Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	144,822	A→G	K82K (AAA→AAG)	yadI →	putative PTS enzyme IIA component YadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,822	0	A	G	100.0%	194.0 / NA	56	K82K (AAA→AAG)	yadI	putative PTS enzyme IIA component YadI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/28);  total (28/28)

AGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTT  >  NC_000913/144694‑144957                                                                                                                                 |                                                                                                                                        aGCTCTAATATGCTCAGTCGCATG‑TGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGc                                                                                                                             <  1:38932/140‑1 (MQ=255)  cagctAATATGCTCAGCCGCATCATGTGCGAAGCTCTGCATGAAGCGGCCTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGc                                                                                                                             <  2:380418/137‑1 (MQ=255)     ctAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGaa                                                                                                                          <  1:807002/140‑1 (MQ=255)       aaTATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGt                                                                                                                        >  1:93588/1‑140 (MQ=255)       aaTATGCTCAGCCGCATGATGTACGATGCGCTGCATGTAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGt                                                                                                                        <  1:463366/140‑1 (MQ=255)            gCTCAGCCGCATGATGTGCGATTCTCTGCATAAAGTGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGAttt                                                                                                                   <  2:341958/140‑1 (MQ=255)             cTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTc                                                                                                                  >  1:841323/1‑140 (MQ=255)              tCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATAATTAAGCCACACGCGCTCCCGTTGCGAAGTGATTTCt                                                                                                                 >  1:341958/1‑140 (MQ=255)                aGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTgg                                                                                                               >  1:644869/1‑140 (MQ=255)                aGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTgg                                                                                                               >  1:789619/1‑140 (MQ=255)                aGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTACTAAGCCAGAAGGACACCCGTTGCGAAGTGATTTcctg                                                                                                               >  1:380418/1‑137 (MQ=255)                  ccGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGgtg                                                                                                             >  1:781575/1‑140 (MQ=255)                  ccGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCCGgtg                                                                                                             >  1:513880/1‑140 (MQ=255)                   cGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGgtgt                                                                                                            <  2:938295/140‑1 (MQ=255)                       tgatgTGCGATGCTCTGGATGACGCGGACTCTGGTAAGGGTGTCATCTCCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACg                                                                                                        <  2:291637/140‑1 (MQ=255)                       tgatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACg                                                                                                        >  2:864193/1‑140 (MQ=255)                            tgCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATCAAGCCACACGCATTCCCGTTGCGAATTGATTTCTGGTGTCACGTTAcc                                                                                                   >  2:347702/1‑140 (MQ=255)                              cGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGt                                                                                                 >  1:871907/1‑140 (MQ=255)                              cGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGt                                                                                                 >  1:840978/1‑140 (MQ=255)                                   ctctGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAAtt                                                                                            >  1:955891/1‑140 (MQ=255)                                      tGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGaa                                                                                         <  2:841323/140‑1 (MQ=255)                                       gCATGAAGCGGACTCTGCTGAGAGTGTCATCCTCTAAACTGATATAGCTGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAAc                                                                                        <  2:265328/140‑1 (MQ=255)                                             aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAgatga                                                                                  >  2:769115/1‑140 (MQ=255)                                             aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAgatga                                                                                  >  1:560906/1‑140 (MQ=255)                                             aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAgatga                                                                                  <  2:644869/140‑1 (MQ=255)                                                 gACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGc                                                                              <  2:955891/140‑1 (MQ=255)                                                  aCTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCt                                                                             >  2:548538/1‑140 (MQ=255)                                                         tGAGGGTGTCATCTTCTTAACCTATATAGCCCGAGCGCCCCCGTATCGTGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTg                                                                      <  2:292528/140‑1 (MQ=255)                                                         tGAGGGTGTCATCTTCTTAACCGATATAGTCGGAGCGTCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTg                                                                      <  1:403650/140‑1 (MQ=255)                                                         tGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTg                                                                      <  1:136490/140‑1 (MQ=255)                                                         tGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTg                                                                      <  1:864193/140‑1 (MQ=255)                                                              gtgtCATCTTATTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAAcc                                                                 >  2:137529/1‑140 (MQ=255)                                                                gtCATCTTCTTAACCGATATAGTCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAACGTTAATGGAATAGCTGATGGATTGCCGTGAAACCAt                                                               >  1:475679/1‑140 (MQ=255)                                                                gtCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCAt                                                               >  1:71572/1‑140 (MQ=255)                                                                  cATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGa                                                             >  1:637925/1‑140 (MQ=255)                                                                   aTCTTCTTAACCGATCTAGCCGCAGCGCCTCCGTATCCCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAc                                                            <  1:279139/140‑1 (MQ=255)                                                                   aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAc                                                            <  2:789619/140‑1 (MQ=255)                                                                   aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAc                                                            <  2:701646/140‑1 (MQ=255)                                                                           aaCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCag                                                    >  1:690830/1‑140 (MQ=255)                                                                                atatAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGGTTGCCGTGGAACCGTGACCAGTTCAGAGttt                                               >  2:264916/1‑140 (MQ=255)                                                                                  ataGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTcg                                             <  2:71572/140‑1 (MQ=255)                                                                                   taGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTcgc                                            <  2:871907/140‑1 (MQ=255)                                                                                   taGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTcgc                                            <  2:840978/140‑1 (MQ=255)                                                                                             gcCACCGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTg                                  <  2:1005653/140‑1 (MQ=255)                                                                                                  cGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGaa                             <  2:130596/140‑1 (MQ=255)                                                                                                  cGTATCGCGTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGaa                             <  1:137529/140‑1 (MQ=255)                                                                                                         cgTGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGCGTTACGCGAGCGTGTTGTCGAACTGGGTg                      >  1:471252/1‑140 (MQ=255)                                                                                                           tGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcg                    >  2:550361/1‑140 (MQ=255)                                                                                                           tGGCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcg                    >  1:815815/1‑140 (MQ=255)                                                                                                            ggCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcgc                   >  2:131769/1‑140 (MQ=255)                                                                                                            ggCTTCATTATTAAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcgc                   <  2:93588/140‑1 (MQ=255)                                                                                                            ggCTTCATTATTAAGCCACAAGCACCCCCGGTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcgc                   <  1:702282/140‑1 (MQ=255)                                                                                                                      ttaAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGag         <  2:108345/140‑1 (MQ=255)                                                                                                                       taAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcgc                   >  2:810956/1‑129 (MQ=255)                                                                                                                       taAGCCACAAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcgc                   <  1:810956/129‑1 (MQ=255)                                                                                                                             acaAGCACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCtt  >  2:67468/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                        AGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTT  >  NC_000913/144694‑144957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑