Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,577,857	C→T	A496T (GCC→ACC)	yddA ←	ABC transporter family protein YddA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,577,857	0	C	T	100.0%	170.3 / NA	53	A496T (GCC→ACC)	yddA	ABC transporter family protein YddA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

AACCATAATGACGCCGCTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTG  >  NC_000913/1577720‑1577991                                                                                                                                          |                                                                                                                                       aaCCATAATGACGCCGCTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGa                                                                                                                                      >  2:903012/1‑140 (MQ=255)        aTGACGCCGCTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACgttg                                                                                                                               >  1:48758/1‑140 (MQ=255)             gccgcTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGttttt                                                                                                                          >  2:563461/1‑140 (MQ=255)               cgcTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTGATAAAATTAATCGTGGCAGGGTGATACGTTGTTTTTcg                                                                                                                        >  1:288450/1‑140 (MQ=255)               cgcTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTcg                                                                                                                        >  1:717623/1‑140 (MQ=255)                 cTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTcgcc                                                                                                                      >  1:948656/1‑140 (MQ=255)                  ttGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTcgccg                                                                                                                     <  2:572815/140‑1 (MQ=255)                      gggTAGTTTTTCACGCACTAAACGCAGTAAGGGGAAAGCATCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTgg                                                                                                                 <  2:418551/140‑1 (MQ=255)                       ggTAGTTTTTCACGCCCTAAACGCACTAAGCGGATAGACTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGa                                                                                                                <  1:275484/140‑1 (MQ=255)                         tAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGaaa                                                                                                              >  2:739067/1‑140 (MQ=255)                               ttCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAatat                                                                                                        <  1:903012/140‑1 (MQ=255)                                tCACGCACTAAACGCTGTAAGCGGACAGCATCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATAtc                                                                                                       <  1:425915/140‑1 (MQ=255)                                  aCGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATAtctc                                                                                                     >  2:784772/1‑140 (MQ=255)                                   cGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTcc                                                                                                    >  1:39217/1‑140 (MQ=255)                                        tAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGCAAAATTAATCGTCCCAGGGTGGTACGTTGGTTTTCGCCGCTGGGAAGAATATCTCCCCAGc                                                                                               >  2:273258/1‑140 (MQ=255)                                           aCGCAGTAAGCGGATAGCCTCTCGTTCCTCATGATAAGATGTAGTTTCCTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGAt                                                                                            <  2:288450/140‑1 (MQ=255)                                              cAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGt                                                                                         <  1:125162/140‑1 (MQ=255)                                                 tAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGt                                                                                      <  1:563461/140‑1 (MQ=255)                                                  aaGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTc                                                                                     >  2:501728/1‑140 (MQ=255)                                                  aaGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTc                                                                                     >  2:11027/1‑140 (MQ=255)                                                  aaGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGACACGTTGTTATTCACCGCTGGAAAGAGTATCTCCCCAGCGGTCGTGGTc                                                                                     >  2:317134/1‑140 (MQ=255)                                                               ctTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCa                                                                        >  2:662357/1‑140 (MQ=255)                                                               ctTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCa                                                                        <  1:963434/140‑1 (MQ=255)                                                               ctTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCa                                                                        <  1:912188/140‑1 (MQ=255)                                                                     ccTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAAt                                                                  >  2:37097/1‑140 (MQ=255)                                                                       tCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAAttt                                                                >  1:745055/1‑140 (MQ=255)                                                                         aaGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTcc                                                              <  2:39217/140‑1 (MQ=255)                                                                               agagGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGAc                                                        <  1:501728/140‑1 (MQ=255)                                                                               agagGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGAc                                                        <  1:872536/140‑1 (MQ=255)                                                                                gagGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGAcc                                                       >  2:147624/1‑140 (MQ=255)                                                                                gagGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGAc                                                        <  2:903875/139‑1 (MQ=255)                                                                                gagGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGAc                                                        >  1:903875/1‑139 (MQ=255)                                                                                   gTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAAc                                                    >  2:562801/1‑140 (MQ=255)                                                                                    tAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAAcc                                                   <  2:717623/140‑1 (MQ=255)                                                                                    tAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAAcc                                                   <  1:739067/140‑1 (MQ=255)                                                                                     aGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCt                                                  >  2:220164/1‑140 (MQ=255)                                                                                      gTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTg                                                 <  2:566418/140‑1 (MQ=255)                                                                                      gTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTg                                                 <  1:147624/140‑1 (MQ=255)                                                                                      gTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTg                                                 <  2:108547/140‑1 (MQ=255)                                                                                                       ccATTTCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCa                                <  2:948656/140‑1 (MQ=255)                                                                                                           ttCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGa                            >  1:925156/1‑140 (MQ=255)                                                                                                           ttCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGGCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGa                            >  1:107088/1‑140 (MQ=255)                                                                                                            tCGGACGTCGTAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGAt                           >  2:939711/1‑140 (MQ=255)                                                                                                                    cgtAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCt                                  >  2:186057/1‑125 (MQ=255)                                                                                                                    cgtAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCt                                  <  1:186057/125‑1 (MQ=255)                                                                                                                    cgtAAAATTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTc                   >  2:1016401/1‑140 (MQ=255)                                                                                                                          aTTAATCGTGCCAGGTTGATACTTTCTTTCTCGCCGCTGGAAAGAATATCTCCCCAGCGGTCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACggg             <  1:317134/140‑1 (MQ=255)                                                                                                                          aTTAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACggg             <  1:11027/140‑1 (MQ=255)                                                                                                                           ttAATCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGc            <  2:48758/140‑1 (MQ=255)                                                                                                                              aTCGTGCCAGGGTGCTACGTTGTTTTGCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAgg         >  2:1019750/1‑140 (MQ=255)                                                                                                                              aTCGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAgg         >  1:911954/1‑140 (MQ=255)                                                                                                                                cGTGCCAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAgggg       <  2:925156/140‑1 (MQ=255)                                                                                                                                    ccAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGt   <  2:107088/140‑1 (MQ=255)                                                                                                                                     cAGGGTGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTg  >  1:641407/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AACCATAATGACGCCGCTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAGAAATATCCATTTCGGACGTCGTAAAATTAATCGTGCCAGGGCGATACGTTGTTTTTCGCCGCTGGAAAGAATATCTCCCCAGCGATCGTGGTCATGAATACGCGCAGCCAATTTCCCAAGACCAACCTGATGCAGTACTTCGCTCAACGATTTATCGTCTACGGGCAGGGGAAGTG  >  NC_000913/1577720‑1577991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑