Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	98,181	A→G	V365V (GTA→GTG)	murD →	UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	98,181	0	A	G	100.0%	138.0 / NA	40	V365V (GTA→GTG)	murD	UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/17);  total (23/17)

AAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTACGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGTTCTGCT  >  NC_000913/98045‑98315                                                                                                                                         |                                                                                                                                       aaGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAacgtgcgt                                                                                                                                     <  2:858762/140‑1 (MQ=255)    cGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTg                                                                                                                                  >  1:884463/1‑140 (MQ=255)               ggCAGTACGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCgg                                                                                                                       >  2:174323/1‑140 (MQ=255)                    tACGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTg                                                                                                                  >  2:784393/1‑140 (MQ=255)                     aCGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGa                                                                                                                 <  2:654397/140‑1 (MQ=255)                      cGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATAGCGATAACGTGCGTCTGCATTGTTTCGGTCGTGAc                                                                                                                >  2:459767/1‑140 (MQ=255)                         aaGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGgc                                                                                                             >  1:545470/1‑140 (MQ=255)                              gcgcTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCa                                                                                                        >  2:582820/1‑140 (MQ=255)                              gcgcTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCa                                                                                                        >  1:91160/1‑140 (MQ=255)                                    aaTGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTggc                                                                                                  >  1:978947/1‑140 (MQ=255)                                        gCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGgcggcg                                                                                              <  1:595089/140‑1 (MQ=255)                                          cTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCt                                                                                            <  1:784393/140‑1 (MQ=255)                                           tGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTa                                                                                           <  2:191894/140‑1 (MQ=255)                                                  aGACGGTACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTAGGTCGTGGCGGTGCGCAGCTGGCGGCGCTACGCCCgg                                                                                    >  1:436040/1‑140 (MQ=255)                                                   gACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGa                                                                                   <  2:122527/140‑1 (MQ=255)                                                           acTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAg                                                                           >  1:829912/1‑140 (MQ=255)                                                             tGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGaa                                                                         <  2:711432/140‑1 (MQ=255)                                                               cATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCTCGCAGCTGGCGCCGATACGCCGGGAAGTGGCAGAACa                                                                       >  1:267814/1‑140 (MQ=255)                                                                  ttgttgCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAAc                                                                    <  2:978947/140‑1 (MQ=255)                                                                      tgCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGAAGGCGCGCATCTGGCGGCGCTACGCCCGGAATTGGCAGAACAAACCGaa                                                                >  2:423334/1‑140 (MQ=255)                                                                       gCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGa                                                                 >  2:804405/1‑138 (MQ=255)                                                                       gCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGa                                                                 <  1:804405/138‑1 (MQ=255)                                                                            gTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATg                                                          >  1:561806/1‑140 (MQ=255)                                                                               gCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGaa                                                       >  2:235963/1‑140 (MQ=255)                                                                                     gTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGTACGAACCGATACTATGGAACAGGCg                                                 >  1:421943/1‑140 (MQ=255)                                                                                     gTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCg                                                 <  1:235963/140‑1 (MQ=255)                                                                                     gTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCg                                                 >  2:92529/1‑140 (MQ=255)                                                                                     gTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCg                                                 >  1:512569/1‑140 (MQ=255)                                                                                                   tttAGCCCACTGGCGCGGTACCCGAATGGCGCTAACGTGTGTCTGTATCGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGc                                   <  2:264846/140‑1 (MQ=255)                                                                                                      aGCCCACTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTcc                                <  1:174323/140‑1 (MQ=255)                                                                                                           aCTGGCGCGTTACCTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCgtg                           >  2:99316/1‑140 (MQ=255)                                                                                                                       ccTGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCg               >  1:810165/1‑140 (MQ=255)                                                                                                                         tGAATGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCATGCAGGCTCCGCGTGTTCAGCCGGGCGat             >  1:500763/1‑140 (MQ=255)                                                                                                                          gAATGGCGATAACGTGCGTCTGGATTGTTTCCGTCATGAAGGCGCGCAACTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGata            <  2:379413/140‑1 (MQ=255)                                                                                                                          gAATGGCGATAACGTGCGTCTGAATTGTTTCGGGCGTGACGGCGCGCAACTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGata            <  2:500763/140‑1 (MQ=255)                                                                                                                             tGGCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGTGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATgg         <  1:906145/140‑1 (MQ=255)                                                                                                                               gCGATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGtt       <  2:91160/140‑1 (MQ=255)                                                                                                                                 gATAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACCGGCGATGCACTTGCGGTCTCCGCGTGTTCAACCGGGCGACATGGTTCt     >  2:491189/1‑140 (MQ=255)                                                                                                                                  aTAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGTTCTg    >  2:103668/1‑140 (MQ=255)                                                                                                                                  aTAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGTTCTg    >  2:977568/1‑140 (MQ=255)                                                                                                                                   tAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGTTCTGc   <  2:545470/140‑1 (MQ=255)                                                                                                                                   tAACGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGTTCTGc   <  2:810165/140‑1 (MQ=255)                                                                                                                                    aaCGTGCGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGTTCTGct  <  2:829912/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AAGCGACCAACGTCGGCAGTACGGAAGCGGCGCTGAATGGCCTGCACGTAGACGGCACACTGCATTTGTTGCTGGGTGGCGATGGTAAATCGGCGGACTTTAGCCCACTGGCGCGTTACCTGAATGGCGATAACGTACGTCTGTATTGTTTCGGTCGTGACGGCGCGCAGCTGGCGGCGCTACGCCCGGAAGTGGCAGAACAAACCGAAACTATGGAACAGGCGATGCGCTTGCTGGCTCCGCGTGTTCAGCCGGGCGATATGGTTCTGCT  >  NC_000913/98045‑98315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑