Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,442,261	A→G	F195L (TTC→CTC)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,442,261	0	A	G	100.0%	150.8 / NA	42	F195L (TTC→CTC)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

AATCAATGCACCGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATA  >  NC_000913/1442130‑1442397                                                                                                                                    |                                                                                                                                         aatcaatGCACCGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTg                                                                                                                                  >  2:536806/1‑140 (MQ=255)     aatGCACCGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAg                                                                                                                              <  2:1020044/140‑1 (MQ=255)       tGCACCGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGAt                                                                                                                            >  2:197879/1‑140 (MQ=255)         cACCGCGACTGGTATAGACGATTATTACGACATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCg                                                                                                                          <  1:296552/140‑1 (MQ=255)           ccGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGAc                                                                                                                        <  2:720268/140‑1 (MQ=255)           ccGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGAc                                                                                                                        <  2:567825/140‑1 (MQ=255)           ccGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGAc                                                                                                                        <  1:590371/140‑1 (MQ=255)            cgcgACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACa                                                                                                                       >  2:210077/1‑140 (MQ=255)            cgcgACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACa                                                                                                                       <  1:637415/140‑1 (MQ=255)             gcgACTGGTATTGACGATCACCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACAt                                                                                                                      >  1:73496/1‑140 (MQ=255)                    gTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTc                                                                                                                  >  1:17009/1‑137 (MQ=255)                    gTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTc                                                                                                                  <  2:17009/137‑1 (MQ=255)                              tcatcaCGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTcc                                                                                                     >  1:123263/1‑140 (MQ=255)                               catcaCGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCa                                                                                                    >  1:653218/1‑140 (MQ=255)                               catcaCGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCa                                                                                                    >  1:852230/1‑140 (MQ=255)                                atcaCGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAg                                                                                                   <  2:224480/140‑1 (MQ=255)                                            ttCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACt                                                                                       <  2:195727/140‑1 (MQ=255)                                            gagacaggTTCGAAGACGGCTTCGTACAACAGATGATCGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACt                                                                                       <  1:260265/133‑1 (MQ=255)                                                           gcggcTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGaa                                                                        <  2:73496/140‑1 (MQ=255)                                                            cggcTTCGTTCAACATATGATAGTTATCCAGTGTCAGGGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAAc                                                                       <  1:478124/140‑1 (MQ=255)                                                              gcTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATGCTCCACACGGAGGTCCGGCGCCGCTGCACTTGGATACGGATctgtctc                                                                     >  2:260265/1‑134 (MQ=255)                                                                       aaCAGATGATAGTTTTCCGGTGTCAGCGGGCTGTGTAGACAGATAACGCCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACg                                                            <  2:302100/140‑1 (MQ=255)                                                                            atgatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATAc                                                       >  1:652823/1‑140 (MQ=255)                                                                            atgatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATAc                                                       >  2:697781/1‑140 (MQ=255)                                                                              gatAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCa                                                     <  2:659526/140‑1 (MQ=255)                                                                                tAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCaaa                                                   <  2:123263/140‑1 (MQ=255)                                                                                 aGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGGGCCGCTGCTCTTTGATACGGATCGACCGCCGGCAGACGCATACCaaaa                                                  >  2:307355/1‑140 (MQ=255)                                                                                 aGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCaaaa                                                  >  2:796338/1‑140 (MQ=255)                                                                                          gtgtCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGa                                         >  1:568264/1‑140 (MQ=255)                                                                                             tCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATg                                      >  1:736676/1‑140 (MQ=255)                                                                                             tCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATg                                      <  1:210077/140‑1 (MQ=255)                                                                                               aGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATgcg                                    >  1:120662/1‑140 (MQ=255)                                                                                                       gTGCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCg                            >  1:895473/1‑140 (MQ=255)                                                                                                         gCAGAGAGATAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAAACGCATACCAATACGTTTCAGAATGCGCAGCATCGcc                          >  1:343050/1‑140 (MQ=255)                                                                                                                  tAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGAtt                 >  1:655296/1‑140 (MQ=255)                                                                                                                  tAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGAtt                 >  2:55290/1‑140 (MQ=255)                                                                                                                  tAACGTCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCAAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGAtt                 >  2:620691/1‑140 (MQ=255)                                                                                                                      gTCTGATTCAGAGAGCAAGGTTCGCAGATCGCCATCCTCTACACCGAGCTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTAc             <  2:343050/140‑1 (MQ=255)                                                                                                                       tCTGATTCAGAGAGCAGGGTTGGCAGATCTACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTAcc            >  1:789148/1‑140 (MQ=255)                                                                                                                       tCTGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTAcc            >  1:162748/1‑140 (MQ=255)                                                                                                                         tGATTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCgg          >  1:45897/1‑140 (MQ=255)                                                                                                                           aTTCAGAGAGCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTa        <  1:545486/140‑1 (MQ=255)                                                                                                                                 agagCAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATa  >  2:758573/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         AATCAATGCACCGCGACTGGTATTGACGATCATCACGCCATTTTTCATCTGTTCGAAGGCGGCTTCGTTCAACAGATGATAGTTTTCCGGTGTCAGCGGGCAGTGCAGAGAGATAACGTCTGATTCAGAGAACAGGGTTGGCAGATCGACATACTCCACACCGAGTTCCAGCGCCGCTGCACTTGGATACGGATCGAACGCCAGCAGACGCATACCAAAACCTTTCAGAATGCGCAGCATCGCCACACCGATTTTACCGGTACCGATA  >  NC_000913/1442130‑1442397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑