| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,270,445 | T→C | noncoding (53/68 nt) | rdlC → | small regulatory antisense RNA RdlC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,270,445 | 0 | T | C | 97.9% | 141.1 / NA | 47 | noncoding (53/68 nt) | rdlC | small regulatory antisense RNA RdlC |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (31/15); total (31/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCA > NC_000913/1270309‑1270580 | cGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCCTATTGCCGCCTTCTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAAcgcgcg < 2:441792/140‑1 (MQ=255) gCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGcca < 2:547377/140‑1 (MQ=17) gTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGccacc < 1:818680/140‑1 (MQ=17) gccgccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATaa > 1:563253/1‑140 (MQ=17) ccgccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATaaa > 2:740139/1‑140 (MQ=17) cgccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAg > 1:934814/1‑140 (MQ=17) ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGcc > 1:147967/1‑140 (MQ=17) ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGcc > 1:198711/1‑140 (MQ=17) ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGcc > 2:659625/1‑140 (MQ=17) ttgttgTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCCGCAACCCATGCCAGCAGGGATAAAGcccc > 1:355858/1‑140 (MQ=255) ttgttgTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGcccc > 1:953305/1‑140 (MQ=17) tgttgtAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCg < 2:217452/140‑1 (MQ=18) acaacaTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTgcg < 2:552630/140‑1 (MQ=37) caacaTTTTGCGGCTATGCTTGAAGTGTCTGGTTTGAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTgcgc < 2:349283/140‑1 (MQ=255) aacaTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGcc > 1:646740/1‑140 (MQ=37) caTTTTGGGGCTATTCTTGTAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTc > 2:587968/1‑140 (MQ=255) ttttGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCATGGAGAATGCCCCGTAACATTGCGCCTCAc > 1:469858/1‑140 (MQ=11) ttttGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCAc > 2:105818/1‑140 (MQ=37) cTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAAc > 1:723064/1‑140 (MQ=37) cTATTCTTGAAGCGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAAc > 1:822596/1‑140 (MQ=255) tATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAAcc > 1:583245/1‑139 (MQ=255) ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCggt < 2:563253/140‑2 (MQ=255) gtgtCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGgt < 1:1021462/140‑1 (MQ=255) gtCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGgtgt > 2:133102/1‑140 (MQ=255) gtCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCACAACATTACGCCTCACCGGATAACGCCGGCTTGgtgt > 1:375047/1‑140 (MQ=255) tCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGCTAAAGCCCCGCAGCCTCGCGCCTCAACGGATCACGCCGGCTTGGtgtg > 2:443669/1‑140 (MQ=14) tCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGtgtg > 2:874239/1‑140 (MQ=255) tCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGtgtg > 2:622012/1‑140 (MQ=255) tCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGtgtg > 1:876703/1‑140 (MQ=255) tCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGtgtg > 1:764861/1‑140 (MQ=255) tCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGAAACATTGGGCCTCATCGGATAACGCCGGCTTGGtgtg > 1:298619/1‑140 (MQ=255) aGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGt < 1:243428/140‑1 (MQ=255) aGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGt > 1:588783/1‑140 (MQ=255) aGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGAATAACGCCGGCTTGGTGTGGATACTACGt > 1:459519/1‑140 (MQ=255) gATTAGCCCCCGTTTTGTTGTCCGGTTTTACCTCTTAACCCGCGGGGGCTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTc < 2:375047/140‑1 (MQ=14) aGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTAACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 2:404470/1‑140 (MQ=255) gCCCCCGTTTTGTTGTCAGGTTTTACCTCTCGACGTGCGTGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAt < 2:459519/140‑1 (MQ=255) cccccGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAtt > 2:17118/1‑140 (MQ=255) cGTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc > 2:250620/1‑140 (MQ=255) gTTTTGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATct < 1:133102/140‑1 (MQ=255) ttttGTTGTCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATctt < 1:42330/140‑1 (MQ=255) ttgtCAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt < 2:583245/140‑1 (MQ=255) tgtCAGGTTTTACCTCTCAACGCGCGGGGGTATTCCCTTTCCAGCAACTAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcacttc < 2:298619/140‑1 (MQ=255) cAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcacttca > 2:167082/1‑138 (MQ=255) cAGGTTTTACCTCTCAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcacttca < 1:167082/138‑1 (MQ=255) tcAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 2:215573/1‑140 (MQ=255) tcAACGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 2:851845/1‑140 (MQ=255) aaCGCGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa < 1:832071/140‑1 (MQ=255) | CGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCA > NC_000913/1270309‑1270580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |