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGcc                                                                                                                           >  2:874364/1‑140 (MQ=255)        tataAAGCCTTCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGAt                                                                                                                        <  2:552206/140‑1 (MQ=255)                cTTCCGTGAAGATCCGGGGGCACACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGc                                                                                                                <  2:459004/140‑1 (MQ=255)                cTTCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGc                                                                                                                >  1:665500/1‑140 (MQ=255)                   ccGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCgg                                                                                                             >  1:541189/1‑140 (MQ=255)                   ccGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCgg                                                                                                             >  1:59623/1‑140 (MQ=255)                          gATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGa                                                                                                      <  2:852740/140‑1 (MQ=255)                             ccGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAg                                                                                                   <  2:909175/140‑1 (MQ=255)                              cGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCAACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGACGGGCTTTGAAAGt                                                                                                  >  2:962282/1‑140 (MQ=255)                                     aaaCCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAgg                                                                                           >  1:130518/1‑140 (MQ=255)                                       aCCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGAt                                                                                         <  2:22346/140‑1 (MQ=255)                                        cccACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATc                                                                                        >  2:548949/1‑140 (MQ=255)                                           aCTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGTTCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGc                                                                                     <  1:101304/140‑1 (MQ=255)                                               aCTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGaa                                                                                 <  2:198053/140‑1 (MQ=255)                                                           ggCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATcc                                                                     >  1:533452/1‑140 (MQ=255)                                                               aaaTTGAGATTTATTCGCGGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCATGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTg                                                                 <  2:160154/140‑1 (MQ=255)                                                               aaaTTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGTCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTg                                                                 <  2:930230/140‑1 (MQ=255)                                                               aaaTTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTg                                                                 <  2:627884/140‑1 (MQ=255)                                                               aaaTTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTg                                                                 <  1:761174/140‑1 (MQ=255)                                                                      gaTTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTa                                                          <  2:59623/140‑1 (MQ=255)                                                                      gaTTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTa                                                          <  1:548949/140‑1 (MQ=255)                                                                      gaTTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTa                                                          <  1:734649/140‑1 (MQ=255)                                                                            ttCGCAGGCGCTGGCTGACATTGCCACTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTA‑AGGttt                                                   <  2:780011/140‑1 (MQ=255)                                                                                          ctgACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAAtct                                      <  2:778929/140‑1 (MQ=255)                                                                                             aCATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGTCTTTGAAAGTTATCAGGATCCGCTTAACAAACATTATACACGGCAGCTTAGAGGTGACCACTATAAATCTcgc                                   >  2:494677/1‑140 (MQ=255)                                                                                             aCATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTcgc                                   <  1:68296/140‑1 (MQ=255)                                                                                                    cGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACt                            <  1:600676/140‑1 (MQ=255)                                                                                                        aCCTGGGAATTGCCTTAAGGCGCTGTGATCGAACCACCGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAAc                        <  1:376261/140‑1 (MQ=255)                                                                                                              gAATTGCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACgg                  <  2:665500/140‑1 (MQ=255)                                                                                                              gAATTGCCTGAAGGCCATGTGACCGCACCACTGCCGAACTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACgg                  <  1:494677/140‑1 (MQ=255)                                                                                                                   gCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACg             >  1:674257/1‑140 (MQ=255)                                                                                                                   gCCTGAAGGCGATGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACg             >  1:240252/1‑140 (MQ=255)                                                                                                                              aTGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTg  >  1:663119/1‑140 (MQ=255)                                                                                                                              aTGTGATCGAACCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTg  >  2:572385/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  CGTTGCTTATAAAGCCTTCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTG  >  NC_000913/942842‑943106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑