Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,582	C→T	G497D (GGT→GAT)	cvrA ←	putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,582	0	C	T	100.0%	163.3 / NA	47	G497D (GGT→GAT)	cvrA	putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/21);  total (26/21)

CGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAG  >  NC_000913/1240449‑1240715                                                                                                                                      |                                                                                                                                      cGGTGCCGCGCCTAACATCTGCCGGACAATTCCACACAGCGTTTGCTGTTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAaaga                                                                                                                                 <  2:359914/140‑1 (MQ=255) cGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAaaga                                                                                                                                 <  1:519831/140‑1 (MQ=255) cGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAaaga                                                                                                                                 <  2:664068/140‑1 (MQ=255) cGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAaaga                                                                                                                                 >  2:916123/1‑140 (MQ=255)          gcCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGAt                                                                                                                        >  2:829621/1‑140 (MQ=255)                          aCAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACcggcgg                                                                                                        >  1:997153/1‑140 (MQ=255)                              tttCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGAc                                                                                                    >  1:655766/1‑140 (MQ=255)                              tttCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGAc                                                                                                    >  1:1012101/1‑140 (MQ=255)                                   cccAGCGTTTGCTGCTTATCACGATATTCTCCCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCt                                                                                               <  2:377005/140‑1 (MQ=255)                                     cAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGa                                                                                             <  1:985640/140‑1 (MQ=255)                                        cGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACa                                                                                          >  1:73881/1‑140 (MQ=255)                                                       aCGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCg                                                                           <  2:655766/140‑1 (MQ=255)                                                                   cccGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAg                                                                                <  2:466293/123‑1 (MQ=255)                                                                   cccGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGtt                                                               >  1:35547/1‑140 (MQ=255)                                                                   cccGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGtt                                                               >  1:1020061/1‑140 (MQ=255)                                                                   cccGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGGTTACCGAg                                                                                >  1:466293/1‑123 (MQ=255)                                                                     cGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTcc                                                             <  2:997153/140‑1 (MQ=255)                                                                       tCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCg                                                           >  2:697911/1‑140 (MQ=255)                                                                       tCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCg                                                           >  2:604688/1‑140 (MQ=255)                                                                       tCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCg                                                           >  1:936211/1‑140 (MQ=255)                                                                         ttCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAc                                                         <  2:35547/140‑1 (MQ=255)                                                                         ttCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAc                                                         <  2:1012101/140‑1 (MQ=255)                                                                         ttCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAc                                                         <  1:604688/140‑1 (MQ=255)                                                                            cAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCaa                                                      >  2:683573/1‑140 (MQ=255)                                                                              ggCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAAtt                                                    <  2:936211/140‑1 (MQ=255)                                                                                 cATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTaca                                                 <  1:194914/140‑1 (MQ=255)                                                                                    atatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTacacac                                              <  2:611082/140‑1 (MQ=255)                                                                                    atatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTacacac                                              >  1:885668/1‑140 (MQ=255)                                                                                         aGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACacacaacac                                         >  2:512194/1‑140 (MQ=255)                                                                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAc                                                         >  2:765127/1‑122 (MQ=255)                                                                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAc                                                         <  1:765127/122‑1 (MQ=255)                                                                                             cAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCg                                     >  1:177827/1‑140 (MQ=255)                                                                                             cAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGAGTTCCCTACCAATTACACACAACACATCg                                     >  1:352755/1‑140 (MQ=255)                                                                                                cATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCt                                  <  2:9484/140‑1 (MQ=255)                                                                                                cATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCAAGCGCCGGGAGAACGCGTTCCCGACAAATTACACACAACACATCGCCt                                  >  2:312874/1‑140 (MQ=255)                                                                                                   cAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTcg                               <  2:73881/140‑1 (MQ=255)                                                                                                       atatTTGGCGCTGGCTTCGAGACTGCAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCa                           <  1:312874/140‑1 (MQ=255)                                                                                                              gcgcTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGt                    >  1:48340/1‑140 (MQ=255)                                                                                                                gcTGGCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGGGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGtgc                  >  2:191127/1‑140 (MQ=255)                                                                                                                   ggCTTCGAGAATGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAGTTAGACACAACACATCACCTTAGCGCAGTCGGGtgctgc               >  2:329445/1‑140 (MQ=255)                                                                                                                              tGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACAGATCGCCTCCGCACAGTCGGGGACTGCCGGTAGGATGa    >  1:480000/1‑140 (MQ=255)                                                                                                                              tGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTCGGATGa    >  2:997671/1‑140 (MQ=255)                                                                                                                              tGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATg     >  1:1001600/1‑139 (MQ=255)                                                                                                                              tGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATg     <  2:1001600/139‑1 (MQ=255)                                                                                                                              tGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGa    <  2:48340/140‑1 (MQ=255)                                                                                                                              tGAAGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGa    >  1:884897/1‑140 (MQ=255)                                                                                                                                aaGTCATCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAg  <  1:916123/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAACACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAG  >  NC_000913/1240449‑1240715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑