Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,260,656 A→G L50L (TTG→CTG)  dauA ← aerobic C4‑dicarboxylate transporter DauA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,260,6560AG97.4% 132.4 / NA 39L50L (TTG→CTG) dauAaerobic C4‑dicarboxylate transporter DauA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (25/13);  minor base T (1/0);  total (26/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAA  >  NC_000913/1260531‑1260789
                                                                                                                             |                                                                                                                                     
tACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCg                                                                                                                         >  2:33729/1‑140 (MQ=255)
tACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACAACTCCCAATAGCCAGCGCCATCGCCAGCg                                                                                                                         >  1:362712/1‑140 (MQ=255)
  ccACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCggg                                                                                                                       >  2:916364/1‑140 (MQ=255)
        aTGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCa                                                                                                                 >  1:120509/1‑140 (MQ=255)
        aTGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCa                                                                                                                 >  1:1000907/1‑140 (MQ=255)
        aTGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACAACTACCAATAGACATCGCCATAGCCAGCGGGATAGCa                                                                                                                 >  1:266574/1‑140 (MQ=255)
                gTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACTCCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATccc                                                                                                         <  2:586785/140‑1 (MQ=255)
                         gAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTAt                                                                                                >  2:530531/1‑140 (MQ=255)
                           aaCGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATcc                                                                                              >  1:911393/1‑140 (MQ=255)
                                       tGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGt                                                                                  <  1:916364/140‑1 (MQ=255)
                                         acccacccGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCa                                                                                >  1:569722/1‑140 (MQ=255)
                                              cccGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGt                                                                                  >  1:895393/1‑133 (MQ=255)
                                              cccGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGt                                                                                  <  2:895393/133‑1 (MQ=255)
                                                      agCAATGACAATCCCGGCAACATCTTCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTg                                                                   <  1:278801/140‑1 (MQ=255)
                                                          aTGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGc                                                               <  2:911393/140‑1 (MQ=255)
                                                           tGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCa                                                              <  2:505983/140‑1 (MQ=255)
                                                            gACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAg                                                             <  1:528591/140‑1 (MQ=255)
                                                                    ccGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATAtttt                                                     >  1:773233/1‑140 (MQ=255)
                                                                     cGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATAttttt                                                    >  1:664738/1‑140 (MQ=255)
                                                                             cTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGATATCCCGGCAATCAGGTAACGGGCACACCGTGCGGCCGTTTATTTTTCTTTCCAg                                            >  1:444544/1‑140 (MQ=255)
                                                                              tGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGc                                           <  1:556453/140‑1 (MQ=255)
                                                                                  gTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGc                                       <  1:530531/140‑1 (MQ=255)
                                                                                             gTACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTTACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCagag                            >  2:709836/1‑140 (MQ=255)
                                                                                              tACTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAgc                           >  1:710463/1‑140 (MQ=255)
                                                                                               aCTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCg                                      <  1:838908/128‑1 (MQ=255)
                                                                                               aCTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCg                                      >  2:838908/1‑128 (MQ=255)
                                                                                               aCTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCag                              >  1:814977/1‑136 (MQ=255)
                                                                                               aCTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCag                              <  2:814977/136‑1 (MQ=255)
                                                                                               aCTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAgcg                          >  2:644034/1‑140 (MQ=255)
                                                                                               aCTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAgcg                          >  2:936395/1‑140 (MQ=255)
                                                                                               aCTGGGGTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAgcg                          >  2:956200/1‑140 (MQ=255)
                                                                                                   gggTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGa                      <  1:644034/140‑1 (MQ=255)
                                                                                                   gggTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGa                      >  1:607671/1‑140 (MQ=255)
                                                                                                     gTGCCACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGaaa                    <  1:33729/140‑1 (MQ=255)
                                                                                                        ccACACCACTACCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTGTTCTTTCAAGCTAGAGTCGATGAGAGCGCGGAAAGGc                 >  1:260083/1‑140 (MQ=255)
                                                                                                                  aCCAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACAtgtg       >  1:245135/1‑140 (MQ=255)
                                                                                                                   ccAATAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGa      >  1:27816/1‑140 (MQ=255)
                                                                                                                       tAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGaa  >  2:639320/1‑140 (MQ=255)
                                                                                                                       tAGCCAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGaa  >  2:927759/1‑140 (MQ=255)
                                                                                                                          ccAGCGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCTGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCGTCACAtgt        <  2:789939/131‑1 (MQ=255)
                                                                                                                             |                                                                                                                                     
TACCACAAATGCCGCAGTCGGACCGGAAACGCTAAAGCGTGACCCACCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAA  >  NC_000913/1260531‑1260789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑