Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,583	A→G	V77A (GTA→GCA)	ychF ←	redox‑responsive ATPase YchF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,583	0	A	G	100.0%	105.4 / NA	30	V77A (GTA→GCA)	ychF	redox‑responsive ATPase YchF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

TTAACTTTGCCGGAAACGTGAATGATGTTGTCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAA  >  NC_000913/1257454‑1257718                                                                                                                                  |                                                                                                                                        ttAACTTTGCCGGAAACGTGAATGATGTTGTCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGc                                                                                                                               >  1:64649/1‑140 (MQ=255)           cGGAAACGTGAATGATGTTGTCTTTTTCAAATCAGCGAAGAACGTGACTGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACa                                                                                                                     <  2:274986/140‑1 (MQ=255)              aaaCGTGAATGATGTTGTCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTCCGATGCGCCTTATGCCAGACCAGCGATATCGACAAAt                                                                                                                  >  1:424232/1‑140 (MQ=255)                  gTGAATGATGTTGTCGTTTTCACAGCAGCGAACAACGCGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCa                                                                                                              <  1:374284/140‑1 (MQ=255)                  gTGAATGATGTTGTCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCa                                                                                                              <  2:64649/140‑1 (MQ=255)                  gTGAATGATGTTGTCATCTTCACAGCAGCGACCAATGTGCCCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAAACCGGCGATATCGACAAATTCCa                                                                                                              <  2:304449/140‑1 (MQ=255)                      atgatgTTGTCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGt                                                                                                          <  2:150637/140‑1 (MQ=255)                            ttgtCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTgg                                                                                                     >  1:664035/1‑139 (MQ=255)                            ttgtCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTgg                                                                                                     <  2:664035/139‑1 (MQ=255)                                   tttCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTa                                                                                             >  2:1810/1‑140 (MQ=255)                                    ttCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTCTTGCCAGATCGGAGATAACGACAAATTCCATAGTCGTGGGAAGCGTAc                                                                                            >  2:473740/1‑140 (MQ=255)                                              cGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGTCAAATTCAATGATCTTGGGAAGCGTACGCTGCGGttt                                                                                  >  1:397357/1‑140 (MQ=255)                                              cGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACAGGCGATATGGACGAATACCATGGTCCTGGGAAGCGTACGCTGCGGttt                                                                                  >  2:386078/1‑140 (MQ=255)                                                aacaacGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTa                                                                                <  1:192081/140‑1 (MQ=255)                                                aacaacGTGACCGATCGCTTCGCTTTCACGGATGTTGATCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTa                                                                                <  1:386078/140‑1 (MQ=255)                                                         aCCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAg                                                                       <  1:98828/140‑1 (MQ=255)                                                             aTCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCGGCGGTTTTACGATTTCAGCCAg                                                                   >  1:374843/1‑140 (MQ=255)                                                                  ttCCGTTTCACGGATGTTGGTCAGGAACTGGTTTCCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGATGCGTACGCTGCGGTTTTTCGATTTCAGCCAGTTGAt                                                              <  2:998213/140‑1 (MQ=255)                                                                   tCGGTTTCACGGATGTTTGTCATGAACTGGTCACCCAGAACGTCGCCTATCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATc                                                             <  2:303708/140‑1 (MQ=255)                                                                        ttCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGc                                                        <  2:116186/140‑1 (MQ=255)                                                                         tCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCg                                                       <  2:892700/140‑1 (MQ=255)                                                                               aTGTTGGTCAGGAACCGGTTACCCAAACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGAGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATc                                                 <  1:367634/140‑1 (MQ=255)                                                                                         ggAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGt                                       >  1:27834/1‑140 (MQ=255)                                                                                               ggTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTacgacg                                 >  2:1002163/1‑140 (MQ=255)                                                                                                    cccAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTa                                      >  1:696653/1‑130 (MQ=255)                                                                                                    cccAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTa                                      <  2:696653/130‑1 (MQ=255)                                                                                                    cccAGACCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCtgt                            >  1:935244/1‑140 (MQ=255)                                                                                                         aCCTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCg                       >  2:209948/1‑140 (MQ=255)                                                                                                          ccTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCgg                      >  2:28433/1‑140 (MQ=255)                                                                                                          ccTTCGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCgg                      >  1:706005/1‑140 (MQ=255)                                                                                                              cGCCTTTCGATGCGCCTTTTGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCa                  >  2:624948/1‑140 (MQ=255)                                                                                                                              ttttGCCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGaa  <  1:209948/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        TTAACTTTGCCGGAAACGTGAATGATGTTGTCATTTTCAAAGCAGCGAACAACGTGACCGATCGCTTCGGTTTCACGGATGTTGGTCAGGAACTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAA  >  NC_000913/1257454‑1257718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑