Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,521,361	T→C	T569A (ACC→GCC)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,521,361	0	T	C	100.0%	127.3 / NA	36	T569A (ACC→GCC)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

TGCCTGCATACCAACCATCTTCCGGTACATAACCTATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGTCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCAT  >  NC_000913/1521226‑1521495                                                                                                                                        |                                                                                                                                       tGCCTGCATACCAACCATCTTCCGGTACATAACCTATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGA‑‑‑‑‑‑TTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGAcgcg                                                                                                                              >  1:714888/1‑140 (MQ=255)                      ccGGTACATAACCTATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTAGCATCAAGCCAGGCCCCGGACGCGTTTACGCGAAAAtct                                                                                                               >  2:398093/1‑140 (MQ=255)                          tACATAACCTATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTg                                                                                                           >  1:955974/1‑140 (MQ=255)                             aTAACCTATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGa                                                                                                        <  1:165581/140‑1 (MQ=255)                                   tATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGtt                                                                                                  <  1:852704/140‑1 (MQ=255)                                   tATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGtt                                                                                                  <  1:829385/140‑1 (MQ=255)                                    aTCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTg                                                                                                 >  1:718959/1‑140 (MQ=255)                                        aCGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATcc                                                                                             <  1:697921/140‑1 (MQ=255)                                        aCGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATcc                                                                                             <  2:566118/140‑1 (MQ=255)                                                      ttACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAgc                                                                               <  2:955974/140‑1 (MQ=255)                                                          gggCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCt                                                                           >  1:922555/1‑140 (MQ=255)                                                               aTCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTgacg                                                                      >  1:532897/1‑140 (MQ=255)                                                               aTCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCACTTCAGCGCCTTgacg                                                                      >  1:452483/1‑140 (MQ=255)                                                                tCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGTACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTgacga                                                                     >  1:13373/1‑140 (MQ=255)                                                                tCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTgacga                                                                     >  1:170987/1‑140 (MQ=255)                                                                      gCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGGGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTc                                                               >  1:887617/1‑140 (MQ=255)                                                                          ccGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTTCGAAACGTCGATGCCATGCCAGTTCCGCGCTTTGACGACGGGTCTTTc                                                           >  2:368751/1‑140 (MQ=255)                                                                               acaggTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCa                                                      <  2:189482/136‑1 (MQ=255)                                                                                    ttACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCAttttt                                                 >  1:975779/1‑140 (MQ=255)                                                                                            cTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATTCCAGTTCAGCGCATTGACGACGGGTGTTTCGGGCATTTTTGTAAGTCg                                         >  2:286591/1‑140 (MQ=255)                                                                                            cTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCg                                         >  2:183615/1‑140 (MQ=255)                                                                                               ttCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTAc                                      >  2:707952/1‑140 (MQ=255)                                                                                                tCATTGCATACATTGCGGCGATTGGTCGCATAAAGCCAGACCCAGGACTCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACg                                     <  2:452483/140‑1 (MQ=255)                                                                                                tCATTGCAAACATTGCTCCGATAGGTCGCATGAAGTCAGCCCCAGGACTCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACg                                     <  2:333953/140‑1 (MQ=255)                                                                                                   ttGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGccc                                  <  2:975779/140‑1 (MQ=255)                                                                                                    tGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACAACGGGTCATTCCGGCATTTTTGTAAGTCGTACGCCCa                                 >  1:333953/1‑140 (MQ=255)                                                                                                         aCATTGCTGCGATAGGTCGTATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCt                            >  2:751046/1‑140 (MQ=255)                                                                                                              gctgcGATAGGTCGCATCAAGTCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGc                       >  2:256375/1‑140 (MQ=255)                                                                                                               ctgcGATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCt                      <  2:714888/140‑1 (MQ=255)                                                                                                                   gATAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCg                  >  1:48723/1‑140 (MQ=255)                                                                                                                     tAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATc                   >  1:7802/1‑137 (MQ=255)                                                                                                                     tAGGTCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATc                   <  2:7802/137‑1 (MQ=255)                                                                                                                         tCGCATCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGacaaca            <  1:707952/140‑1 (MQ=255)                                                                                                                              tCAAGCCAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTtc       <  1:932689/140‑1 (MQ=255)                                                                                                                                   ccAGGCCCAGGACGCTTTTACGCGACAATATCCTGCGCAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTtcatcat  <  1:368751/140‑1 (MQ=255)                                                                                                                                   ccAGGCCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTtcatcat  <  1:33536/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TGCCTGCATACCAACCATCTTCCGGTACATAACCTATCGACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATTGCAAACATTGCTGCGATAGGTCGCATCAAGCCAGGTCCAGGACGCGTTTACGCGAAAATCTCCTGCGAAACGTTGATCCCATGCCAGTTCAGCGCCTTGACGACGGGTCTTTCCGGCATTTTTGTAAGTCGTACGCCCACCGCTACTGCTATCGACAACAATTTCATCAT  >  NC_000913/1521226‑1521495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑