| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,405,021 | C→T | A94V (GCA→GTA) | abgR → | putative LysR‑type DNA‑binding transcriptional regulator AbgR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,405,021 | 0 | C | T | 97.7% | 136.2 / NA | 43 | A94V (GCA→GTA) | abgR | putative LysR‑type DNA‑binding transcriptional regulator AbgR |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (18/24); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGCAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGATTAATG > NC_000913/1404884‑1405155 | ggcgCAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTggtag > 2:975078/1‑140 (MQ=255) cgcAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTggtaggg < 2:245319/140‑1 (MQ=255) gcAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTggtagggc > 1:197977/1‑140 (MQ=255) aCTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGa < 2:73393/140‑1 (MQ=255) aCTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAAATGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGa < 2:581044/140‑1 (MQ=255) tttttCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCTCAAGAGGAAATTTGCCAACGACAAGTGCAACTGGTAGGGCAGATTAat > 2:415760/1‑140 (MQ=255) ttCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATc > 2:1021969/1‑140 (MQ=255) ccGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCa < 2:131342/140‑1 (MQ=255) cGTAGTAATGGCGTGAATTTAATTGATGGCGTTGATAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCAt < 2:477489/140‑1 (MQ=255) cGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCAt < 2:57600/140‑1 (MQ=255) gtagtaAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATg < 1:922130/140‑1 (MQ=255) taAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATggggg < 1:573909/140‑1 (MQ=255) taAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATggggg < 1:513139/140‑1 (MQ=255) aaaGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGc < 1:714017/140‑1 (MQ=255) gTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTAt > 1:85139/1‑140 (MQ=255) tGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGCCAAGGGCAGCTGGTGGGGCAGATTAATATCGACATGGGGGCCAGTAtt > 2:480035/1‑140 (MQ=255) tGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTAtt > 1:685739/1‑140 (MQ=255) aCTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGAGCACAATAATATCGGCGTGGGGACCAGCATTTCCCGCAg > 2:465405/1‑140 (MQ=255) gCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGAt < 1:902157/140‑1 (MQ=255) gCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGAATGGGGGCCAGTATTTCCCGCAGTCTGAt > 1:339010/1‑140 (MQ=255) gCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAACATCGGCATGGGGGCCAGCATTTCCCGCAGTCTGAt > 2:433619/1‑140 (MQ=255) ttATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCatat > 2:200736/1‑140 (MQ=255) tCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATcagc > 2:878314/1‑140 (MQ=255) ttCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCAt > 1:70138/1‑140 (MQ=255) cTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATcc < 2:85139/140‑1 (MQ=255) cTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATcc < 1:1021969/140‑1 (MQ=255) cTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATcc < 2:232288/140‑1 (MQ=255) tGAAGAGCTGCGCGAAAACCAATAGGATATTTGGCGACGTCAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGc < 2:464281/140‑1 (MQ=255) agCTGCGCGCAGCCCGAGAGGAAATACGCTAACGACAGGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTa < 1:433619/140‑1 (MQ=255) cgcgcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGt < 1:975078/140‑1 (MQ=255) gcgcAGCCCAAGAGGCTATTCGTCATCGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTa < 1:480035/140‑1 (MQ=255) gcAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACg < 1:878314/140‑1 (MQ=255) aGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCa > 2:111820/1‑140 (MQ=255) agagGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATgg < 2:543174/140‑1 (MQ=255) gagGATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGa < 1:200736/140‑1 (MQ=255) ggATATTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCATGTAAAATTACGCATTATGGAAg > 1:353929/1‑140 (MQ=255) tatTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGc > 1:956276/1‑140 (MQ=255) tatTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGc > 1:695589/1‑140 (MQ=255) tatTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGc > 2:723447/1‑140 (MQ=255) tatTCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCAT‑GGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGGAGCATCCTCAGCTAGAAGTACTCATTATGGAAGGGCa > 1:320950/1‑140 (MQ=255) ttCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATAACGGCATGGTGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCaa < 2:339010/140‑1 (MQ=255) tCGCCAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAAc < 2:888998/140‑1 (MQ=255) ccAACGACAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCACCCGCAGGGAAAAGTGCGCATTATGGAAGGGCAACTgg > 1:297178/1‑140 (MQ=255) aCAAGGGCACCTGGTAGGGCAATTTAATATGTGCAT‑GGGTCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgat < 2:320950/140‑1 (MQ=255) cAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgat < 2:685739/140‑1 (MQ=255) cAAGGGCAACTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCgat < 2:695589/140‑1 (MQ=255) aCTGGTAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGCCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGATTAATg > 1:820921/1‑140 (MQ=255) | GGCGCAACTCTTTTTTCGCCGTAGTAAAGGCGTGACGTTAACTGATGCCGGTGAAAGTTTTTATCAGCACGCCAGTCTAATTCTTGAAGAGCTGCGCGCAGCCCAAGAGGATATTCGCCAACGACAAGGGCAACTGGCAGGGCAGATTAATATCGGCATGGGGGCCAGTATTTCCCGCAGTCTGATGCCAGCTGTCATATCTCGTTTTCATCAGCAGCATCCGCAGGTAAAAGTACGCATTATGGAAGGGCAACTGGTGTCGATGATTAATG > NC_000913/1404884‑1405155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |