Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,359,363	T→C	intergenic (‑22/+127)	ymjE ← / ← puuA	protein YmjE/glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,359,363	0	T	C	100.0%	175.2 / NA	49	intergenic (‑22/+127)	ymjE/puuA	protein YmjE/glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/21);  total (28/21)

AATCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGT  >  NC_000913/1359236‑1359497                                                                                                                                |                                                                                                                                       aaTCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGAGTTAATCATAGGCTTTACCTCATTTCTTCGTTACAGCCTTAATCGTTaa                                                                                                                            >  2:478506/1‑140 (MQ=255)             ttAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGtt                                                                                                                >  1:92624/1‑140 (MQ=255)               aGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTgc                                                                                                              >  2:685061/1‑140 (MQ=255)                 cAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTgccg                                                                                                            >  1:10040/1‑140 (MQ=255)                   aGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTgccgcc                                                                                                          <  1:526587/140‑1 (MQ=255)                   aGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCCCCACAGCCTTAATCGTTAACGGAAGCCCGTTgccgcc                                                                                                          >  1:871497/1‑140 (MQ=255)                     ggAAGCAAAGGGGGCTGAGTCCTGTCAGTAGCAGGAGTGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGccgcccg                                                                                                        <  1:478506/140‑1 (MQ=255)                       aaGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCcgcg                                                                                                      <  1:625625/140‑1 (MQ=255)                       aaGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCcgcg                                                                                                      <  1:610042/140‑1 (MQ=255)                          cAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTc                                                                                                   <  2:834450/140‑1 (MQ=255)                               ggggCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATaa                                                                                              >  1:39780/1‑140 (MQ=255)                                   cGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCTGTCGCCGCCCGCGCTCCATAAcgcg                                                                                          >  1:441861/1‑140 (MQ=255)                                    ggAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGa                                                                                         >  1:587976/1‑140 (MQ=255)                                      aGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACa                                                                                       <  2:713247/140‑1 (MQ=255)                                         ccAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTTATCGTTAAAGGAGGCCGGTTGCCGCTCGCGGTCCATAACGCGACATcc                                                                                    >  2:328566/1‑140 (MQ=255)                                             tcagtAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGa                                                                                <  2:952540/140‑1 (MQ=255)                                                gtAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCTTTGCCGCACGCGCTACATAACGCGACATCCGGGAAAt                                                                             >  1:324107/1‑140 (MQ=255)                                                 tAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATg                                                                            <  2:39780/140‑1 (MQ=255)                                                 tAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATg                                                                            >  2:121603/1‑140 (MQ=255)                                                  aGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAAAGGAAGCCCGTTGACGCACGCGCTCCATAACCGGACATCCGGGAAATGt                                                                           >  2:488183/1‑140 (MQ=255)                                                       gaggGGCGCATACGCAGACTCCCTCGCCATGAGGGAATTGAATCATCGACATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGa                                                                      <  2:267759/140‑1 (MQ=255)                                                         ggggCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGACCGCGCTACATAACGCGACATCCGGGAAATGTCTGGAtt                                                                    >  2:410165/1‑140 (MQ=255)                                                           ggCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTAc                                                                  >  1:728490/1‑140 (MQ=255)                                                           ggCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTAc                                                                  >  1:516269/1‑140 (MQ=255)                                                             cgcATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGGTCCATAAGGCGACGACCGGGAAATGTCTGGATTACCa                                                                >  1:349717/1‑140 (MQ=255)                                                              gcATACGCAGCCTCCCTCGCCATGCGGGGGTTTAATCCTCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAg                                                               <  1:488183/140‑1 (MQ=255)                                                                      aGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTc                                                       <  2:90981/140‑1 (MQ=255)                                                                      aGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTc                                                       >  1:864324/1‑140 (MQ=255)                                                                        cctccctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAg                                                     >  2:141659/1‑140 (MQ=255)                                                                           ccctcGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGcc                                                  <  2:36837/140‑1 (MQ=255)                                                                           ccctcGCCATGCGGGGACTTAACCATCGGCACCATCTCACTTCCTCGTTACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACGTCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGcc                                                  <  2:349717/140‑1 (MQ=255)                                                                                    tGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTAACAACAAATGACAGGCCTGGCTCCGc                                         >  1:409911/1‑140 (MQ=255)                                                                                      cGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTc                                       >  1:738680/1‑140 (MQ=255)                                                                                        gggATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAg                                     <  2:92624/140‑1 (MQ=255)                                                                                         ggATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAgg                                    <  1:90832/140‑1 (MQ=255)                                                                                                     cGGCATCATCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGATCAGGCCGATGAaacaa                        >  2:346879/1‑140 (MQ=255)                                                                                                        catcatCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAAccc                     >  2:229561/1‑140 (MQ=255)                                                                                                        catcatCTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGAGTACCAGCAAATGGCAGACCTGGCTCGGCTCAGGCCGATGAAACAAccc                     >  2:434678/1‑140 (MQ=255)                                                                                                           catcTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGc                  <  2:738680/140‑1 (MQ=255)                                                                                                            atcTCATTTCCTCGTCAGAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCa                 <  2:441861/140‑1 (MQ=255)                                                                                                            atcTCATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCa                 <  1:121603/140‑1 (MQ=255)                                                                                                                cATTTCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAggg             <  1:781864/140‑1 (MQ=255)                                                                                                                    tCCTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGGTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTAt         >  1:444111/1‑140 (MQ=255)                                                                                                                      cTCGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTa       >  1:596220/1‑140 (MQ=255)                                                                                                                        cGTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcg     >  2:871232/1‑140 (MQ=255)                                                                                                                        cGTCACAGCCTTAATCGTTAACGGAAGCCAGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcg     >  1:567629/1‑140 (MQ=255)                                                                                                                         gTCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcgc    >  1:990945/1‑140 (MQ=255)                                                                                                                          tCACAGCCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTAcgcg   <  2:596220/140‑1 (MQ=255)                                                                                                                           cacaGCCTTAATCGTAAACGGACGCCCGTAGCCGCCCCCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTCCCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGt  <  1:495567/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                       AATCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGT  >  NC_000913/1359236‑1359497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑