Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,062,228	A→G	T144A (ACC→GCC)	torD →	trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,062,228	0	A	G	100.0%	78.1 / NA	24	T144A (ACC→GCC)	torD	trimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/10);  total (14/10)

TAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGC  >  NC_000913/1062110‑1062353                                                                                                                       |                                                                                                                              tAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCg                                                                                                          >  1:145828/1‑140 (MQ=255)         tGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTGAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTg                                                                                                  >  1:879698/1‑140 (MQ=255)               ttGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCaa                                                                                            <  1:233221/140‑1 (MQ=255)                        ggATGGAAACCAGAGGCAATTTCAACGAAACGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTg                                                                                   >  2:727752/1‑140 (MQ=255)                            ggAAACCAGCGGCAAGTTCAACTAACCGGTAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACgg                                                                               <  1:464591/140‑1 (MQ=255)                              aaaCCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGgcg                                                                             >  2:615816/1‑140 (MQ=255)                                      ggCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGTCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCa                                                                     <  1:826348/140‑1 (MQ=255)                                            ttCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGtt                                                               <  1:507239/140‑1 (MQ=255)                                                        gCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACAGCGCTGTGGCAATTGTTACCAGAGTttgt                                                   >  1:374963/1‑140 (MQ=255)                                                           gATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTgc                                                <  1:615816/140‑1 (MQ=255)                                                                   ggCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgtc                                        >  2:704204/1‑140 (MQ=255)                                                                      gATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTgtcgtc                                     <  1:727752/140‑1 (MQ=255)                                                                                  aTTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTGTGACAGCt                         >  1:628819/1‑140 (MQ=255)                                                                                  aTTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCt                         <  1:249313/140‑1 (MQ=255)                                                                                      cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACTAGAGTTTTTTGCGCGTGGTCGTCAGTATGACAGCTTTgg                     >  2:303727/1‑140 (MQ=255)                                                                                      cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTgg                     >  1:759056/1‑140 (MQ=255)                                                                                      cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTgg                     >  2:623513/1‑140 (MQ=255)                                                                                      cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTgg                     >  2:9631/1‑140 (MQ=255)                                                                                      cTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACC‑GAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGt                    >  2:96527/1‑140 (MQ=255)                                                                                              tCTGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTAGCGCGTTATCGACAGTATGACGGCTTTGGTTTTTAcg             >  1:342151/1‑140 (MQ=255)                                                                                                tGCATTTTTCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTAcgcg           >  2:660657/1‑140 (MQ=255)                                                                                                     ttttCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACt      <  1:704204/140‑1 (MQ=255)                                                                                                        tCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAg   <  1:9631/140‑1 (MQ=255)                                                                                                        tCGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAg   <  1:24199/140‑1 (MQ=255)                                                                                                         cGCTGGGAGAGGGGGCCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGc  >  1:708114/1‑140 (MQ=255)                                                                                                                       |                                                                                                                              TAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCGCTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTTTACGCGGCACTAAGC  >  NC_000913/1062110‑1062353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑