Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,308,423	A→G	W75R (TGG→CGG)	clsA ←	cardiolipin synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,308,423	0	A	G	100.0%	136.1 / NA	38	W75R (TGG→CGG)	clsA	cardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

TTCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCG  >  NC_000913/1308290‑1308555                                                                                                                                      |                                                                                                                                     ttCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGc                                                                                                                                >  2:26064/1‑140 (MQ=255) ttCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGc                                                                                                                                >  2:823759/1‑140 (MQ=255)  tCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGct                                                                                                                               <  2:696107/140‑1 (MQ=255)               aGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGATGGCCGCATCACTCTGGCGCGCTCAg                                                                                                                  >  1:342964/1‑140 (MQ=255)                     ccccTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAGGCCATTTTGCGGTGGAGGGCCGCATGGCTCTGGCGCGCTCAGCgcggc                                                                                                            >  2:408535/1‑140 (MQ=255)                                     cAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAg                                                                                            <  2:863948/140‑1 (MQ=255)                                           ttGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGcc                                                                                      <  1:857015/140‑1 (MQ=255)                                                    ggCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAg                                                                             >  1:201572/1‑140 (MQ=255)                                                    ggCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAg                                                                             >  1:112199/1‑140 (MQ=255)                                                     gcgcAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGa                                                                            <  1:856179/140‑1 (MQ=255)                                                       gcAGCGACCCTGCTATCTTCTTCGGCGAAGGAAGGCTTAGAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATa                                                                          <  2:479313/140‑1 (MQ=255)                                                       gcAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATa                                                                          <  2:3668/140‑1 (MQ=255)                                                            gACACTGCTATTTTCTTCGGCGTAGATATGCCTACTGGCTTTAAGGTCTTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCaa                                                                     <  2:389193/140‑1 (MQ=255)                                                                cTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGACTAAATGGAGCTCGTCAACGGTAAGATAGGCaataat                                                                 >  1:479313/1‑140 (MQ=255)                                                                        ttcttcGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTa                                                         >  1:546913/1‑140 (MQ=255)                                                                             cGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCAAAATGGAGGTCGGCAAGGGCAAGATGGGCAGTAATTCCGACTAACGGc                                                    >  2:273078/1‑140 (MQ=255)                                                                             cGGCGAAGATATGCTTACAGGCTTTAAGGTAGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGc                                                    <  1:6136/140‑1 (MQ=255)                                                                             cGGCGAAGATATGCTTACAGGCTTTAAGGTAGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGc                                                    >  2:6136/1‑140 (MQ=255)                                                                             cGGCGAAGATATGCTCACAGGCCTTTAGGCCGTTAAGACATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGc                                                    <  1:273078/140‑1 (MQ=255)                                                                              ggCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCa                                                   <  2:948062/140‑1 (MQ=255)                                                                               gCGAAGATATGCTTACATGCTTAAAGGTCGTCAAGCCATCTTGCGGTGCAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAg                                                  <  2:267812/140‑1 (MQ=255)                                                                                cGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGa                                                 <  2:201572/140‑1 (MQ=255)                                                                                            tACAGGCTTTAAGGTCGTTAAGACACTTTTCGGTGGACGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATc                                     <  1:327245/140‑1 (MQ=255)                                                                                             aCAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCa                                    >  1:822443/1‑140 (MQ=255)                                                                                             aCAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCa                                    <  1:809948/140‑1 (MQ=255)                                                                                               aGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAAc                                  >  2:939412/1‑140 (MQ=255)                                                                                               aGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAAc                                  >  1:43776/1‑140 (MQ=255)                                                                                               aGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACCGCAAGATACGCAATAAGTCCGACTACCGGCGGAATGTAAATAATCAAc                                  >  2:323055/1‑140 (MQ=255)                                                                                                   tttAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCACTAAATCCTACTAACGGCAGAATGTAAATAATCAACAGcc                              >  2:457594/1‑140 (MQ=255)                                                                                                     tAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATc                                     >  1:676060/1‑131 (MQ=255)                                                                                                     tAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATc                                     <  2:676060/131‑1 (MQ=255)                                                                                                      aaGGTCGTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAgg                           <  1:26064/140‑1 (MQ=255)                                                                                                            gTTAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcg                     >  1:906634/1‑140 (MQ=255)                                                                                                             ttAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcgc                    <  2:546913/140‑1 (MQ=255)                                                                                                             ttAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcgc                    <  2:873863/140‑1 (MQ=255)                                                                                                             ttAAGCCATTTTGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATcgc                    <  1:939412/140‑1 (MQ=255)                                                                                                                      tttGCGGTGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATATGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAAc           >  1:790542/1‑140 (MQ=255)                                                                                                                             tGGAAGGCCGCATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGcgt    <  2:855792/140‑1 (MQ=255)                                                                                                                               gAAGGCCGCATCGCTCTGGCGCCCTTAGCCCGGCGTTAGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGcgtcg  <  1:323055/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     TTCCCTTTGACCCCAGCGATCCCCTGACGACGCTCGCAAAGCTTGAATAATGGCGCAGCGACACTGCTATTTTCTTCGGCGAAGATATGCTTACAGGCTTTAAGGTCGTTAAGCCATTTTGCGGTGGAAGGCCACATCGCTCTGGCGCGCTCAGCGCGGCGTTTGCCTAAATGGAGCTCGCCAACGGCAAGATAGGCAATAATTCCGACTAACGGCAGAATGTAAATAATCAACAGCCAGGCCATCGCGGAGGGAACTGCGCGTCG  >  NC_000913/1308290‑1308555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑