Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,108,782	A→G	F54L (TTC→CTC)	opgC ←	protein required for succinyl modification of osmoregulated periplasmic glucans

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,108,782	0	A	G	100.0%	251.5 / NA	70	F54L (TTC→CTC)	opgC	protein required for succinyl modification of osmoregulated periplasmic glucans
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/37);  total (33/37)

TGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAAGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGCCAGGCGCGGATGGAGTCGAG  >  NC_000913/1108647‑1108908                                                                                                                                        |                                                                                                                               tGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCa                                                                                                                            >  1:584786/1‑140 (MQ=255) tGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCa                                                                                                                            >  1:566462/1‑140 (MQ=255)   gCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCAtt                                                                                                                          >  2:143171/1‑140 (MQ=255)           cATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAggg                                                                                                                  <  2:730520/140‑1 (MQ=255)             tCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTc                                                                                                                >  2:181582/1‑140 (MQ=255)                ggATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGc                                                                                                             <  1:891899/140‑1 (MQ=255)                  aTACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCca                                                                                                           <  2:983457/140‑1 (MQ=255)                         cacGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAatgat                                                                                                    >  1:920924/1‑140 (MQ=255)                          acGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAGATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAatgatg                                                                                                   >  2:611187/1‑140 (MQ=255)                             ttCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGCATGAGGTCATTCAATAGGGTCTGCCACAATGATGAtt                                                                                                >  1:477933/1‑140 (MQ=255)                              tCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATATCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTc                                                                                               >  1:827875/1‑140 (MQ=255)                              tCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGGTGAGGTCGTTATAATGGGTCAGCCACAATGATGATTc                                                                                               >  1:349590/1‑140 (MQ=255)                               cGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCg                                                                                              >  1:16128/1‑140 (MQ=255)                               cGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCg                                                                                              <  2:664210/140‑1 (MQ=255)                               cGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCg                                                                                              >  2:87600/1‑140 (MQ=255)                                gACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGAtt                                                                                                <  2:103715/137‑1 (MQ=255)                                gACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGAtt                                                                                                >  1:103715/1‑137 (MQ=255)                                gACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCgg                                                                                             >  1:178389/1‑140 (MQ=255)                                gACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCgg                                                                                             >  1:805919/1‑140 (MQ=255)                                gACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCgg                                                                                             >  1:691529/1‑140 (MQ=255)                                 acacGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGgc                                                                                            <  1:640452/140‑1 (MQ=255)                                  cacGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGgcg                                                                                           <  1:87600/140‑1 (MQ=255)                                       aCTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTAtt                                                                                      >  1:211938/1‑140 (MQ=255)                                       aCTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTAtt                                                                                      >  2:832507/1‑140 (MQ=255)                                         tttCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTca                                                                                    <  2:98466/140‑1 (MQ=255)                                          ttCCACCATTTTTTCAAGGGATTGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTcac                                                                                   <  1:465456/140‑1 (MQ=255)                                          ttCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTcac                                                                                   >  1:535593/1‑140 (MQ=255)                                             caccaTTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATg                                                                                <  1:853421/140‑1 (MQ=255)                                              accaTTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGc                                                                               <  2:178389/140‑1 (MQ=255)                                               ccaTTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGcc                                                                              >  1:1019380/1‑140 (MQ=255)                                               ccaTTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGcc                                                                              >  2:680085/1‑140 (MQ=255)                                               ccaTGTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATTCCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGcc                                                                              <  1:55878/140‑1 (MQ=255)                                                   tttttCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGt                                                                          <  2:845852/140‑1 (MQ=255)                                                   tttttCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGt                                                                          <  2:1019380/140‑1 (MQ=255)                                                   tttttCAAGGGATAGCGAAAAAGAAGCATGTCGGAAAAGCAGCCGGATCTAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGt                                                                          <  2:305246/140‑1 (MQ=255)                                                     tttCAAGGGATAGCGTAAAAAAAGCATGTAGTAAACGTACCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTAt                                                                        <  2:410809/140‑1 (MQ=255)                                                             gATAGCGTAAAAAAAGCTTGTATGAAAAGTAGCCGGATAGAACGAAAACTACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                <  2:349590/140‑1 (MQ=255)                                                             gATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                <  1:181582/140‑1 (MQ=255)                                                             gATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                <  2:535593/140‑1 (MQ=255)                                                             gATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                <  2:566462/140‑1 (MQ=255)                                                             gATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                <  1:565431/140‑1 (MQ=255)                                                              aTAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAAt                                                               >  1:241278/1‑140 (MQ=255)                                                                 gCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGa                                                            <  2:933707/140‑1 (MQ=255)                                                                   gTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTGGGCGCTAGTCACATGCCATGTGTGGCTCGAATAGAtt                                                          >  1:280429/1‑140 (MQ=255)                                                                    tAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTa                                                         <  1:751463/140‑1 (MQ=255)                                                                       aaaaaGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                <  1:353058/130‑1 (MQ=255)                                                                       aaaaaGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAg                                                      >  1:52428/1‑140 (MQ=255)                                                                       aaaaaGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAg                                                      <  1:143171/140‑1 (MQ=255)                                                                       aaaaaGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATAATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                >  2:353058/1‑130 (MQ=255)                                                                         gagacaggTAGGAAATGTAGCCGGATATACCGAAAAAAACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGaa                                                    <  1:449965/133‑1 (MQ=255)                                                                         aaaGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGaa                                                    <  1:620237/140‑1 (MQ=255)                                                                             cATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                >  2:906213/1‑124 (MQ=255)                                                                             cATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGaa                                                                <  1:906213/124‑1 (MQ=255)                                                                              aTGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATg                                               <  1:74916/140‑1 (MQ=255)                                                                               tGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGa                                              <  2:603707/140‑1 (MQ=255)                                                                                gTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGaa                                             <  2:584786/140‑1 (MQ=255)                                                                                    gAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAgg                                         <  2:241278/140‑1 (MQ=255)                                                                                      aaaGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTa                                       <  1:200383/140‑1 (MQ=255)                                                                                         gTAGCCGGATATAACGAGAAATCCCTGCATGGGGACCGACTGGATGAGATCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATcc                                    <  2:424742/140‑1 (MQ=255)                                                                                         gTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATcc                                    >  2:1017313/1‑140 (MQ=255)                                                                                           aGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACTTGCCATGTATGGTTAGAATAGATTAGAGAATGATGAAAAggctgtctc                                  >  2:449965/1‑133 (MQ=255)                                                                                            gCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTa                                 >  2:24297/1‑140 (MQ=255)                                                                                            gCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTa                                 >  2:541954/1‑140 (MQ=255)                                                                                             ccGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTaa                                <  1:1017313/140‑1 (MQ=255)                                                                                                    taACGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATc                         >  1:542753/1‑140 (MQ=255)                                                                                                       cGAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGc                      >  2:722912/1‑140 (MQ=255)                                                                                                        gAAAAATACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGcc                     <  1:213340/140‑1 (MQ=255)                                                                                                           aaaTACCTGCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGCCAgg                  >  1:687128/1‑140 (MQ=255)                                                                                                                 ctcgctGCGCAACGCCTGGATGACATTATTAAAAAGGCTGAGCCACCATGATGATTAGGCGCTCTTCACATGCCATGTATGGCTCGCAAAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGCCAGGCGCGGa            <  2:974938/135‑1 (MQ=255)                                                                                                                   gCATGCGGAACGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGCCAGGCGCGGATg          <  2:752273/140‑1 (MQ=255)                                                                                                                           aaCGAGTGGATGAGGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGCCAGGCGCGGATGGAGTCGAg  >  1:169174/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                               TGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAAAAGCATGTAGGAAAAGTAGCCGGATATAACGAAAAATACCTGCATGCGGAACGAGTGGATGAAGTCATTAAAAAGGGTCAGCCACAATGATGATTCGGCGCTATTCACATGCCATGTATGGCTCGAATAGATTAAAGAAATATGAAAAGGTATCCCTAACAACATCAGCCAGGCGCGGATGGAGTCGAG  >  NC_000913/1108647‑1108908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑