Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,667	T→C	intergenic (‑141/+54)	fnr ← / ← ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,667	0	T	C	100.0%	209.7 / NA	58	intergenic (‑141/+54)	fnr/ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/22);  total (36/22)

GCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTC  >  NC_000913/1399533‑1399803                                                                                                                                       |                                                                                                                                         gCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAgg                                                                                                                                     >  1:699284/1‑140 (MQ=255)  cTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAggg                                                                                                                                    >  1:825127/1‑140 (MQ=255)  cTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAggg                                                                                                                                    >  1:129838/1‑140 (MQ=255)  cTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAggg                                                                                                                                    <  1:576044/140‑1 (MQ=255)  cTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATACATGTCTATCTAGTTGAAAACCATTAAGTTGCTAAggg                                                                                                                                    >  1:402840/1‑140 (MQ=255)  cTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATACATGTCTAGCTATTTGAAAACCGTTAAGTTGCTAAggg                                                                                                                                    >  1:308960/1‑140 (MQ=255)     aaGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTaa                                                                                                                                 <  2:35377/140‑1 (MQ=255)     aaGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTaa                                                                                                                                 <  1:868582/140‑1 (MQ=255)       gCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACt                                                                                                                               <  2:129838/140‑1 (MQ=255)       gCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACt                                                                                                                               <  2:778758/140‑1 (MQ=255)       gCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAACTTGCTAAGGGTAACt                                                                                                                               >  1:465163/1‑140 (MQ=255)       gCCGTAATTGATATTCGTCAATCTTCACACCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACt                                                                                                                               <  1:315178/140‑1 (MQ=255)       gCCGTAATTGATATTAGTCAATCTTAACACCTTTTTATGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACt                                                                                                                               <  1:262192/140‑1 (MQ=255)         cGTAATTGATATTTGTCCATTTCAACATCTTCTTAAGGACCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACttt                                                                                                                             <  2:266534/140‑1 (MQ=255)                      tGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGtgtg                                                                                                                >  1:204691/1‑140 (MQ=255)                                  cATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCAAAGGGTAACTTGACGTAAAAGCGTGAACAAGCTGGca                                                                                                    >  1:480365/1‑140 (MQ=255)                                      tttttAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACaaa                                                                                                <  2:465163/140‑1 (MQ=255)                                             ggAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTa                                                                                         <  1:919637/140‑1 (MQ=255)                                             ggAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTa                                                                                         <  1:697668/140‑1 (MQ=255)                                                gcgagtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATg                                                                                      >  2:650689/4‑140 (MQ=255)                                                 cAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGt                                                                                     >  2:691642/1‑140 (MQ=255)                                                    gtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTa                                                                                  <  2:825876/140‑1 (MQ=255)                                                       agtCTAAGCAAACCTTATCAGCTGAGAATTCCGATTTTACATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAg                                                                               <  2:308960/140‑1 (MQ=255)                                                                            cagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgcg                                                          >  1:599848/1‑140 (MQ=255)                                                                              gagaATTCCGATATTAGGTGTAAATATATGTTTATCTATTTGAAAACCGTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCa                                                        <  2:357406/140‑1 (MQ=255)                                                                              gagaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCa                                                               <  2:904761/133‑1 (MQ=255)                                                                              gagaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCa                                                               >  1:904761/1‑133 (MQ=255)                                                                              gagaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGATGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTATTTAATGATTAGAGC‑AAATATAACCTTCATGGCGCaa                                                       >  1:495849/1‑140 (MQ=255)                                                                                gaATTCCGATATTAGAAGTAAAGATATGTCTTTCTTTTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAAt                                                      <  2:480365/140‑1 (MQ=255)                                                                                   ttCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAAc                                                   <  2:825127/140‑1 (MQ=255)                                                                                    tCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgcgc                                                         <  1:874778/133‑1 (MQ=255)                                                                                    tCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgcgc                                                         >  2:874778/1‑133 (MQ=255)                                                                                           ttAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCttt                                           >  2:3753/1‑140 (MQ=255)                                                                                           ttAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCttt                                           >  1:51420/1‑140 (MQ=255)                                                                                             aGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTc                                         >  1:925479/1‑140 (MQ=255)                                                                                                  tAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGa                                    >  2:1999/1‑140 (MQ=255)                                                                                                         atGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGc                             >  2:922106/1‑140 (MQ=255)                                                                                                          tGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGcc                            >  2:740735/1‑140 (MQ=255)                                                                                                          tGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGcc                            <  2:699284/140‑1 (MQ=255)                                                                                                              tatctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATGACCGCTGTTTTCGGTGAACTCCGCCTGCa                        >  1:261823/1‑140 (MQ=255)                                                                                                              tatctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGaa                                   >  2:731435/1‑129 (MQ=255)                                                                                                              tatctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGaa                                   <  1:731435/129‑1 (MQ=255)                                                                                                              tatctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCg                              >  1:947575/1‑134 (MQ=255)                                                                                                              tatctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCg                              <  2:947575/134‑1 (MQ=255)                                                                                                              tatctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCa                        >  1:134483/1‑140 (MQ=255)                                                                                                                tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGTGCAATAATCACACTGTTCGCTGAACTCCGCCTGCata                      >  1:313626/1‑140 (MQ=255)                                                                                                                tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                      >  2:781420/1‑140 (MQ=255)                                                                                                                tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                      >  2:920996/1‑140 (MQ=255)                                                                                                                tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                      >  2:973710/1‑140 (MQ=255)                                                                                                                tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                      >  2:40316/1‑140 (MQ=255)                                                                                                                tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                      <  2:204691/140‑1 (MQ=255)                                                                                                                tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                      >  1:996007/1‑140 (MQ=255)                                                                                                                 ctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAGCCTTCATGGCGCGATAGCCACTCTTGTCGCAGAACTCCGCCTGCatat                     >  2:273273/1‑140 (MQ=255)                                                                                                                      tGAAAAACCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCATTAACCACACTTTTCGCAGAACCCCGCCTGCATATCCGGt                >  2:342001/1‑140 (MQ=255)                                                                                                                        aaaaCCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCa              >  1:572276/1‑140 (MQ=255)                                                                                                                            cccTTAAGTTGCTAAGGGAAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGt          >  1:817544/1‑140 (MQ=255)                                                                                                                              cTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCGTTTCGCTAAACTCCGCCTGCAGATCCGGTCATGGTGc        >  1:427807/1‑140 (MQ=255)                                                                                                                                tAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCg      >  1:170886/1‑140 (MQ=255)                                                                                                                                    ttgctAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTc  <  2:599848/140‑1 (MQ=255)                                                                                                                                    ttgctAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTc  <  1:650689/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTC  >  NC_000913/1399533‑1399803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑