Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,264,036	A→G	D108G (GAT→GGT)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,264,036	0	A	G	100.0%	126.3 / NA	36	D108G (GAT→GGT)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/15);  total (21/15)

CAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCG  >  NC_000913/1263902‑1264160                                                                                                                                       |                                                                                                                             cAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGTGTGTTGCCAGCGGCCTGGGTTCAc                                                                                                                         >  1:949518/1‑140 (MQ=255)  aaCCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACt                                                                                                                        <  2:107862/140‑1 (MQ=255)      tGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGtt                                                                                                                    >  1:573671/1‑140 (MQ=255)         aaGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCAGTTAATGCGTGTTGCCATCGGCGTGGGTTCACTGGTTCTg                                                                                                                 >  2:269612/1‑140 (MQ=255)              gCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACt                                                                                                                        >  2:585804/1‑128 (MQ=255)              gCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACt                                                                                                                        <  1:585804/128‑1 (MQ=255)              gCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGa                                                                                                            >  2:187807/1‑140 (MQ=255)                              tttGCGATTATCACAATCCTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGt                                                                                            <  2:770541/140‑1 (MQ=255)                                tGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCa                                                                                          >  2:707354/1‑140 (MQ=255)                                tGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCa                                                                                          >  1:931221/1‑140 (MQ=255)                                        tCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTaaaa                                                                                  <  1:577281/140‑1 (MQ=255)                                                      aagaagATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGAGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGAt                                                                    >  2:704952/1‑140 (MQ=255)                                                       agaagaTCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGAtt                                                                   <  1:187807/140‑1 (MQ=255)                                                             tCTTCGTAAAAGCCTCTACTGGCATCAGGATCACGCCGCGGTTAGCCAGTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGAGTCGCaaa                                                             <  2:437036/140‑1 (MQ=255)                                                             tCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGTCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCaaa                                                             <  2:823175/140‑1 (MQ=255)                                                             tCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCaaa                                                             <  1:176728/140‑1 (MQ=255)                                                              cTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCaaaa                                                            <  1:704952/140‑1 (MQ=255)                                                                gCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAg                                                          <  2:749770/140‑1 (MQ=255)                                                                 cGTAAAAGCCTCTACTGGCATCAGGGTAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAgg                                                         >  1:12279/1‑140 (MQ=255)                                                                 cGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGTGGAGCGGCAGATCCGCGGACAGGTTAAAAAATCGTTAGCCGATTCGCAAAAAgg                                                         >  2:288695/1‑140 (MQ=255)                                                                      aaGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCaaaa                                                            >  1:819075/1‑132 (MQ=255)                                                                      aaGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCaaaa                                                            <  2:819075/132‑1 (MQ=255)                                                                           tctACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAg                                               >  1:206864/1‑140 (MQ=255)                                                                                  gCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCACAAAAAGGTGATATGAAGGCCAGCg                                        >  1:431099/1‑140 (MQ=255)                                                                                       aGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTg                                   >  2:518801/1‑140 (MQ=255)                                                                                             acgacgCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGc                             >  2:533663/1‑140 (MQ=255)                                                                                                acgCGGTTAGCCATTCAATGCGCGTCGCCCGCGGCCTCGGTTCACTGGTTCTGGTGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATg                          <  1:288695/140‑1 (MQ=255)                                                                                                   cgGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAACAGGTCATGTGACGGGCAGCGAACTGGAACGCATGTTc                       >  1:330510/1‑140 (MQ=255)                                                                                                   cgGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTc                       >  1:746588/1‑140 (MQ=255)                                                                                                   cgGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTc                       >  2:519816/1‑140 (MQ=255)                                                                                                    ggTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTcc                      <  1:200554/140‑1 (MQ=255)                                                                                                     gTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTCCa                     >  1:671360/1‑140 (MQ=255)                                                                                                                aaTGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTtct          <  2:535585/140‑1 (MQ=255)                                                                                                                aaTGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCGGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTtct          <  2:400847/140‑1 (MQ=255)                                                                                                                aaTGCGTGTTGCCAGAGGCCTGTGTTCACTGGTTCTGCGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTtct          <  1:338070/140‑1 (MQ=255)                                                                                                                  tGCGTGTTGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGGCATATGAAGGCCCGCGAACTGGAACACATGTTCCAGAAATCTTTctct        >  2:338070/1‑140 (MQ=255)                                                                                                                        ttGCCAGCGGCCTGGGTTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTcgcg  >  2:878507/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                             CAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCATGTTCCAGAAATCTTTCTCTGTCGCG  >  NC_000913/1263902‑1264160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑